Candidatus Regiella insecticola 5.15

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; aphid secondary symbionts; Candidatus Regiella; Candidatus Regiella insecticola

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2GZE8|G2GZE8_9ENTR Superfamily II DNA and RNA helicase OS=Candidatus Regiella insecticola 5.15 OX=1005043 GN=Rin_00011630 PE=4 SV=1
AA1 pKa = 7.79AEE3 pKa = 3.99LVSYY7 pKa = 10.29YY8 pKa = 11.41NNNTGNNNANDD19 pKa = 4.01EE20 pKa = 4.31EE21 pKa = 4.51AVIPMPHH28 pKa = 7.45LLPSII33 pKa = 4.35

Molecular weight: 3.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2GWM7|G2GWM7_9ENTR Uncharacterized protein OS=Candidatus Regiella insecticola 5.15 OX=1005043 GN=Rin_00001630 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22SRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2269

0

2269

540769

20

2714

238.3

26.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.301 ± 0.057

1.14 ± 0.023

5.126 ± 0.05

5.688 ± 0.058

3.814 ± 0.039

6.404 ± 0.062

2.432 ± 0.026

7.036 ± 0.048

5.809 ± 0.049

10.844 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.495 ± 0.028

4.631 ± 0.044

4.029 ± 0.034

4.978 ± 0.053

5.34 ± 0.046

6.298 ± 0.041

5.417 ± 0.036

6.049 ± 0.051

1.191 ± 0.023

2.974 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski