Idiomarina xiamenensis 10-D-4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Idiomarinaceae; Idiomarina; Idiomarina xiamenensis

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K2K287|K2K287_9GAMM LysR family transcriptional regulator OS=Idiomarina xiamenensis 10-D-4 OX=740709 GN=A10D4_11304 PE=3 SV=1
MM1 pKa = 7.9EE2 pKa = 5.15YY3 pKa = 8.87NTSEE7 pKa = 4.54LCDD10 pKa = 4.82LYY12 pKa = 10.59PDD14 pKa = 3.51MVDD17 pKa = 3.0VVEE20 pKa = 4.72PMFVHH25 pKa = 5.88YY26 pKa = 10.27GGRR29 pKa = 11.84SSFGGQLVTIKK40 pKa = 10.62CFEE43 pKa = 4.62DD44 pKa = 3.05KK45 pKa = 11.24GLIEE49 pKa = 4.85EE50 pKa = 4.98LVQQAGTGKK59 pKa = 10.21VLLIDD64 pKa = 4.05GGGSSRR70 pKa = 11.84RR71 pKa = 11.84ALIDD75 pKa = 3.06IGIAEE80 pKa = 4.22QALDD84 pKa = 4.19NDD86 pKa = 4.1WEE88 pKa = 4.63GIICYY93 pKa = 10.04GAVRR97 pKa = 11.84DD98 pKa = 3.98VDD100 pKa = 4.92ALDD103 pKa = 4.17EE104 pKa = 4.66LDD106 pKa = 4.12LGIMALAAIPVGADD120 pKa = 2.95NAGVGEE126 pKa = 4.33ADD128 pKa = 3.13IAVNFAGVTFLPEE141 pKa = 3.6DD142 pKa = 3.4HH143 pKa = 7.21VYY145 pKa = 11.28ADD147 pKa = 3.21STGIVLSAEE156 pKa = 4.14PLDD159 pKa = 4.08IEE161 pKa = 4.55

Molecular weight: 17.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K2K8L1|K2K8L1_9GAMM Uncharacterized protein OS=Idiomarina xiamenensis 10-D-4 OX=740709 GN=A10D4_08734 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.93VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2673

0

2673

878315

20

7808

328.6

36.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.303 ± 0.061

0.924 ± 0.014

5.852 ± 0.04

5.516 ± 0.043

3.653 ± 0.034

6.737 ± 0.046

2.242 ± 0.022

5.382 ± 0.035

3.722 ± 0.039

10.99 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.352 ± 0.023

3.594 ± 0.036

4.013 ± 0.025

6.47 ± 0.07

5.818 ± 0.035

6.287 ± 0.039

4.802 ± 0.028

6.935 ± 0.035

1.398 ± 0.021

3.012 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski