Shearwaterpox virus
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 305 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V0S7S5|A0A1V0S7S5_CNPV DNA-directed RNA polymerase 18 kDa subunit OS=Shearwaterpox virus OX=1974596 GN=SWPV1-068 PE=3 SV=1
MM1 pKa = 7.18 IVKK4 pKa = 8.37 MHH6 pKa = 7.47 LINIFVCIICINLTYY21 pKa = 10.18 SYY23 pKa = 10.74 RR24 pKa = 11.84 IIDD27 pKa = 4.08 FSNGANNKK35 pKa = 9.26 IFGSGSGSGSEE46 pKa = 4.2 NGSGSGSGDD55 pKa = 3.46 DD56 pKa = 4.75 ALPSSITNIISRR68 pKa = 11.84 DD69 pKa = 3.15 GSGYY73 pKa = 10.63 DD74 pKa = 3.85 DD75 pKa = 4.86 GSVTTDD81 pKa = 2.62 ISVTTDD87 pKa = 2.59 ISVTTDD93 pKa = 2.74 SSVTTDD99 pKa = 3.0 SSVTTDD105 pKa = 3.0 SSVTTDD111 pKa = 3.04 SSVTIDD117 pKa = 3.95 NDD119 pKa = 3.38 NKK121 pKa = 10.46 YY122 pKa = 11.03 LSILTIIYY130 pKa = 10.74 SMLRR134 pKa = 11.84 SFFSAA139 pKa = 3.79
Molecular weight: 14.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.859
IPC_protein 3.859
Toseland 3.617
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.05
Wikipedia 3.897
Rodwell 3.681
Grimsley 3.528
Solomon 3.872
Lehninger 3.834
Nozaki 4.012
DTASelect 4.342
Thurlkill 3.706
EMBOSS 3.897
Sillero 3.986
Patrickios 1.952
IPC_peptide 3.859
IPC2_peptide 3.948
IPC2.peptide.svr19 3.88
Protein with the highest isoelectric point:
>tr|A0A1V0S809|A0A1V0S809_CNPV SWPV1-113 OS=Shearwaterpox virus OX=1974596 GN=SWPV1-113 PE=4 SV=1
MM1 pKa = 7.28 IRR3 pKa = 11.84 IIFKK7 pKa = 10.41 YY8 pKa = 9.69 NSIYY12 pKa = 8.53 VTRR15 pKa = 11.84 LLVLTLILLISVFLLRR31 pKa = 11.84 RR32 pKa = 11.84 ISFIYY37 pKa = 10.46 KK38 pKa = 10.4 NILLLVKK45 pKa = 9.46 MDD47 pKa = 3.83 NMCNTIMM54 pKa = 4.96
Molecular weight: 6.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.298
IPC2_protein 9.955
IPC_protein 10.526
Toseland 10.379
ProMoST 10.116
Dawson 10.57
Bjellqvist 10.292
Wikipedia 10.774
Rodwell 10.891
Grimsley 10.657
Solomon 10.628
Lehninger 10.599
Nozaki 10.379
DTASelect 10.277
Thurlkill 10.423
EMBOSS 10.774
Sillero 10.482
Patrickios 10.716
IPC_peptide 10.628
IPC2_peptide 9.326
IPC2.peptide.svr19 8.333
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
305
0
305
97274
47
1939
318.9
36.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.356 ± 0.102
2.266 ± 0.081
6.317 ± 0.086
5.67 ± 0.109
4.136 ± 0.105
3.718 ± 0.096
1.94 ± 0.047
10.441 ± 0.175
8.698 ± 0.134
9.236 ± 0.143
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.432 ± 0.047
7.899 ± 0.146
2.813 ± 0.083
1.83 ± 0.067
3.871 ± 0.097
7.684 ± 0.151
5.399 ± 0.096
5.674 ± 0.086
0.655 ± 0.034
5.966 ± 0.079
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here