SAR86 cluster bacterium SAR86B

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; SAR86 cluster

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1807 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J4WX98|J4WX98_9GAMM Uncharacterized protein OS=SAR86 cluster bacterium SAR86B OX=1123867 GN=NT02SARS_1192 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.29SKK4 pKa = 10.89SSLFIAFLFLFSCGGGGGGGSAALPLPIITIAASSLDD41 pKa = 3.82VNIGDD46 pKa = 4.94DD47 pKa = 4.75VDD49 pKa = 4.06ITWSVSNSNSCNATGAWSGIKK70 pKa = 10.19SNSGNEE76 pKa = 4.05TVTVSGAGNNNFGLSCSGEE95 pKa = 4.04GGQASEE101 pKa = 4.46TVTVFAFNLAANNTTLAVDD120 pKa = 3.82EE121 pKa = 5.47DD122 pKa = 4.29GSLINSSVAVQPNTDD137 pKa = 2.99ILVSYY142 pKa = 10.36SLLSSTSNGNLTFNEE157 pKa = 4.39DD158 pKa = 2.91ATINYY163 pKa = 9.29YY164 pKa = 10.0PDD166 pKa = 3.85PNFNGVDD173 pKa = 3.25EE174 pKa = 4.84FTFKK178 pKa = 10.96AIAATRR184 pKa = 11.84SVEE187 pKa = 3.83KK188 pKa = 10.8DD189 pKa = 3.05VTVTINIASINDD201 pKa = 3.57APTIALDD208 pKa = 4.02SEE210 pKa = 5.11SNLSKK215 pKa = 11.0LDD217 pKa = 3.43MVYY220 pKa = 10.72EE221 pKa = 4.18SDD223 pKa = 4.94PIFSFNFADD232 pKa = 3.72VDD234 pKa = 3.88HH235 pKa = 6.95LLEE238 pKa = 5.19DD239 pKa = 3.75LTFTASVDD247 pKa = 3.54GNAVPTTFNQISEE260 pKa = 5.0GYY262 pKa = 10.65GSLQLDD268 pKa = 4.5LSSLGSGGLFDD279 pKa = 4.26LTLSVFDD286 pKa = 4.76GFDD289 pKa = 3.25TATEE293 pKa = 4.12TLNTWFVADD302 pKa = 4.0KK303 pKa = 10.47STVTFDD309 pKa = 4.43QDD311 pKa = 3.93DD312 pKa = 4.09DD313 pKa = 4.88PEE315 pKa = 6.54DD316 pKa = 4.0GVTEE320 pKa = 4.55GSSTSVDD327 pKa = 3.35YY328 pKa = 11.07NVYY331 pKa = 10.24YY332 pKa = 11.02LDD334 pKa = 4.42GNSEE338 pKa = 4.08SRR340 pKa = 11.84GRR342 pKa = 11.84TTYY345 pKa = 11.25LFVADD350 pKa = 4.23SLASEE355 pKa = 4.47EE356 pKa = 4.34DD357 pKa = 3.33RR358 pKa = 11.84ASFRR362 pKa = 11.84SALVRR367 pKa = 11.84SINKK371 pKa = 9.25VKK373 pKa = 10.63EE374 pKa = 3.78SDD376 pKa = 3.1AGEE379 pKa = 4.58FITGFFTIKK388 pKa = 10.16AAEE391 pKa = 3.92PVIPDD396 pKa = 3.91GKK398 pKa = 10.33SPSAIRR404 pKa = 11.84TGCYY408 pKa = 10.39DD409 pKa = 4.32FDD411 pKa = 4.97PNIYY415 pKa = 10.28CIGDD419 pKa = 3.38MDD421 pKa = 4.12TSVFDD426 pKa = 3.46VMYY429 pKa = 9.65PGHH432 pKa = 7.12LLVSTLTMQSGRR444 pKa = 11.84GVNLGNRR451 pKa = 11.84NIQPISSRR459 pKa = 11.84TQNVLMHH466 pKa = 6.52EE467 pKa = 4.86LGHH470 pKa = 6.39AHH472 pKa = 6.91GFMGDD477 pKa = 3.69EE478 pKa = 4.24YY479 pKa = 11.11RR480 pKa = 11.84SDD482 pKa = 3.9DD483 pKa = 4.79DD484 pKa = 5.68RR485 pKa = 11.84DD486 pKa = 3.32VSYY489 pKa = 9.81WADD492 pKa = 3.47LNINTTTQSDD502 pKa = 3.81PALVKK507 pKa = 9.65WKK509 pKa = 10.26HH510 pKa = 6.18LISDD514 pKa = 4.5PLNVLGQDD522 pKa = 3.3IQVCYY527 pKa = 10.06NWEE530 pKa = 4.55DD531 pKa = 3.51GTIADD536 pKa = 4.7FDD538 pKa = 5.33DD539 pKa = 3.89IGIVVEE545 pKa = 5.53DD546 pKa = 4.54CDD548 pKa = 4.66CFINIWDD555 pKa = 3.85EE556 pKa = 4.0NGNFLGKK563 pKa = 10.09NPEE566 pKa = 4.04CSEE569 pKa = 4.02VGLFEE574 pKa = 4.43GNYY577 pKa = 9.66YY578 pKa = 10.92GLYY581 pKa = 10.56DD582 pKa = 3.47NYY584 pKa = 10.97RR585 pKa = 11.84PTFCSIMDD593 pKa = 3.67SCSAAGYY600 pKa = 9.62QKK602 pKa = 11.13VNAEE606 pKa = 3.98GFAVGSIHH614 pKa = 6.69NQGFYY619 pKa = 11.1NSDD622 pKa = 3.22SVGFLTDD629 pKa = 3.36STTGEE634 pKa = 4.07YY635 pKa = 10.24TDD637 pKa = 4.28FEE639 pKa = 4.29IVIDD643 pKa = 4.45GEE645 pKa = 4.54LDD647 pKa = 3.25TSKK650 pKa = 11.0LVLKK654 pKa = 9.68WYY656 pKa = 9.98VNGVEE661 pKa = 4.33EE662 pKa = 4.52VGLRR666 pKa = 11.84NQLGAIFPRR675 pKa = 11.84PANNAIEE682 pKa = 4.41IYY684 pKa = 9.26TYY686 pKa = 10.54RR687 pKa = 11.84ITDD690 pKa = 3.58LTGTITAPDD699 pKa = 3.49QVLVYY704 pKa = 10.99DD705 pKa = 4.67DD706 pKa = 4.88FYY708 pKa = 11.71EE709 pKa = 4.57GLLNSDD715 pKa = 4.42FQWXXXXXXXLSTAYY730 pKa = 10.32DD731 pKa = 3.89YY732 pKa = 11.65AWCCNKK738 pKa = 9.94SRR740 pKa = 11.84IDD742 pKa = 3.57PNGEE746 pKa = 3.5II747 pKa = 4.56

Molecular weight: 80.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J5KLI8|J5KLI8_9GAMM Glucose-inhibited division protein A OS=SAR86 cluster bacterium SAR86B OX=1123867 GN=gidA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.2AGRR28 pKa = 11.84AILANRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 10.03KK37 pKa = 9.78GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1807

0

1807

523114

29

1477

289.5

32.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.17 ± 0.059

0.919 ± 0.017

6.007 ± 0.045

6.54 ± 0.051

4.861 ± 0.049

6.439 ± 0.057

1.692 ± 0.024

9.006 ± 0.075

7.503 ± 0.074

9.345 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.182 ± 0.026

6.414 ± 0.056

3.47 ± 0.035

2.819 ± 0.026

3.418 ± 0.04

7.594 ± 0.051

4.769 ± 0.04

5.727 ± 0.053

0.998 ± 0.024

3.549 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski