Triticum aestivum (Wheat)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae;

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 130673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B6ARP4|A0A3B6ARP4_WHEAT Uncharacterized protein OS=Triticum aestivum OX=4565 PE=4 SV=1
MM1 pKa = 7.17GKK3 pKa = 10.26AYY5 pKa = 10.28RR6 pKa = 11.84KK7 pKa = 9.97KK8 pKa = 10.87LGVNKK13 pKa = 10.13AATPAGGKK21 pKa = 10.19GKK23 pKa = 10.49GAGGGNAKK31 pKa = 10.23RR32 pKa = 11.84SGGAAGAGGAGPGGGAKK49 pKa = 10.22GAGGAGWGGASTFSATYY66 pKa = 10.04HH67 pKa = 5.68STFQTAVNVTNEE79 pKa = 4.49YY80 pKa = 9.74YY81 pKa = 10.53NQWGNDD87 pKa = 3.44CSSDD91 pKa = 4.14DD92 pKa = 4.92DD93 pKa = 4.9GPPSAPADD101 pKa = 3.64DD102 pKa = 4.42STFEE106 pKa = 4.09TAVNDD111 pKa = 3.7TNEE114 pKa = 4.12YY115 pKa = 8.71YY116 pKa = 10.09QEE118 pKa = 4.49EE119 pKa = 4.32EE120 pKa = 4.83DD121 pKa = 4.48NNCSSPFAPADD132 pKa = 3.21HH133 pKa = 6.51STFEE137 pKa = 4.2TAVKK141 pKa = 10.2IMNEE145 pKa = 4.09HH146 pKa = 6.47YY147 pKa = 10.99DD148 pKa = 3.57DD149 pKa = 5.79DD150 pKa = 4.87EE151 pKa = 5.0NLGLSMDD158 pKa = 3.95SEE160 pKa = 4.72EE161 pKa = 5.36EE162 pKa = 4.38GVQDD166 pKa = 4.93AEE168 pKa = 4.44PVSDD172 pKa = 5.09ADD174 pKa = 5.29GEE176 pKa = 4.4GDD178 pKa = 4.18DD179 pKa = 5.42DD180 pKa = 5.36AGSGDD185 pKa = 4.1DD186 pKa = 5.13HH187 pKa = 8.31FGDD190 pKa = 4.96DD191 pKa = 5.5DD192 pKa = 5.48YY193 pKa = 11.97YY194 pKa = 11.61DD195 pKa = 5.53DD196 pKa = 6.76DD197 pKa = 4.42IAGCFDD203 pKa = 3.62AFYY206 pKa = 10.92DD207 pKa = 4.59PGDD210 pKa = 4.28AGDD213 pKa = 5.56GADD216 pKa = 4.3DD217 pKa = 3.21WWW219 pKa = 5.75

Molecular weight: 22.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B6QD59|A0A3B6QD59_WHEAT Uncharacterized protein OS=Triticum aestivum OX=4565 PE=4 SV=1
MM1 pKa = 7.07YY2 pKa = 10.41LVYY5 pKa = 10.35YY6 pKa = 7.86YY7 pKa = 9.57QHH9 pKa = 7.08IIKK12 pKa = 10.1SCLISTTPLLLLAKK26 pKa = 9.32VHH28 pKa = 6.21IFVLLRR34 pKa = 11.84FSPMHH39 pKa = 5.88IHH41 pKa = 6.17RR42 pKa = 11.84TLPSPCVSSVAFPPHH57 pKa = 6.26SKK59 pKa = 10.13KK60 pKa = 10.6ASRR63 pKa = 11.84PSTAAAHH70 pKa = 5.74HH71 pKa = 6.08RR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84HH76 pKa = 4.64GRR78 pKa = 11.84GHH80 pKa = 6.27GAGHH84 pKa = 7.02GLLLPPAARR93 pKa = 11.84LGRR96 pKa = 11.84HH97 pKa = 6.25LAPPPPPLPRR107 pKa = 11.84RR108 pKa = 11.84APPGPRR114 pKa = 11.84TRR116 pKa = 11.84RR117 pKa = 11.84ARR119 pKa = 11.84PLRR122 pKa = 11.84RR123 pKa = 11.84GRR125 pKa = 11.84PRR127 pKa = 11.84GRR129 pKa = 11.84RR130 pKa = 11.84AGGGTASSAAAGAAPSGGVGEE151 pKa = 4.38AQGAGAPAAGVAGGGGADD169 pKa = 3.12GRR171 pKa = 11.84VRR173 pKa = 11.84RR174 pKa = 11.84GGRR177 pKa = 11.84GGPRR181 pKa = 11.84RR182 pKa = 11.84LRR184 pKa = 11.84RR185 pKa = 11.84RR186 pKa = 11.84RR187 pKa = 11.84VPVQDD192 pKa = 3.31PRR194 pKa = 11.84VGRR197 pKa = 11.84RR198 pKa = 11.84APGRR202 pKa = 11.84RR203 pKa = 11.84EE204 pKa = 3.51GAPLVRR210 pKa = 11.84RR211 pKa = 11.84RR212 pKa = 11.84RR213 pKa = 11.84APARR217 pKa = 11.84RR218 pKa = 11.84VPARR222 pKa = 11.84ARR224 pKa = 11.84PAPPRR229 pKa = 11.84RR230 pKa = 11.84PPPAPRR236 pKa = 11.84RR237 pKa = 11.84PPRR240 pKa = 11.84LLRR243 pKa = 11.84RR244 pKa = 11.84PRR246 pKa = 11.84GGRR249 pKa = 11.84RR250 pKa = 11.84AGAGVHH256 pKa = 6.58AGRR259 pKa = 11.84HH260 pKa = 5.27AGGPSPPRR268 pKa = 11.84RR269 pKa = 11.84HH270 pKa = 5.94AAAHH274 pKa = 6.67LAPPHH279 pKa = 6.5AGPPRR284 pKa = 11.84RR285 pKa = 11.84GRR287 pKa = 11.84RR288 pKa = 11.84AGAPPRR294 pKa = 11.84RR295 pKa = 11.84RR296 pKa = 11.84LHH298 pKa = 5.56RR299 pKa = 11.84ARR301 pKa = 11.84RR302 pKa = 11.84RR303 pKa = 11.84VGLQRR308 pKa = 11.84APRR311 pKa = 11.84RR312 pKa = 11.84RR313 pKa = 11.84RR314 pKa = 11.84PRR316 pKa = 11.84AALRR320 pKa = 11.84PGVGLRR326 pKa = 11.84GHH328 pKa = 7.31LLGGRR333 pKa = 11.84GPRR336 pKa = 11.84PGRR339 pKa = 11.84GGRR342 pKa = 11.84RR343 pKa = 11.84LAGLRR348 pKa = 11.84RR349 pKa = 11.84PLLPPHH355 pKa = 6.15RR356 pKa = 11.84HH357 pKa = 5.08RR358 pKa = 11.84VQEE361 pKa = 3.85VRR363 pKa = 11.84RR364 pKa = 11.84VRR366 pKa = 11.84LRR368 pKa = 11.84RR369 pKa = 11.84AAPRR373 pKa = 11.84GPHH376 pKa = 6.08RR377 pKa = 11.84LAGRR381 pKa = 11.84RR382 pKa = 11.84RR383 pKa = 11.84AGPGRR388 pKa = 11.84RR389 pKa = 11.84RR390 pKa = 11.84RR391 pKa = 11.84PVPRR395 pKa = 11.84GQGAAARR402 pKa = 11.84EE403 pKa = 4.21GRR405 pKa = 11.84RR406 pKa = 11.84GGGARR411 pKa = 11.84GRR413 pKa = 11.84QAGRR417 pKa = 11.84RR418 pKa = 11.84LL419 pKa = 3.31

Molecular weight: 45.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105061

25612

130673

58552452

15

5359

448.1

49.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.805 ± 0.009

1.937 ± 0.003

5.533 ± 0.005

6.069 ± 0.007

3.698 ± 0.004

7.165 ± 0.006

2.51 ± 0.003

4.462 ± 0.005

5.065 ± 0.006

9.714 ± 0.008

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.501 ± 0.003

3.593 ± 0.004

5.459 ± 0.006

3.553 ± 0.005

6.015 ± 0.006

8.301 ± 0.007

4.852 ± 0.004

6.817 ± 0.005

1.288 ± 0.002

2.634 ± 0.003
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski