Myocastor coypus polyomavirus 1
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346RRK3|A0A346RRK3_9POLY Minor capsid protein VP2 OS=Myocastor coypus polyomavirus 1 OX=2304056 PE=3 SV=1
MM1 pKa = 7.19 GAVLAVIAEE10 pKa = 4.2 VFGFEE15 pKa = 4.07 LAAAGTAGATATALAEE31 pKa = 4.53 LGTEE35 pKa = 4.17 TVLTGEE41 pKa = 4.84 LVTSLEE47 pKa = 4.13 SQIASIMAIEE57 pKa = 4.98 GVTQTEE63 pKa = 3.87 ALAALGISAEE73 pKa = 4.35 SYY75 pKa = 10.29 GALTEE80 pKa = 4.72 AYY82 pKa = 10.01 LAGVDD87 pKa = 4.6 ALGTQIGSGVSTGLGQTLIQGLSNINVPIGPFFQTITGTSYY128 pKa = 11.14 LVAAGIQFHH137 pKa = 6.06 KK138 pKa = 10.31 HH139 pKa = 3.73 QVSIVNRR146 pKa = 11.84 NNMALVPYY154 pKa = 10.05 IPEE157 pKa = 4.57 DD158 pKa = 3.77 YY159 pKa = 11.11 YY160 pKa = 11.44 DD161 pKa = 3.39 ILFPGVQSFTRR172 pKa = 11.84 GLNVVEE178 pKa = 4.75 NWATSLFRR186 pKa = 11.84 SLGHH190 pKa = 6.31 YY191 pKa = 9.68 LFQQLVRR198 pKa = 11.84 EE199 pKa = 4.29 AHH201 pKa = 5.48 AQIGQAGRR209 pKa = 11.84 SAGNALISYY218 pKa = 7.01 GTRR221 pKa = 11.84 GLEE224 pKa = 3.62 EE225 pKa = 3.88 SVARR229 pKa = 11.84 FLEE232 pKa = 4.2 SARR235 pKa = 11.84 WVIQGPTNTYY245 pKa = 10.13 NFLRR249 pKa = 11.84 QYY251 pKa = 10.97 YY252 pKa = 10.37 EE253 pKa = 3.77 NLTPLNPPRR262 pKa = 11.84 FVQLEE267 pKa = 3.78 KK268 pKa = 10.69 RR269 pKa = 11.84 LRR271 pKa = 11.84 YY272 pKa = 9.52 LDD274 pKa = 3.79 QVPKK278 pKa = 10.15 RR279 pKa = 11.84 QAPGEE284 pKa = 4.13 SGEE287 pKa = 4.26 IVDD290 pKa = 4.66 TVSSPGGALQRR301 pKa = 11.84 HH302 pKa = 5.33 TPDD305 pKa = 2.44 WMLPLILGLYY315 pKa = 10.31 GDD317 pKa = 5.09 ISPTWRR323 pKa = 11.84 TYY325 pKa = 10.35 IEE327 pKa = 4.19 EE328 pKa = 4.18 EE329 pKa = 4.15 EE330 pKa = 4.21 QEE332 pKa = 4.15 EE333 pKa = 4.95 EE334 pKa = 4.13 YY335 pKa = 11.17 GPKK338 pKa = 9.86 KK339 pKa = 10.41 KK340 pKa = 9.95 RR341 pKa = 11.84 VRR343 pKa = 11.84 YY344 pKa = 9.27 DD345 pKa = 2.82
Molecular weight: 37.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.86
IPC2_protein 4.965
IPC_protein 4.863
Toseland 4.774
ProMoST 4.889
Dawson 4.813
Bjellqvist 4.965
Wikipedia 4.635
Rodwell 4.736
Grimsley 4.698
Solomon 4.813
Lehninger 4.762
Nozaki 4.914
DTASelect 4.991
Thurlkill 4.762
EMBOSS 4.66
Sillero 5.003
Patrickios 3.656
IPC_peptide 4.825
IPC2_peptide 5.003
IPC2.peptide.svr19 4.935
Protein with the highest isoelectric point:
>tr|A0A346RRK5|A0A346RRK5_9POLY Large T antigen OS=Myocastor coypus polyomavirus 1 OX=2304056 PE=4 SV=1
MM1 pKa = 7.84 DD2 pKa = 5.23 RR3 pKa = 11.84 VLGWGEE9 pKa = 4.25 RR10 pKa = 11.84 KK11 pKa = 9.78 RR12 pKa = 11.84 LMHH15 pKa = 6.82 LLGVPLEE22 pKa = 4.47 KK23 pKa = 10.81 YY24 pKa = 10.51 GNYY27 pKa = 9.94 PVMRR31 pKa = 11.84 DD32 pKa = 3.31 AYY34 pKa = 9.01 KK35 pKa = 10.81 SKK37 pKa = 10.25 VLEE40 pKa = 3.9 YY41 pKa = 10.65 HH42 pKa = 6.81 PDD44 pKa = 3.22 KK45 pKa = 11.63 GGDD48 pKa = 3.39 GKK50 pKa = 11.49 LMSEE54 pKa = 5.6 LNNLWKK60 pKa = 10.8 AFTDD64 pKa = 4.07 GLHH67 pKa = 7.09 LLRR70 pKa = 11.84 EE71 pKa = 4.36 CEE73 pKa = 4.12 EE74 pKa = 4.19 VQKK77 pKa = 10.85 QGYY80 pKa = 7.59 TDD82 pKa = 4.71 DD83 pKa = 4.15 DD84 pKa = 4.18 TVKK87 pKa = 10.73 LFLGTPIKK95 pKa = 10.58 GVYY98 pKa = 9.43 CKK100 pKa = 10.59 VYY102 pKa = 9.72 PLCKK106 pKa = 10.31 DD107 pKa = 3.83 FLHH110 pKa = 6.69 KK111 pKa = 10.21 KK112 pKa = 8.7 CKK114 pKa = 10.4 CIICVLKK121 pKa = 10.14 RR122 pKa = 11.84 QHH124 pKa = 6.13 YY125 pKa = 9.05 RR126 pKa = 11.84 MKK128 pKa = 10.93 ARR130 pKa = 11.84 TLKK133 pKa = 10.63 LCLMWGQCYY142 pKa = 10.03 CYY144 pKa = 9.99 HH145 pKa = 7.74 CYY147 pKa = 10.28 LQWFGEE153 pKa = 4.0 DD154 pKa = 3.97 DD155 pKa = 3.9 SYY157 pKa = 11.78 DD158 pKa = 2.96 ICKK161 pKa = 10.23 RR162 pKa = 11.84 YY163 pKa = 10.52 LEE165 pKa = 4.79 TIILMPINLIIYY177 pKa = 9.73 DD178 pKa = 4.21 SIFEE182 pKa = 4.09 CEE184 pKa = 3.94 EE185 pKa = 3.54 WSKK188 pKa = 11.44 YY189 pKa = 10.48 LISLRR194 pKa = 11.84 SIDD197 pKa = 4.66 LYY199 pKa = 10.82 ISLKK203 pKa = 9.8 VLCDD207 pKa = 3.41 CLDD210 pKa = 3.71 RR211 pKa = 11.84 KK212 pKa = 8.83 QTVAEE217 pKa = 4.35 APHH220 pKa = 6.23 MEE222 pKa = 4.54 LKK224 pKa = 10.54 PSEE227 pKa = 4.17 EE228 pKa = 4.39 HH229 pKa = 6.8 GMQLGEE235 pKa = 4.77 LEE237 pKa = 4.3 ALQARR242 pKa = 11.84 LNPTPTQTSFVMNPWVSPPLMTSTPRR268 pKa = 11.84 LNRR271 pKa = 11.84 HH272 pKa = 5.58 LHH274 pKa = 5.71 LAPPRR279 pKa = 11.84 GEE281 pKa = 4.37 EE282 pKa = 4.03 GDD284 pKa = 3.65 KK285 pKa = 11.11 ARR287 pKa = 11.84 SNPTPTKK294 pKa = 9.88 TFFGLTPGGQPPCC307 pKa = 4.38
Molecular weight: 35.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.076
IPC2_protein 7.293
IPC_protein 7.117
Toseland 6.781
ProMoST 7.761
Dawson 7.819
Bjellqvist 8.273
Wikipedia 7.732
Rodwell 7.834
Grimsley 6.825
Solomon 7.863
Lehninger 7.878
Nozaki 8.551
DTASelect 7.98
Thurlkill 8.024
EMBOSS 8.039
Sillero 8.375
Patrickios 3.808
IPC_peptide 7.849
IPC2_peptide 7.468
IPC2.peptide.svr19 7.352
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1857
197
625
371.4
41.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.331 ± 1.143
2.423 ± 0.889
4.362 ± 0.604
7.701 ± 0.342
3.446 ± 0.365
7.485 ± 1.026
2.154 ± 0.465
5.17 ± 0.29
6.354 ± 1.488
10.232 ± 1.013
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.477 ± 0.4
3.823 ± 0.415
5.924 ± 0.717
4.47 ± 0.432
5.116 ± 0.507
5.6 ± 0.373
5.87 ± 0.771
6.462 ± 0.601
1.4 ± 0.192
4.2 ± 0.608
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here