Vibrio phage KSF1

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Capistrivirus; Vibrio virus KSF1

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q64EV6|Q64EV6_9VIRU Uncharacterized protein ORF III OS=Vibrio phage KSF1 OX=292443 GN=ORF III PE=4 SV=1
MM1 pKa = 7.47NPITEE6 pKa = 4.22NFKK9 pKa = 10.94GAIASGQANYY19 pKa = 10.21TMVVNGVIGLAAIALGLGWYY39 pKa = 8.31PWYY42 pKa = 10.93LEE44 pKa = 4.35VIVMVAFVSDD54 pKa = 3.46ALTVVVAVAYY64 pKa = 9.31FMAFAYY70 pKa = 9.99GFYY73 pKa = 10.09TGVNASS79 pKa = 3.29

Molecular weight: 8.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q64EU9|Q64EU9_9VIRU Uncharacterized protein ORF X OS=Vibrio phage KSF1 OX=292443 GN=ORF X PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84STFNTTMCAFLMIFTKK20 pKa = 10.54SQEE23 pKa = 3.57LRR25 pKa = 11.84LNVNLKK31 pKa = 9.93KK32 pKa = 10.2IEE34 pKa = 3.94MSFIRR39 pKa = 11.84QPTHH43 pKa = 5.5

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

1723

35

455

143.6

16.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.719 ± 0.852

1.567 ± 0.295

5.223 ± 0.619

3.889 ± 0.511

6.21 ± 0.788

6.558 ± 0.589

2.031 ± 0.549

6.674 ± 0.474

5.746 ± 0.723

9.692 ± 0.611

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.076 ± 0.483

5.514 ± 1.048

4.469 ± 0.616

3.54 ± 0.366

4.469 ± 0.561

6.558 ± 1.145

6.442 ± 0.525

5.34 ± 0.818

1.277 ± 0.26

4.005 ± 0.627
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski