Hordeum vulgare subsp. vulgare (Domesticated barley)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade;

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 189799 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M0V9N4|M0V9N4_HORVV Isoform of A0A287PHC5 Uncharacterized protein OS=Hordeum vulgare subsp. vulgare OX=112509 PE=4 SV=1
MM1 pKa = 7.29HH2 pKa = 6.18VVCRR6 pKa = 11.84IFQKK10 pKa = 10.73VGSGPQNGAQYY21 pKa = 9.57GAPYY25 pKa = 9.48MEE27 pKa = 5.18EE28 pKa = 3.88EE29 pKa = 4.76WEE31 pKa = 4.61DD32 pKa = 3.53EE33 pKa = 4.22DD34 pKa = 5.85DD35 pKa = 5.58AIEE38 pKa = 4.09NTPTSGTSTEE48 pKa = 4.18MLAITDD54 pKa = 3.86TASAEE59 pKa = 4.47SNVEE63 pKa = 3.87DD64 pKa = 3.95EE65 pKa = 5.29NIFSGINEE73 pKa = 4.49LVQIQDD79 pKa = 3.35VLIPPEE85 pKa = 4.01IVAPIQAIAPLQAQGLNGTGMGSYY109 pKa = 11.21ADD111 pKa = 4.04GDD113 pKa = 3.9VSLDD117 pKa = 3.98EE118 pKa = 4.6ILQEE122 pKa = 4.05PVSDD126 pKa = 3.64VSVDD130 pKa = 3.41NTGEE134 pKa = 4.11PEE136 pKa = 4.16EE137 pKa = 4.08QSPIDD142 pKa = 3.77DD143 pKa = 4.35HH144 pKa = 7.45FSLADD149 pKa = 4.01LSGCPNQDD157 pKa = 3.0DD158 pKa = 5.21GYY160 pKa = 10.97VRR162 pKa = 11.84QAGLTMWSDD171 pKa = 3.82PSNGDD176 pKa = 3.02QAYY179 pKa = 9.14YY180 pKa = 10.06PIIRR184 pKa = 11.84TYY186 pKa = 11.28GNRR189 pKa = 11.84NHH191 pKa = 6.96ANMALSDD198 pKa = 3.79AEE200 pKa = 4.38YY201 pKa = 10.7FDD203 pKa = 5.01TEE205 pKa = 4.26NDD207 pKa = 3.16TNAYY211 pKa = 9.88FGQQQACPSDD221 pKa = 3.66DD222 pKa = 3.5QNLYY226 pKa = 10.66SGQQQACPSDD236 pKa = 3.67DD237 pKa = 3.54QNLYY241 pKa = 10.79GPSFPQQVGDD251 pKa = 3.53NEE253 pKa = 4.2QFYY256 pKa = 10.32EE257 pKa = 4.49ASSDD261 pKa = 3.79HH262 pKa = 6.38KK263 pKa = 10.72WVDD266 pKa = 4.1GKK268 pKa = 10.75DD269 pKa = 3.2DD270 pKa = 3.87CANVNDD276 pKa = 5.35LSIFDD281 pKa = 4.88DD282 pKa = 6.18DD283 pKa = 5.87IMSLLNASEE292 pKa = 4.64GDD294 pKa = 3.56FSLDD298 pKa = 3.31LLGPVDD304 pKa = 4.16GSNSQFPAASDD315 pKa = 3.86FDD317 pKa = 4.03QKK319 pKa = 11.66DD320 pKa = 3.38EE321 pKa = 4.72AKK323 pKa = 9.97AQYY326 pKa = 9.62GASSSGSRR334 pKa = 11.84EE335 pKa = 3.72NLYY338 pKa = 9.71PDD340 pKa = 4.64SMVTDD345 pKa = 4.67LPMDD349 pKa = 4.64DD350 pKa = 4.46NVGKK354 pKa = 10.83RR355 pKa = 11.84FGKK358 pKa = 8.89FANMLGSYY366 pKa = 7.88PAPPAMASEE375 pKa = 4.84FPPTTGKK382 pKa = 10.59SIAALSGPSQIRR394 pKa = 11.84VTAGIVQLGDD404 pKa = 3.2HH405 pKa = 6.59SFTGNSDD412 pKa = 2.93NWPLQKK418 pKa = 10.88NGVFNLLLSFTVEE431 pKa = 4.5SNVSTKK437 pKa = 10.88LIGFDD442 pKa = 4.87DD443 pKa = 4.69EE444 pKa = 4.57PATTRR449 pKa = 11.84VSTVPTVLRR458 pKa = 11.84GGLYY462 pKa = 10.61LFFVSAMILMLSFKK476 pKa = 10.49VGSCIYY482 pKa = 10.71SRR484 pKa = 3.98

Molecular weight: 52.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A287JF95|A0A287JF95_HORVV Isoform of A0A287JEV7 Mediator of RNA polymerase II transcription subunit 13 OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1
PP1 pKa = 7.93PIPTPQRR8 pKa = 11.84HH9 pKa = 5.15RR10 pKa = 11.84PPWLSLTPASTPRR23 pKa = 11.84APPLSAPPPPPLAPPRR39 pKa = 11.84VPRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84PPSPPPPSPALTSPPRR62 pKa = 11.84APRR65 pKa = 11.84PVAPRR70 pKa = 11.84PSAPPWAPRR79 pKa = 11.84HH80 pKa = 5.45PRR82 pKa = 11.84ISSGPPPAA90 pKa = 5.69

Molecular weight: 9.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35965

153834

189799

68226991

29

5383

359.5

39.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.23 ± 0.007

1.932 ± 0.003

5.36 ± 0.004

6.006 ± 0.007

3.787 ± 0.004

6.943 ± 0.006

2.554 ± 0.003

4.71 ± 0.005

5.188 ± 0.005

9.711 ± 0.007

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.44 ± 0.003

3.683 ± 0.004

5.366 ± 0.006

3.68 ± 0.004

6.04 ± 0.006

8.483 ± 0.006

4.896 ± 0.003

6.687 ± 0.004

1.262 ± 0.002

2.682 ± 0.003
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski