Batrachochytrium dendrobatidis (strain JEL423)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Chytridiomycota; Chytridiomycota incertae sedis; Chytridiomycetes; Rhizophydiales; Rhizophydiales incertae sedis; Batrachochytrium; Batrachochytrium dendrobatidis

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9531 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177X0J6|A0A177X0J6_BATDL BAG domain-containing protein OS=Batrachochytrium dendrobatidis (strain JEL423) OX=403673 GN=BDEG_28441 PE=4 SV=1
MM1 pKa = 7.42HH2 pKa = 7.85PSIQCQVDD10 pKa = 3.63TTSITKK16 pKa = 10.4NYY18 pKa = 9.28TSDD21 pKa = 3.64NIKK24 pKa = 10.85YY25 pKa = 9.71PDD27 pKa = 5.87DD28 pKa = 3.47IAQLDD33 pKa = 4.16DD34 pKa = 4.76EE35 pKa = 5.77LDD37 pKa = 3.82EE38 pKa = 4.47QDD40 pKa = 5.38SLFPDD45 pKa = 3.44ITILFNNTSYY55 pKa = 11.35PLFSTTAALVPDD67 pKa = 4.81DD68 pKa = 5.0YY69 pKa = 11.59IPFSFDD75 pKa = 3.86LDD77 pKa = 4.17EE78 pKa = 5.56EE79 pKa = 4.52DD80 pKa = 4.8LPSNVLLNSPLYY92 pKa = 10.17YY93 pKa = 10.47LVRR96 pKa = 11.84EE97 pKa = 4.46LKK99 pKa = 10.39HH100 pKa = 5.13VLNIKK105 pKa = 10.29SEE107 pKa = 4.17LYY109 pKa = 10.34DD110 pKa = 3.34VSIQIPQLDD119 pKa = 5.14LYY121 pKa = 11.52AMHH124 pKa = 7.06QDD126 pKa = 3.62LPILNRR132 pKa = 11.84LTLPTLIEE140 pKa = 3.89FHH142 pKa = 5.74QARR145 pKa = 11.84HH146 pKa = 4.7FQSVGSDD153 pKa = 3.18TASALPSLEE162 pKa = 4.82IILIQHH168 pKa = 5.77PTSLKK173 pKa = 9.78HH174 pKa = 6.0QLSLLEE180 pKa = 4.12QICTEE185 pKa = 4.47GGLDD189 pKa = 3.68SNPIVLDD196 pKa = 3.76SDD198 pKa = 4.03DD199 pKa = 5.66DD200 pKa = 4.21NLGDD204 pKa = 3.66EE205 pKa = 4.8YY206 pKa = 11.38EE207 pKa = 4.35NNTLALSTPIQPVSKK222 pKa = 10.41DD223 pKa = 3.09SHH225 pKa = 4.93FQKK228 pKa = 10.74NAAEE232 pKa = 4.36IDD234 pKa = 3.54IKK236 pKa = 11.32DD237 pKa = 3.97DD238 pKa = 3.99LLILGDD244 pKa = 4.35HH245 pKa = 6.45SLHH248 pKa = 6.97SDD250 pKa = 3.28GNLEE254 pKa = 4.04HH255 pKa = 6.53TASSVFTISTPGRR268 pKa = 11.84NHH270 pKa = 6.52TNTVVDD276 pKa = 3.57QTTEE280 pKa = 4.1DD281 pKa = 3.66EE282 pKa = 4.74NDD284 pKa = 3.36NNDD287 pKa = 5.36CIEE290 pKa = 3.82QDD292 pKa = 3.42NPKK295 pKa = 8.59TQIEE299 pKa = 4.36QTLSLNDD306 pKa = 3.35NHH308 pKa = 6.28SACLVTQISDD318 pKa = 3.58PTVEE322 pKa = 4.64AFISTPHH329 pKa = 5.94QPLSADD335 pKa = 3.08SSNPISTTEE344 pKa = 3.86CDD346 pKa = 3.39TTQVLPTDD354 pKa = 4.07SDD356 pKa = 4.55LVIQPVGIDD365 pKa = 3.32DD366 pKa = 4.22HH367 pKa = 8.79ASEE370 pKa = 5.54DD371 pKa = 3.87VLADD375 pKa = 3.33ASMIDD380 pKa = 4.47PEE382 pKa = 4.49ILDD385 pKa = 4.2AFDD388 pKa = 4.2SEE390 pKa = 4.89VVNDD394 pKa = 3.46EE395 pKa = 4.63MYY397 pKa = 11.23AEE399 pKa = 5.92DD400 pKa = 3.92IDD402 pKa = 4.46NDD404 pKa = 3.38EE405 pKa = 5.04TYY407 pKa = 10.66TEE409 pKa = 5.69DD410 pKa = 3.15IGNDD414 pKa = 2.86EE415 pKa = 4.82TYY417 pKa = 11.25AEE419 pKa = 5.29DD420 pKa = 3.26IGNDD424 pKa = 2.97EE425 pKa = 4.8TYY427 pKa = 11.25AEE429 pKa = 4.04DD430 pKa = 3.51TGNDD434 pKa = 3.17EE435 pKa = 6.03HH436 pKa = 8.66IDD438 pKa = 3.57ADD440 pKa = 3.83GSIAFTTFVSDD451 pKa = 3.53NTLQDD456 pKa = 2.74VDD458 pKa = 4.11STVLEE463 pKa = 3.95NTEE466 pKa = 3.76AHH468 pKa = 6.31FEE470 pKa = 4.42DD471 pKa = 5.05GFVAEE476 pKa = 4.25EE477 pKa = 4.17FEE479 pKa = 4.58YY480 pKa = 10.47TVQGDD485 pKa = 3.87EE486 pKa = 4.4STDD489 pKa = 3.47QYY491 pKa = 11.66SSNINDD497 pKa = 3.69DD498 pKa = 3.82TIDD501 pKa = 3.92AVDD504 pKa = 3.47STLIIDD510 pKa = 4.44LDD512 pKa = 3.77VAKK515 pKa = 10.87VVDD518 pKa = 3.81HH519 pKa = 6.65QGDD522 pKa = 3.48EE523 pKa = 4.4GTDD526 pKa = 3.39QVFDD530 pKa = 4.26DD531 pKa = 4.45PLVEE535 pKa = 4.25VEE537 pKa = 4.89LDD539 pKa = 3.62NMDD542 pKa = 4.88ALMDD546 pKa = 4.29DD547 pKa = 3.67MEE549 pKa = 4.5YY550 pKa = 10.41TEE552 pKa = 5.8EE553 pKa = 4.29IDD555 pKa = 3.58PHH557 pKa = 7.68VDD559 pKa = 3.39EE560 pKa = 5.72NDD562 pKa = 3.09VALDD566 pKa = 3.35VDD568 pKa = 4.15TDD570 pKa = 3.86HH571 pKa = 7.66QSLQNTDD578 pKa = 3.28QAKK581 pKa = 10.57DD582 pKa = 3.33SADD585 pKa = 3.12AHH587 pKa = 6.37ILLEE591 pKa = 4.48KK592 pKa = 9.87ATADD596 pKa = 4.43AIHH599 pKa = 6.92DD600 pKa = 3.83TALDD604 pKa = 3.89AVCIDD609 pKa = 3.89PTNSNLDD616 pKa = 3.71SEE618 pKa = 5.11VDD620 pKa = 3.52DD621 pKa = 4.31LNAYY625 pKa = 9.31KK626 pKa = 10.54FSLGSKK632 pKa = 9.91RR633 pKa = 11.84EE634 pKa = 4.07LEE636 pKa = 4.19QEE638 pKa = 4.16HH639 pKa = 7.52DD640 pKa = 3.49DD641 pKa = 4.02AAAKK645 pKa = 9.81RR646 pKa = 11.84ARR648 pKa = 11.84VV649 pKa = 3.34

Molecular weight: 71.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177WM73|A0A177WM73_BATDL Uncharacterized protein OS=Batrachochytrium dendrobatidis (strain JEL423) OX=403673 GN=BDEG_24852 PE=3 SV=1
MM1 pKa = 7.07PHH3 pKa = 6.59SRR5 pKa = 11.84LLCVFSSNAFKK16 pKa = 11.05LPLPNVRR23 pKa = 11.84EE24 pKa = 4.27KK25 pKa = 10.69IVLPLIRR32 pKa = 11.84LQHH35 pKa = 6.59PPLQPALDD43 pKa = 3.77LTALQRR49 pKa = 11.84KK50 pKa = 7.82LPWMLTMRR58 pKa = 11.84RR59 pKa = 11.84IQMPMVKK66 pKa = 9.23ISKK69 pKa = 8.34MNSMNLMTKK78 pKa = 9.9ISRR81 pKa = 11.84KK82 pKa = 8.18GTSLPKK88 pKa = 9.82PLPPRR93 pKa = 11.84MTIKK97 pKa = 9.52STRR100 pKa = 11.84IPSLLPRR107 pKa = 11.84VKK109 pKa = 9.52STRR112 pKa = 11.84IPNPLPRR119 pKa = 11.84LEE121 pKa = 4.16VKK123 pKa = 9.64RR124 pKa = 11.84RR125 pKa = 11.84KK126 pKa = 9.69RR127 pKa = 11.84MRR129 pKa = 11.84RR130 pKa = 11.84KK131 pKa = 7.03MRR133 pKa = 11.84KK134 pKa = 8.06RR135 pKa = 11.84IRR137 pKa = 11.84SIRR140 pKa = 11.84IPSLLPSSPRR150 pKa = 11.84LQLLPRR156 pKa = 11.84PLPRR160 pKa = 11.84PQLLPRR166 pKa = 11.84LLLLPRR172 pKa = 11.84PQLPPRR178 pKa = 11.84PLQRR182 pKa = 11.84PQPRR186 pKa = 11.84PLLQPLPLQVKK197 pKa = 9.89ASTLRR202 pKa = 11.84RR203 pKa = 11.84MM204 pKa = 3.95

Molecular weight: 23.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8580

951

9531

4695730

29

4754

492.7

54.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.013 ± 0.022

1.47 ± 0.01

5.698 ± 0.017

5.392 ± 0.024

3.818 ± 0.014

5.086 ± 0.022

2.748 ± 0.013

6.163 ± 0.016

5.701 ± 0.026

9.258 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.437 ± 0.009

4.609 ± 0.014

4.988 ± 0.026

4.742 ± 0.02

4.759 ± 0.017

9.765 ± 0.031

6.558 ± 0.02

5.98 ± 0.018

1.005 ± 0.007

2.813 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski