Beihai barnacle virus 11
Average proteome isoelectric point is 7.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFS1|A0A1L3KFS1_9VIRU Capsid protein OS=Beihai barnacle virus 11 OX=1922355 PE=4 SV=1
MM1 pKa = 7.55 EE2 pKa = 5.39 SNVAITVQPNDD13 pKa = 3.08 IGSFFSLRR21 pKa = 11.84 SLPRR25 pKa = 11.84 VVAKK29 pKa = 10.05 TGGICLLGVAGYY41 pKa = 10.34 KK42 pKa = 9.86 IVTSSWLAACVEE54 pKa = 4.4 DD55 pKa = 4.71 LRR57 pKa = 11.84 HH58 pKa = 6.7 RR59 pKa = 11.84 LISKK63 pKa = 9.83 NKK65 pKa = 8.95 TIIPEE70 pKa = 4.16 AGRR73 pKa = 11.84 VRR75 pKa = 11.84 AFVQKK80 pKa = 10.44 EE81 pKa = 3.97 MEE83 pKa = 4.24 LVSTSVRR90 pKa = 11.84 KK91 pKa = 9.31 GHH93 pKa = 5.46 SHH95 pKa = 5.14 STAASEE101 pKa = 4.25 RR102 pKa = 11.84 NSATEE107 pKa = 3.67 TMIDD111 pKa = 3.58 VITTNGYY118 pKa = 8.32 EE119 pKa = 4.27 PYY121 pKa = 10.27 VISPSPRR128 pKa = 11.84 EE129 pKa = 3.72 SDD131 pKa = 3.04 LDD133 pKa = 3.66 GVRR136 pKa = 11.84 RR137 pKa = 11.84 FHH139 pKa = 7.27 SLADD143 pKa = 3.35 LRR145 pKa = 11.84 QDD147 pKa = 3.44 YY148 pKa = 10.22 RR149 pKa = 11.84 RR150 pKa = 11.84 SPITDD155 pKa = 2.82 RR156 pKa = 11.84 HH157 pKa = 6.46 IIVMTDD163 pKa = 2.0 VDD165 pKa = 4.87 YY166 pKa = 11.4 YY167 pKa = 11.67 VDD169 pKa = 3.25 MHH171 pKa = 6.66 EE172 pKa = 5.03 IISIGRR178 pKa = 11.84 PILLYY183 pKa = 9.16 TFQPRR188 pKa = 11.84 VVSGPVFDD196 pKa = 5.28 GFFTIKK202 pKa = 10.75 DD203 pKa = 3.76 NIIHH207 pKa = 5.63 YY208 pKa = 9.56 RR209 pKa = 11.84 VNGGKK214 pKa = 9.43 DD215 pKa = 3.32 VKK217 pKa = 10.98 HH218 pKa = 5.69 PTWNYY223 pKa = 8.83 NQDD226 pKa = 3.34 TVFVRR231 pKa = 11.84 DD232 pKa = 3.59 PVNTFWEE239 pKa = 4.47 TVYY242 pKa = 11.21 SVFRR246 pKa = 11.84 EE247 pKa = 3.98 VTGISWIDD255 pKa = 3.12 RR256 pKa = 11.84 MIYY259 pKa = 9.83 HH260 pKa = 7.06 YY261 pKa = 10.58 IGIAPSGRR269 pKa = 11.84 RR270 pKa = 11.84 LTMCTIDD277 pKa = 3.58 QFEE280 pKa = 4.67 LSPHH284 pKa = 6.06 RR285 pKa = 11.84 NIVSIVPFAHH295 pKa = 6.49 CRR297 pKa = 11.84 EE298 pKa = 4.16 NLLPAAEE305 pKa = 4.44 YY306 pKa = 8.82 GTEE309 pKa = 4.17 LSRR312 pKa = 11.84 SNYY315 pKa = 8.11 QQAAGCPRR323 pKa = 11.84 MNAITYY329 pKa = 10.12 IGDD332 pKa = 3.76 GDD334 pKa = 4.12 PLISLGEE341 pKa = 4.06 EE342 pKa = 4.2 GNFASVQLPLKK353 pKa = 10.26 DD354 pKa = 4.1 LEE356 pKa = 4.51 SLRR359 pKa = 11.84 TAYY362 pKa = 10.36 HH363 pKa = 6.72 LSKK366 pKa = 10.02 TNNLSDD372 pKa = 3.38 TVRR375 pKa = 11.84 RR376 pKa = 11.84 SKK378 pKa = 11.2 RR379 pKa = 11.84 NDD381 pKa = 2.82 KK382 pKa = 9.92 EE383 pKa = 3.62 AAIIHH388 pKa = 5.47 QFLVSEE394 pKa = 4.74 SEE396 pKa = 3.95 LHH398 pKa = 5.73 PVEE401 pKa = 4.09 VHH403 pKa = 6.96 KK404 pKa = 10.66 PGQLARR410 pKa = 11.84 HH411 pKa = 4.95 YY412 pKa = 10.69 QSVEE416 pKa = 3.62 KK417 pKa = 10.93 DD418 pKa = 3.06 HH419 pKa = 7.34 DD420 pKa = 4.13 VDD422 pKa = 3.41 PTEE425 pKa = 3.84 QGKK428 pKa = 10.12 EE429 pKa = 3.95 YY430 pKa = 10.76 ARR432 pKa = 11.84 EE433 pKa = 3.96 FAPGPLTQTAVFPSEE448 pKa = 4.09 STSNEE453 pKa = 3.39 RR454 pKa = 11.84 ATIEE458 pKa = 3.74 GRR460 pKa = 11.84 INVPQAKK467 pKa = 9.75 AKK469 pKa = 9.83 AKK471 pKa = 10.23 EE472 pKa = 4.25 RR473 pKa = 11.84 ITPRR477 pKa = 11.84 LRR479 pKa = 11.84 RR480 pKa = 11.84 IARR483 pKa = 11.84 DD484 pKa = 3.65 FVKK487 pKa = 10.66 HH488 pKa = 5.82 LVPEE492 pKa = 4.62 VGAGHH497 pKa = 7.71 PYY499 pKa = 10.09 SGSHH503 pKa = 6.16 VEE505 pKa = 4.22 EE506 pKa = 4.35 QQQKK510 pKa = 8.97 PLQRR514 pKa = 11.84 ARR516 pKa = 11.84 NDD518 pKa = 3.01 ANRR521 pKa = 11.84 FHH523 pKa = 7.62 DD524 pKa = 4.5 AFDD527 pKa = 3.61 MVTKK531 pKa = 10.68 AFQKK535 pKa = 10.4 RR536 pKa = 11.84 EE537 pKa = 3.91 AYY539 pKa = 8.61 NAPNHH544 pKa = 6.13 PRR546 pKa = 11.84 NISTVPHH553 pKa = 5.58 GQNVKK558 pKa = 10.69 LSGFTYY564 pKa = 10.44 AFKK567 pKa = 10.89 DD568 pKa = 4.5 AILKK572 pKa = 7.43 NQKK575 pKa = 9.04 WYY577 pKa = 9.27 MPCHH581 pKa = 6.07 TPSEE585 pKa = 4.43 IATMVQDD592 pKa = 4.33 LAAEE596 pKa = 4.2 STEE599 pKa = 4.32 LVEE602 pKa = 4.1 TDD604 pKa = 3.26 YY605 pKa = 11.77 SRR607 pKa = 11.84 FDD609 pKa = 3.3 GTFLRR614 pKa = 11.84 FMRR617 pKa = 11.84 EE618 pKa = 3.98 SVEE621 pKa = 3.71 FAAYY625 pKa = 9.38 KK626 pKa = 10.08 RR627 pKa = 11.84 WTHH630 pKa = 6.09 SDD632 pKa = 2.93 HH633 pKa = 6.98 SQEE636 pKa = 5.51 LDD638 pKa = 3.37 DD639 pKa = 5.84 LLANEE644 pKa = 4.84 IGSKK648 pKa = 10.41 AVTRR652 pKa = 11.84 KK653 pKa = 9.38 GLKK656 pKa = 9.57 YY657 pKa = 10.77 LPDD660 pKa = 3.96 CSRR663 pKa = 11.84 LSGSPLTTDD672 pKa = 3.56 GNSIANAFVSYY683 pKa = 10.38 AANRR687 pKa = 11.84 LSGMKK692 pKa = 9.66 TEE694 pKa = 4.29 EE695 pKa = 3.49 AWLNIGIVYY704 pKa = 10.5 GDD706 pKa = 3.68 DD707 pKa = 3.43 GLRR710 pKa = 11.84 NGTVSDD716 pKa = 5.09 AILMEE721 pKa = 4.49 TASSLGFDD729 pKa = 3.74 LKK731 pKa = 10.73 IINRR735 pKa = 11.84 ASRR738 pKa = 11.84 GQPVSFLSRR747 pKa = 11.84 IYY749 pKa = 10.77 ADD751 pKa = 3.62 PWSSPASVQSPTRR764 pKa = 11.84 TLLKK768 pKa = 10.35 IHH770 pKa = 6.0 TTCDD774 pKa = 2.82 AHH776 pKa = 9.05 SDD778 pKa = 3.45 IEE780 pKa = 4.35 EE781 pKa = 4.21 VGWAKK786 pKa = 8.65 TQAYY790 pKa = 9.71 LVTDD794 pKa = 4.32 GLTPFISHH802 pKa = 6.13 WCKK805 pKa = 10.35 AYY807 pKa = 10.46 QRR809 pKa = 11.84 NCTSKK814 pKa = 10.34 IVDD817 pKa = 3.63 YY818 pKa = 11.37 KK819 pKa = 11.32 DD820 pKa = 3.3 FTDD823 pKa = 3.19 IPFWVRR829 pKa = 11.84 DD830 pKa = 3.76 EE831 pKa = 4.79 NSLNNSWPQNDD842 pKa = 3.16 SDD844 pKa = 3.73 EE845 pKa = 4.35 WNKK848 pKa = 9.88 IVAEE852 pKa = 4.04 DD853 pKa = 3.82 LGVSVSEE860 pKa = 4.91 LMEE863 pKa = 4.42 HH864 pKa = 6.87 LKK866 pKa = 10.99 KK867 pKa = 10.6 LDD869 pKa = 4.03 GYY871 pKa = 10.48 NGPISEE877 pKa = 4.79 LPRR880 pKa = 11.84 LATNIKK886 pKa = 10.08 LDD888 pKa = 3.73 PKK890 pKa = 10.64 LAVALDD896 pKa = 4.28 GEE898 pKa = 4.91 VHH900 pKa = 7.26 AGPTKK905 pKa = 10.69 QEE907 pKa = 4.45 DD908 pKa = 4.31 GQDD911 pKa = 3.73 NPSDD915 pKa = 3.76 QPASGTAPTAVPTTGGRR932 pKa = 11.84 PQKK935 pKa = 10.11 PGRR938 pKa = 11.84 ADD940 pKa = 3.41 RR941 pKa = 11.84 AGRR944 pKa = 11.84 QRR946 pKa = 11.84 NAHH949 pKa = 6.37 LRR951 pKa = 11.84 DD952 pKa = 3.6 QRR954 pKa = 11.84 PSRR957 pKa = 11.84 SDD959 pKa = 2.65 KK960 pKa = 9.63 TARR963 pKa = 11.84 PDD965 pKa = 3.5 RR966 pKa = 11.84 QVSGPSDD973 pKa = 3.67 NGPGNPSSGRR983 pKa = 11.84 KK984 pKa = 7.66 STPQVGGKK992 pKa = 7.37 KK993 pKa = 7.46 TRR995 pKa = 11.84 KK996 pKa = 9.32 AA997 pKa = 3.32
Molecular weight: 111.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.759
IPC2_protein 6.985
IPC_protein 6.985
Toseland 7.059
ProMoST 7.497
Dawson 7.571
Bjellqvist 7.673
Wikipedia 7.527
Rodwell 7.571
Grimsley 7.205
Solomon 7.6
Lehninger 7.614
Nozaki 7.907
DTASelect 7.805
Thurlkill 7.849
EMBOSS 7.878
Sillero 7.995
Patrickios 4.495
IPC_peptide 7.6
IPC2_peptide 7.22
IPC2.peptide.svr19 7.068
Protein with the highest isoelectric point:
>tr|A0A1L3KFS1|A0A1L3KFS1_9VIRU Capsid protein OS=Beihai barnacle virus 11 OX=1922355 PE=4 SV=1
MM1 pKa = 7.88 ANGRR5 pKa = 11.84 KK6 pKa = 9.48 RR7 pKa = 11.84 NNNNNRR13 pKa = 11.84 RR14 pKa = 11.84 PAQNPQAAPNPRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 PRR30 pKa = 11.84 MRR32 pKa = 11.84 RR33 pKa = 11.84 GTGGVALVGSNQSTLRR49 pKa = 11.84 VNNPTGGRR57 pKa = 11.84 AQGGSDD63 pKa = 4.91 LIAALTIPSSVEE75 pKa = 3.56 QGEE78 pKa = 4.35 LLVDD82 pKa = 4.29 VIVTPSVAARR92 pKa = 11.84 LEE94 pKa = 4.19 TLATAWQRR102 pKa = 11.84 LKK104 pKa = 10.39 YY105 pKa = 8.92 HH106 pKa = 6.76 KK107 pKa = 11.06 LMFEE111 pKa = 4.44 VNASGSSLIGGEE123 pKa = 4.16 FVAAFIADD131 pKa = 3.89 PTDD134 pKa = 3.32 VPPLRR139 pKa = 11.84 GADD142 pKa = 3.0 RR143 pKa = 11.84 WVKK146 pKa = 9.64 AHH148 pKa = 6.09 QGSVTSSWWRR158 pKa = 11.84 SVNVHH163 pKa = 6.29 GPCPPQVMYY172 pKa = 10.3 TSYY175 pKa = 10.99 EE176 pKa = 3.89 PNEE179 pKa = 4.09 PRR181 pKa = 11.84 FSSPGRR187 pKa = 11.84 FVLAVVNPPSSDD199 pKa = 2.88 TTMSISLNWHH209 pKa = 4.27 VTFMQPSLEE218 pKa = 4.08 YY219 pKa = 10.14 TIEE222 pKa = 3.92 EE223 pKa = 3.92 DD224 pKa = 3.31 SIYY227 pKa = 11.02 VLEE230 pKa = 4.23 EE231 pKa = 3.64 DD232 pKa = 5.16 ARR234 pKa = 11.84 LKK236 pKa = 10.41 LTDD239 pKa = 4.67 FSAGQPFDD247 pKa = 3.2 PHH249 pKa = 6.75 LRR251 pKa = 11.84 KK252 pKa = 10.2 GLDD255 pKa = 3.51 GKK257 pKa = 10.69 DD258 pKa = 3.02 GTAYY262 pKa = 10.88 LSPEE266 pKa = 4.63 DD267 pKa = 3.98 FTPTLPEE274 pKa = 3.82 GVMLRR279 pKa = 11.84 LPHH282 pKa = 6.88 PKK284 pKa = 8.91 TLNADD289 pKa = 3.79 TGASGAPEE297 pKa = 4.0 NAIVTHH303 pKa = 6.13 MGVSIAAGSRR313 pKa = 11.84 RR314 pKa = 11.84 IAYY317 pKa = 8.51 FYY319 pKa = 10.63 STDD322 pKa = 3.68 DD323 pKa = 3.2 VHH325 pKa = 5.93 YY326 pKa = 8.25 TQLDD330 pKa = 3.24 ASLPFSIRR338 pKa = 11.84 PVADD342 pKa = 4.68 PINKK346 pKa = 9.68 KK347 pKa = 8.51 GTVYY351 pKa = 10.78 EE352 pKa = 4.1 EE353 pKa = 4.67 DD354 pKa = 3.55 VLEE357 pKa = 4.59 SGNATGALARR367 pKa = 11.84 SARR370 pKa = 11.84 VPRR373 pKa = 11.84 SLTSAPALRR382 pKa = 11.84 QSSKK386 pKa = 11.18 GKK388 pKa = 10.05 LLSFQNSKK396 pKa = 10.71 RR397 pKa = 11.84 FF398 pKa = 3.37
Molecular weight: 43.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.799
IPC2_protein 8.756
IPC_protein 8.799
Toseland 9.297
ProMoST 9.151
Dawson 9.619
Bjellqvist 9.355
Wikipedia 9.823
Rodwell 9.765
Grimsley 9.706
Solomon 9.677
Lehninger 9.633
Nozaki 9.341
DTASelect 9.341
Thurlkill 9.428
EMBOSS 9.736
Sillero 9.545
Patrickios 5.016
IPC_peptide 9.663
IPC2_peptide 7.966
IPC2.peptide.svr19 7.769
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1395
398
997
697.5
77.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.957 ± 0.656
0.789 ± 0.263
6.022 ± 0.611
5.305 ± 0.383
3.297 ± 0.015
6.667 ± 0.427
2.867 ± 0.42
4.946 ± 0.823
4.516 ± 0.612
7.168 ± 0.55
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.649 ± 0.177
4.803 ± 0.232
6.667 ± 0.796
3.584 ± 0.156
7.312 ± 0.111
8.746 ± 0.393
6.523 ± 0.005
6.882 ± 0.198
1.29 ± 0.017
3.011 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here