Streptococcus phage phi-SsuJS7_SSU0237

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3MNA5|A0A7G3MNA5_9CAUD Uncharacterized protein OS=Streptococcus phage phi-SsuJS7_SSU0237 OX=2664400 PE=4 SV=1
MM1 pKa = 7.52SLQIEE6 pKa = 4.67KK7 pKa = 10.34FGHH10 pKa = 6.13AKK12 pKa = 10.02IEE14 pKa = 4.96DD15 pKa = 3.22IHH17 pKa = 7.54EE18 pKa = 4.27LEE20 pKa = 4.22KK21 pKa = 11.01EE22 pKa = 4.22FNIVLPKK29 pKa = 10.35DD30 pKa = 3.74YY31 pKa = 10.97KK32 pKa = 11.07QFLLDD37 pKa = 3.71FNGGEE42 pKa = 4.28TIDD45 pKa = 3.88YY46 pKa = 8.61EE47 pKa = 4.38LNKK50 pKa = 10.15ISIPHH55 pKa = 6.37SDD57 pKa = 3.53GEE59 pKa = 4.43INLDD63 pKa = 3.5LLFGVRR69 pKa = 11.84PDD71 pKa = 3.66EE72 pKa = 4.12QGTDD76 pKa = 2.68IGKK79 pKa = 7.83WTEE82 pKa = 3.87EE83 pKa = 4.23YY84 pKa = 10.2IDD86 pKa = 5.87DD87 pKa = 4.07ILEE90 pKa = 4.13HH91 pKa = 7.24SLIIGDD97 pKa = 4.07TVQHH101 pKa = 5.92GFIIIWISGDD111 pKa = 3.5EE112 pKa = 4.21NEE114 pKa = 4.49GVYY117 pKa = 10.81YY118 pKa = 10.89YY119 pKa = 11.13DD120 pKa = 3.65DD121 pKa = 4.29TYY123 pKa = 11.66TLASSSDD130 pKa = 3.39GGNTYY135 pKa = 11.03YY136 pKa = 11.05LAPTFTEE143 pKa = 5.21FLALLTPP150 pKa = 4.92

Molecular weight: 17.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3ML69|A0A7G3ML69_9CAUD DUF4314 domain-containing protein OS=Streptococcus phage phi-SsuJS7_SSU0237 OX=2664400 PE=4 SV=1
MM1 pKa = 7.72MYY3 pKa = 10.16TILTCTIMGLWVLIGLYY20 pKa = 9.77FGYY23 pKa = 8.1MTIRR27 pKa = 11.84DD28 pKa = 4.32DD29 pKa = 3.97IRR31 pKa = 11.84NEE33 pKa = 3.83MEE35 pKa = 4.95RR36 pKa = 11.84KK37 pKa = 9.5AKK39 pKa = 9.36QNKK42 pKa = 8.32EE43 pKa = 4.09KK44 pKa = 10.83LSQTPLSRR52 pKa = 11.84KK53 pKa = 9.46NKK55 pKa = 9.68

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

14405

43

1503

277.0

30.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.671 ± 0.473

0.916 ± 0.145

5.859 ± 0.262

7.331 ± 0.326

3.776 ± 0.184

6.505 ± 0.293

1.951 ± 0.125

6.359 ± 0.33

6.852 ± 0.198

8.983 ± 0.348

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.19

4.311 ± 0.177

3.041 ± 0.188

4.436 ± 0.221

4.575 ± 0.3

6.526 ± 0.455

6.38 ± 0.451

6.359 ± 0.257

1.388 ± 0.096

3.742 ± 0.326
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski