Lactobacillus iners DSM 13335
Average proteome isoelectric point is 7.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1214 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C8PAU3|C8PAU3_9LACO ATP-dependent RecD-like DNA helicase OS=Lactobacillus iners DSM 13335 OX=525328 GN=recD PE=3 SV=1
MM1 pKa = 7.89 DD2 pKa = 4.8 NEE4 pKa = 4.3 LNEE7 pKa = 4.18 MKK9 pKa = 10.23 AQSVAWLKK17 pKa = 11.28 NNLNFYY23 pKa = 10.96 RR24 pKa = 11.84 MDD26 pKa = 3.43 SQTIEE31 pKa = 4.15 VEE33 pKa = 4.25 TPLIDD38 pKa = 4.56 SFGQNIYY45 pKa = 10.94 CFIIKK50 pKa = 9.9 KK51 pKa = 10.32 DD52 pKa = 3.61 EE53 pKa = 4.26 YY54 pKa = 11.52 YY55 pKa = 10.79 LISDD59 pKa = 4.24 GGWFLFKK66 pKa = 10.57 LDD68 pKa = 4.69 PDD70 pKa = 3.73 MSDD73 pKa = 2.93 SDD75 pKa = 4.56 FYY77 pKa = 11.44 NQAVDD82 pKa = 4.13 IIIGTGFEE90 pKa = 3.98 IDD92 pKa = 3.65 EE93 pKa = 4.3 EE94 pKa = 4.31 TGEE97 pKa = 4.2 FFMEE101 pKa = 4.01 VSIDD105 pKa = 3.97 EE106 pKa = 4.92 LPLALNQLAQLQVALSYY123 pKa = 11.23 LKK125 pKa = 10.85
Molecular weight: 14.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.978
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.541
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.872
Patrickios 2.842
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|C8PB19|C8PB19_9LACO Ribonuclease P protein component OS=Lactobacillus iners DSM 13335 OX=525328 GN=rnpA PE=3 SV=1
MM1 pKa = 6.05 TTKK4 pKa = 9.88 RR5 pKa = 11.84 TYY7 pKa = 9.64 QPKK10 pKa = 8.89 KK11 pKa = 7.38 RR12 pKa = 11.84 HH13 pKa = 5.81 RR14 pKa = 11.84 SRR16 pKa = 11.84 VHH18 pKa = 6.84 GFMKK22 pKa = 10.51 RR23 pKa = 11.84 MATANGRR30 pKa = 11.84 KK31 pKa = 9.07 VLARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.2 KK39 pKa = 10.05 GRR41 pKa = 11.84 KK42 pKa = 8.66 VLSAA46 pKa = 4.05
Molecular weight: 5.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1214
0
1214
381740
38
3793
314.4
35.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.748 ± 0.086
0.8 ± 0.024
5.925 ± 0.077
5.601 ± 0.073
4.365 ± 0.061
6.049 ± 0.068
2.015 ± 0.03
8.645 ± 0.097
8.282 ± 0.091
9.676 ± 0.109
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.558 ± 0.039
5.841 ± 0.064
3.283 ± 0.059
3.925 ± 0.041
3.597 ± 0.056
6.036 ± 0.056
5.374 ± 0.089
6.553 ± 0.074
0.828 ± 0.021
3.9 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here