Pseudo-nitzschia multistriata

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta; Bacillariophyceae; Bacillariophycidae; Bacillariales; Bacillariaceae; Pseudo-nitzschia

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11907 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A448ZGN2|A0A448ZGN2_9STRA Helicase ATP-binding domain-containing protein OS=Pseudo-nitzschia multistriata OX=183589 GN=PSNMU_V1.4_AUG-EV-PASAV3_0081690 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 7.78PTRR5 pKa = 11.84SSRR8 pKa = 11.84FLLLVLLFLVSFLVRR23 pKa = 11.84STMGEE28 pKa = 4.03TLLVKK33 pKa = 10.61AQDD36 pKa = 3.96EE37 pKa = 4.67DD38 pKa = 3.88EE39 pKa = 4.98DD40 pKa = 4.61AADD43 pKa = 4.1TPAVADD49 pKa = 4.22DD50 pKa = 5.01GGDD53 pKa = 3.42EE54 pKa = 4.5TEE56 pKa = 4.87TDD58 pKa = 3.71TEE60 pKa = 4.12TDD62 pKa = 3.34PAAEE66 pKa = 4.53GDD68 pKa = 3.9AEE70 pKa = 4.52GDD72 pKa = 3.6GDD74 pKa = 5.35DD75 pKa = 4.59GDD77 pKa = 5.06GEE79 pKa = 4.28EE80 pKa = 5.47GDD82 pKa = 5.26DD83 pKa = 4.28GDD85 pKa = 4.62EE86 pKa = 4.48EE87 pKa = 4.96SDD89 pKa = 3.49EE90 pKa = 4.3TGEE93 pKa = 4.46EE94 pKa = 4.2GDD96 pKa = 5.68AEE98 pKa = 5.0DD99 pKa = 5.34DD100 pKa = 3.98DD101 pKa = 5.89EE102 pKa = 5.15GFQVEE107 pKa = 4.37DD108 pKa = 4.17LLPGFEE114 pKa = 4.71NTDD117 pKa = 2.57ADD119 pKa = 4.12NFVKK123 pKa = 10.56GYY125 pKa = 10.38ILGNPEE131 pKa = 4.37YY132 pKa = 10.28YY133 pKa = 9.83QEE135 pKa = 5.47LYY137 pKa = 10.36VAPKK141 pKa = 10.62AVTKK145 pKa = 10.22FNNLPRR151 pKa = 11.84FGHH154 pKa = 6.18SDD156 pKa = 3.69DD157 pKa = 4.8FSLLMGDD164 pKa = 4.43YY165 pKa = 9.83PDD167 pKa = 4.63HH168 pKa = 7.05YY169 pKa = 11.24YY170 pKa = 11.07LGLLFVGTLILTIFFFWGIAIGIFKK195 pKa = 10.61CIGPKK200 pKa = 10.06HH201 pKa = 6.09VGFLAGFPFQVEE213 pKa = 4.68GKK215 pKa = 9.31KK216 pKa = 10.07SAAGRR221 pKa = 11.84VVFSVGAWMMIVSTIFLISLGLPRR245 pKa = 11.84MQYY248 pKa = 10.91LSDD251 pKa = 4.45SIAFTADD258 pKa = 3.5EE259 pKa = 3.96IQIVEE264 pKa = 4.44KK265 pKa = 9.45EE266 pKa = 3.97VKK268 pKa = 10.75YY269 pKa = 10.7IVTTLLVVSAKK280 pKa = 9.08TKK282 pKa = 9.75PIRR285 pKa = 11.84DD286 pKa = 3.76EE287 pKa = 4.54LVDD290 pKa = 3.8FLKK293 pKa = 10.62RR294 pKa = 11.84DD295 pKa = 3.49ICPLEE300 pKa = 4.57PGSDD304 pKa = 3.3TEE306 pKa = 4.22DD307 pKa = 3.18QVRR310 pKa = 11.84SVGEE314 pKa = 3.9DD315 pKa = 3.07TYY317 pKa = 11.93DD318 pKa = 3.94AMTDD322 pKa = 3.33LEE324 pKa = 5.06DD325 pKa = 5.01FIEE328 pKa = 5.88GYY330 pKa = 10.82LLDD333 pKa = 4.6VEE335 pKa = 5.82DD336 pKa = 5.74GVNTTNTFTSNILAVTDD353 pKa = 3.41MAQFVGNPVVTAIVFPYY370 pKa = 9.57FIIPIILLVAVGMGWYY386 pKa = 9.91DD387 pKa = 3.97FYY389 pKa = 11.89SEE391 pKa = 4.22TFYY394 pKa = 11.68SFVTWLVVPLLVLLTILAFVTATTLVVAIQANADD428 pKa = 3.36ICVSSGAPEE437 pKa = 4.16DD438 pKa = 4.35SILGVLNNLDD448 pKa = 4.56LEE450 pKa = 4.9DD451 pKa = 5.57DD452 pKa = 4.26SGSQSKK458 pKa = 10.81DD459 pKa = 3.28DD460 pKa = 4.91NGPSDD465 pKa = 3.52NFFYY469 pKa = 10.93DD470 pKa = 2.72IMVFYY475 pKa = 8.94THH477 pKa = 6.47QCTKK481 pKa = 9.45PNPWEE486 pKa = 4.0FMEE489 pKa = 4.5GHH491 pKa = 6.0YY492 pKa = 11.08ADD494 pKa = 4.74LARR497 pKa = 11.84GRR499 pKa = 11.84NILGEE504 pKa = 4.43FIRR507 pKa = 11.84SIEE510 pKa = 4.06DD511 pKa = 3.3TTLLEE516 pKa = 4.43LSQEE520 pKa = 4.48CGMEE524 pKa = 3.8YY525 pKa = 11.05GPIVVLLKK533 pKa = 10.68SLQDD537 pKa = 4.59LITILSQTSIRR548 pKa = 11.84ALTLMSCRR556 pKa = 11.84NVVPLYY562 pKa = 8.76TAWIYY567 pKa = 11.17EE568 pKa = 4.57STCTLAPVSLNLVFMCIVCIAFFGMVCITFRR599 pKa = 11.84GAYY602 pKa = 9.35YY603 pKa = 10.03PIDD606 pKa = 4.19FYY608 pKa = 11.76YY609 pKa = 11.06YY610 pKa = 10.35DD611 pKa = 3.3ATGRR615 pKa = 11.84GDD617 pKa = 3.52KK618 pKa = 10.69QEE620 pKa = 5.08LYY622 pKa = 8.74PTDD625 pKa = 3.58EE626 pKa = 4.52VGDD629 pKa = 3.97SGLADD634 pKa = 3.74TPCNEE639 pKa = 4.27KK640 pKa = 10.69VLEE643 pKa = 4.16EE644 pKa = 4.99QEE646 pKa = 4.36DD647 pKa = 3.75QCIDD651 pKa = 3.01ATEE654 pKa = 3.96AMEE657 pKa = 4.2EE658 pKa = 4.19SIEE661 pKa = 3.87IQIYY665 pKa = 10.33HH666 pKa = 7.27DD667 pKa = 4.51DD668 pKa = 3.8EE669 pKa = 4.5EE670 pKa = 5.75KK671 pKa = 10.66EE672 pKa = 4.15YY673 pKa = 9.86EE674 pKa = 4.18TDD676 pKa = 5.1DD677 pKa = 5.65DD678 pKa = 4.36EE679 pKa = 5.51QDD681 pKa = 3.3HH682 pKa = 6.55EE683 pKa = 5.72KK684 pKa = 11.21NFLLDD689 pKa = 3.65KK690 pKa = 10.67YY691 pKa = 9.36GEE693 pKa = 4.19PMEE696 pKa = 5.25GPDD699 pKa = 3.83AAQHH703 pKa = 5.65SVGTNGSYY711 pKa = 10.33RR712 pKa = 11.84GIDD715 pKa = 3.31RR716 pKa = 11.84NGDD719 pKa = 3.17SHH721 pKa = 7.78AVYY724 pKa = 10.74SDD726 pKa = 4.85DD727 pKa = 4.02DD728 pKa = 4.52DD729 pKa = 5.17EE730 pKa = 4.74MTFHH734 pKa = 6.58STFDD738 pKa = 3.91DD739 pKa = 4.17EE740 pKa = 5.16YY741 pKa = 9.5EE742 pKa = 4.24YY743 pKa = 11.69NSAAEE748 pKa = 4.32EE749 pKa = 3.97EE750 pKa = 4.95SEE752 pKa = 4.33TFSYY756 pKa = 11.28VSS758 pKa = 3.3

Molecular weight: 84.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A448ZSB5|A0A448ZSB5_9STRA Uncharacterized protein OS=Pseudo-nitzschia multistriata OX=183589 GN=PSNMU_V1.4_AUG-EV-PASAV3_0120360 PE=4 SV=1
MM1 pKa = 7.24VGAIPPGASGSLQNPLLPPHH21 pKa = 6.89RR22 pKa = 11.84SPWTMLATTGSPPRR36 pKa = 11.84ASGEE40 pKa = 3.95RR41 pKa = 11.84SSLVFDD47 pKa = 4.24RR48 pKa = 11.84RR49 pKa = 11.84ASILSEE55 pKa = 3.72GRR57 pKa = 11.84PPAFPVSLSMWPSRR71 pKa = 11.84CSSQKK76 pKa = 9.38SAQFSRR82 pKa = 11.84QGRR85 pKa = 11.84FGWKK89 pKa = 7.86VAPMVLSTGNPKK101 pKa = 10.18GASPFGDD108 pKa = 3.65TPALCIFASATPSSYY123 pKa = 10.64CPPGPLPQNSRR134 pKa = 11.84YY135 pKa = 9.89SKK137 pKa = 10.34RR138 pKa = 11.84AKK140 pKa = 9.71AWSLASCLPKK150 pKa = 10.61AIGLGTRR157 pKa = 11.84MVEE160 pKa = 4.14VSDD163 pKa = 3.71MALTPSYY170 pKa = 10.31SARR173 pKa = 11.84SMSYY177 pKa = 10.16PFRR180 pKa = 11.84ADD182 pKa = 2.91LRR184 pKa = 11.84KK185 pKa = 9.95YY186 pKa = 9.56RR187 pKa = 11.84VSVSASRR194 pKa = 11.84RR195 pKa = 11.84APVLAPPPPEE205 pKa = 4.36APPPVVGVSFTSLPRR220 pKa = 11.84VSDD223 pKa = 3.58AAVDD227 pKa = 3.85TVSRR231 pKa = 11.84KK232 pKa = 8.48VAAARR237 pKa = 11.84MAVEE241 pKa = 4.31GQPLVLLLMLLMLLLLLMLLPAAKK265 pKa = 10.14DD266 pKa = 3.31RR267 pKa = 11.84AGWAKK272 pKa = 10.57NPPPEE277 pKa = 4.24NADD280 pKa = 2.72ARR282 pKa = 11.84AAMEE286 pKa = 4.19APTRR290 pKa = 11.84SVLPDD295 pKa = 3.33RR296 pKa = 11.84LEE298 pKa = 4.07LKK300 pKa = 10.24PRR302 pKa = 11.84GRR304 pKa = 11.84TRR306 pKa = 11.84IMVCVRR312 pKa = 11.84FGLVLLAEE320 pKa = 4.72ASLEE324 pKa = 3.76RR325 pKa = 11.84SMYY328 pKa = 10.52SILRR332 pKa = 11.84CHH334 pKa = 6.62PNVPLRR340 pKa = 11.84CEE342 pKa = 3.77EE343 pKa = 4.07VLVVVTVPDD352 pKa = 3.59VTYY355 pKa = 10.63DD356 pKa = 2.77QYY358 pKa = 11.94DD359 pKa = 3.15RR360 pKa = 11.84HH361 pKa = 5.88RR362 pKa = 11.84RR363 pKa = 11.84NRR365 pKa = 11.84AKK367 pKa = 10.53SVFCCLEE374 pKa = 3.61QNPWIFSFKK383 pKa = 9.72MKK385 pKa = 9.61RR386 pKa = 11.84TRR388 pKa = 3.32

Molecular weight: 42.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11763

144

11907

6325991

13

5812

531.3

58.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.042 ± 0.026

1.526 ± 0.009

6.31 ± 0.019

6.919 ± 0.025

3.46 ± 0.014

7.121 ± 0.026

2.241 ± 0.011

4.364 ± 0.014

5.531 ± 0.028

8.318 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.166 ± 0.009

4.428 ± 0.014

5.211 ± 0.021

3.68 ± 0.017

6.215 ± 0.022

8.918 ± 0.025

5.769 ± 0.014

5.95 ± 0.018

1.115 ± 0.007

2.393 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski