Hubei sobemo-like virus 38

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEJ2|A0A1L3KEJ2_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 38 OX=1923225 PE=4 SV=1
MM1 pKa = 7.86DD2 pKa = 6.19ASPGLPFCKK11 pKa = 9.71HH12 pKa = 5.33YY13 pKa = 9.37KK14 pKa = 9.39TNAEE18 pKa = 4.09LLGFDD23 pKa = 5.18GFNCDD28 pKa = 4.91AVKK31 pKa = 10.64ADD33 pKa = 3.73CLVNMFYY40 pKa = 11.17YY41 pKa = 10.03WLDD44 pKa = 3.9NIQVYY49 pKa = 8.45PFRR52 pKa = 11.84IFIKK56 pKa = 10.52DD57 pKa = 3.62EE58 pKa = 3.7PHH60 pKa = 7.12KK61 pKa = 10.71LSKK64 pKa = 9.39KK65 pKa = 10.07QKK67 pKa = 9.34GRR69 pKa = 11.84WRR71 pKa = 11.84LIFSSPLFYY80 pKa = 10.73QILEE84 pKa = 4.22HH85 pKa = 7.18LLLDD89 pKa = 4.65PLDD92 pKa = 4.11EE93 pKa = 4.7MEE95 pKa = 6.42SLCQWDD101 pKa = 5.02LPTKK105 pKa = 10.38LRR107 pKa = 11.84WHH109 pKa = 6.74PFWGGAQMGVSNFDD123 pKa = 3.47NPVSLDD129 pKa = 3.57KK130 pKa = 11.31QCWDD134 pKa = 3.09WTLTGQFAEE143 pKa = 4.71LDD145 pKa = 3.35NALRR149 pKa = 11.84SNLVKK154 pKa = 10.74APNKK158 pKa = 7.54WHH160 pKa = 6.57EE161 pKa = 4.35LFEE164 pKa = 4.52TVNDD168 pKa = 4.88LIYY171 pKa = 11.12NKK173 pKa = 10.51AVFQFSSGHH182 pKa = 5.1QFQQVEE188 pKa = 4.25PGLMKK193 pKa = 10.61SGLVRR198 pKa = 11.84TLSTNSHH205 pKa = 5.59CQCFIHH211 pKa = 6.86WLAEE215 pKa = 4.19QKK217 pKa = 10.35CGHH220 pKa = 5.51SHH222 pKa = 6.45KK223 pKa = 10.26FWTIGDD229 pKa = 4.29DD230 pKa = 4.24LLVDD234 pKa = 4.88DD235 pKa = 5.52PCDD238 pKa = 3.84DD239 pKa = 3.84YY240 pKa = 11.97LRR242 pKa = 11.84YY243 pKa = 8.5TSDD246 pKa = 3.31YY247 pKa = 10.5CILKK251 pKa = 10.1EE252 pKa = 4.41VEE254 pKa = 3.84PGYY257 pKa = 11.27NFAGFNLKK265 pKa = 8.96TKK267 pKa = 9.96TPLYY271 pKa = 8.22WSKK274 pKa = 10.79HH275 pKa = 4.17IYY277 pKa = 9.45RR278 pKa = 11.84LLYY281 pKa = 10.06VDD283 pKa = 4.48EE284 pKa = 4.73KK285 pKa = 11.21VIPDD289 pKa = 3.59VLDD292 pKa = 3.62NYY294 pKa = 10.18QRR296 pKa = 11.84LYY298 pKa = 10.97VYY300 pKa = 10.61SDD302 pKa = 2.7KK303 pKa = 11.07KK304 pKa = 11.18FNIFQDD310 pKa = 3.44MLYY313 pKa = 10.79DD314 pKa = 4.68LDD316 pKa = 3.79PTKK319 pKa = 10.45IRR321 pKa = 11.84SQAYY325 pKa = 7.79LKK327 pKa = 10.23RR328 pKa = 11.84WAVQKK333 pKa = 10.44PSNFKK338 pKa = 10.66FLINN342 pKa = 3.65

Molecular weight: 40.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEL5|A0A1L3KEL5_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 38 OX=1923225 PE=4 SV=1
MM1 pKa = 7.35TPLLPHH7 pKa = 6.75LQMNHH12 pKa = 4.47DD13 pKa = 4.73TIRR16 pKa = 11.84LEE18 pKa = 4.14SNLRR22 pKa = 11.84VYY24 pKa = 9.6GTVPNPSMQDD34 pKa = 3.29GPLHH38 pKa = 6.35NLYY41 pKa = 10.7CGLKK45 pKa = 10.14QKK47 pKa = 8.79TEE49 pKa = 4.13WNVMLALGGLEE60 pKa = 4.32TFWLLRR66 pKa = 11.84LIVYY70 pKa = 7.95LVKK73 pKa = 10.26MVGCVCLRR81 pKa = 11.84LLRR84 pKa = 11.84LFKK87 pKa = 10.55FVILRR92 pKa = 11.84LSIIMMNWQLWKK104 pKa = 11.1YY105 pKa = 10.9LFLCLLLYY113 pKa = 10.51KK114 pKa = 10.78LNLLKK119 pKa = 10.4FVPQMMQWFASQVVEE134 pKa = 6.01LYY136 pKa = 10.66DD137 pKa = 3.35QLPPVSSLLRR147 pKa = 11.84TFLEE151 pKa = 5.16CINILEE157 pKa = 4.31PQKK160 pKa = 11.09KK161 pKa = 9.94DD162 pKa = 3.17IPVLHH167 pKa = 6.89IWLEE171 pKa = 4.35TIQWPLCICSVVMLVICVCQPSTFKK196 pKa = 10.48WLSNNLLCQQSLGTSLGFMARR217 pKa = 11.84FWLRR221 pKa = 11.84QMTSLLAQASLNLSYY236 pKa = 10.03VTLVIVIPKK245 pKa = 10.01LIFVIKK251 pKa = 10.3DD252 pKa = 3.25YY253 pKa = 11.35LLNLVKK259 pKa = 10.64RR260 pKa = 11.84NANSRR265 pKa = 11.84SLMTGMKK272 pKa = 10.4KK273 pKa = 9.0MFSLAKK279 pKa = 10.46LFVLVVLGLTRR290 pKa = 11.84TVTNFNLAVIIILLTMMVFSGFGGLLISVVVRR322 pKa = 11.84LSWALLWALPLMVNPYY338 pKa = 9.56IIMMMFVLMKK348 pKa = 9.91MRR350 pKa = 11.84ILFF353 pKa = 3.98

Molecular weight: 40.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1181

342

486

393.7

45.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.234 ± 0.626

2.371 ± 0.32

5.419 ± 1.628

3.98 ± 0.815

5.165 ± 0.594

5.25 ± 0.788

1.778 ± 0.537

5.165 ± 0.571

6.097 ± 0.727

12.955 ± 2.976

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.556 ± 1.173

4.149 ± 0.567

4.742 ± 0.189

4.488 ± 0.182

4.657 ± 0.688

7.113 ± 0.946

5.165 ± 0.752

7.197 ± 1.014

2.625 ± 0.44

3.895 ± 0.629
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski