Flavobacterium sp. 9AF

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3815 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653VZH5|A0A653VZH5_9FLAO Aspartate aminotransferase family protein OS=Flavobacterium sp. 9AF OX=2653142 GN=FLAVO9AF_60008 PE=3 SV=1
MM1 pKa = 7.74ANDD4 pKa = 4.72LPQDD8 pKa = 4.12VIPLEE13 pKa = 4.53TAQQWAANWNQEE25 pKa = 3.84GANYY29 pKa = 9.78LANNALKK36 pKa = 10.82AFLIPGADD44 pKa = 2.9IQGIIKK50 pKa = 10.24DD51 pKa = 4.01DD52 pKa = 3.34NTYY55 pKa = 10.73DD56 pKa = 3.05IRR58 pKa = 11.84GYY60 pKa = 10.94LGLEE64 pKa = 4.08KK65 pKa = 10.71LSDD68 pKa = 3.85GSFEE72 pKa = 3.89PHH74 pKa = 5.74MMVVGVDD81 pKa = 3.29NNQNDD86 pKa = 3.97MIDD89 pKa = 3.66YY90 pKa = 10.6DD91 pKa = 3.8KK92 pKa = 11.33GYY94 pKa = 10.63YY95 pKa = 9.53IYY97 pKa = 10.58DD98 pKa = 4.01FSLPCPNTCDD108 pKa = 3.19TSSPLFVKK116 pKa = 10.67

Molecular weight: 12.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653WS13|A0A653WS13_9FLAO Uncharacterized protein OS=Flavobacterium sp. 9AF OX=2653142 GN=FLAVO9AF_70053 PE=4 SV=1
MM1 pKa = 7.28LRR3 pKa = 11.84WTITFIILAIVAGIFGFGGIAAGAASIAKK32 pKa = 9.28ILFFIFIVLFILSLISGRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84VV53 pKa = 3.18

Molecular weight: 5.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3815

0

3815

1261417

20

3515

330.6

37.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.722 ± 0.042

0.799 ± 0.014

5.132 ± 0.029

6.644 ± 0.049

5.539 ± 0.037

5.904 ± 0.051

1.651 ± 0.019

8.619 ± 0.04

8.255 ± 0.063

9.203 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.007 ± 0.02

7.153 ± 0.051

3.19 ± 0.029

3.506 ± 0.024

2.884 ± 0.024

6.574 ± 0.038

6.042 ± 0.052

5.784 ± 0.035

1.021 ± 0.013

4.372 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski