Hypocrea virens (strain Gv29-8 / FGSC 10586) (Gliocladium virens) (Trichoderma virens)
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12389 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9N408|G9N408_HYPVG GST N-terminal domain-containing protein OS=Hypocrea virens (strain Gv29-8 / FGSC 10586) OX=413071 GN=TRIVIDRAFT_68359 PE=4 SV=1
MM1 pKa = 7.64 VLDD4 pKa = 5.72 DD5 pKa = 4.44 EE6 pKa = 5.78 ADD8 pKa = 3.92 PNSSQSPLFGDD19 pKa = 4.62 GFIHH23 pKa = 7.34 PISKK27 pKa = 10.41 SPISRR32 pKa = 11.84 PPVSSIKK39 pKa = 10.57 VFARR43 pKa = 11.84 DD44 pKa = 3.24 LSDD47 pKa = 3.3 YY48 pKa = 11.79 AEE50 pKa = 4.23 FTRR53 pKa = 11.84 EE54 pKa = 4.07 FFCEE58 pKa = 4.09 CDD60 pKa = 3.69 DD61 pKa = 4.65 EE62 pKa = 4.89 KK63 pKa = 10.84 WPPDD67 pKa = 3.96 TPPDD71 pKa = 3.31 ILSNISYY78 pKa = 9.2 PCSCPGGYY86 pKa = 9.2 NAPPPLEE93 pKa = 5.2 IIGSGPNGLVTIGDD107 pKa = 3.9 YY108 pKa = 11.39 SDD110 pKa = 3.31 ATT112 pKa = 3.7
Molecular weight: 12.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.884
IPC_protein 3.846
Toseland 3.63
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.795
Rodwell 3.668
Grimsley 3.541
Solomon 3.834
Lehninger 3.783
Nozaki 3.973
DTASelect 4.215
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.973
Patrickios 0.896
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.849
Protein with the highest isoelectric point:
>tr|G9ME33|G9ME33_HYPVG Uncharacterized protein (Fragment) OS=Hypocrea virens (strain Gv29-8 / FGSC 10586) OX=413071 GN=TRIVIDRAFT_18141 PE=4 SV=1
MM1 pKa = 7.97 PFTTHH6 pKa = 6.61 RR7 pKa = 11.84 RR8 pKa = 11.84 HH9 pKa = 5.76 HH10 pKa = 6.31 HH11 pKa = 4.84 TTTTTTARR19 pKa = 11.84 PYY21 pKa = 10.08 RR22 pKa = 11.84 RR23 pKa = 11.84 SIFSRR28 pKa = 11.84 RR29 pKa = 11.84 APRR32 pKa = 11.84 VHH34 pKa = 6.38 HH35 pKa = 5.5 QRR37 pKa = 11.84 KK38 pKa = 6.91 PTLKK42 pKa = 10.45 DD43 pKa = 3.27 KK44 pKa = 11.46 VSGALTRR51 pKa = 11.84 LRR53 pKa = 11.84 GSLTRR58 pKa = 11.84 RR59 pKa = 11.84 PGVKK63 pKa = 9.72 AAGIRR68 pKa = 11.84 RR69 pKa = 11.84 MRR71 pKa = 11.84 GTDD74 pKa = 2.77 GRR76 pKa = 11.84 GSHH79 pKa = 5.71 RR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 11.84 RR83 pKa = 11.84 FFF85 pKa = 4.33
Molecular weight: 10.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.41
IPC2_protein 10.979
IPC_protein 12.471
Toseland 12.632
ProMoST 13.13
Dawson 12.632
Bjellqvist 12.632
Wikipedia 13.115
Rodwell 12.223
Grimsley 12.676
Solomon 13.13
Lehninger 13.027
Nozaki 12.632
DTASelect 12.632
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 11.945
IPC_peptide 13.13
IPC2_peptide 12.12
IPC2.peptide.svr19 9.123
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12383
6
12389
5835099
44
20891
471.0
52.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.677 ± 0.021
1.295 ± 0.01
5.759 ± 0.015
6.091 ± 0.022
3.822 ± 0.013
6.795 ± 0.021
2.365 ± 0.01
5.287 ± 0.017
4.905 ± 0.02
9.087 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.227 ± 0.008
3.77 ± 0.012
5.711 ± 0.021
4.042 ± 0.014
5.857 ± 0.02
8.085 ± 0.025
5.775 ± 0.017
6.094 ± 0.017
1.531 ± 0.008
2.825 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here