Amino acid dipepetide frequency for Lachnospiraceae bacterium MD308

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.51AlaAla: 7.51 ± 0.108	1.153AlaCys: 1.153 ± 0.037	4.536AlaAsp: 4.536 ± 0.059	5.656AlaGlu: 5.656 ± 0.08	2.996AlaPhe: 2.996 ± 0.054	6.358AlaGly: 6.358 ± 0.073	1.121AlaHis: 1.121 ± 0.031	4.528AlaIle: 4.528 ± 0.064	5.102AlaLys: 5.102 ± 0.065	6.574AlaLeu: 6.574 ± 0.081	2.168AlaMet: 2.168 ± 0.041	2.469AlaAsn: 2.469 ± 0.044	1.937AlaPro: 1.937 ± 0.042	2.151AlaGln: 2.151 ± 0.044	3.028AlaArg: 3.028 ± 0.054	3.767AlaSer: 3.767 ± 0.057	2.94AlaThr: 2.94 ± 0.06	6.586AlaVal: 6.586 ± 0.084	0.617AlaTrp: 0.617 ± 0.022	2.74AlaTyr: 2.74 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	1.096CysAla: 1.096 ± 0.027	0.301CysCys: 0.301 ± 0.016	0.824CysAsp: 0.824 ± 0.026	1.007CysGlu: 1.007 ± 0.03	0.685CysPhe: 0.685 ± 0.023	1.613CysGly: 1.613 ± 0.041	0.317CysHis: 0.317 ± 0.016	1.106CysIle: 1.106 ± 0.027	0.903CysLys: 0.903 ± 0.027	1.258CysLeu: 1.258 ± 0.036	0.502CysMet: 0.502 ± 0.021	0.571CysAsn: 0.571 ± 0.021	0.651CysPro: 0.651 ± 0.024	0.413CysGln: 0.413 ± 0.018	0.89CysArg: 0.89 ± 0.03	0.894CysSer: 0.894 ± 0.029	0.781CysThr: 0.781 ± 0.023	1.075CysVal: 1.075 ± 0.028	0.119CysTrp: 0.119 ± 0.01	0.626CysTyr: 0.626 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	4.185AspAla: 4.185 ± 0.055	0.825AspCys: 0.825 ± 0.025	2.829AspAsp: 2.829 ± 0.063	4.725AspGlu: 4.725 ± 0.066	2.648AspPhe: 2.648 ± 0.052	4.596AspGly: 4.596 ± 0.078	0.696AspHis: 0.696 ± 0.022	4.88AspIle: 4.88 ± 0.066	3.667AspLys: 3.667 ± 0.056	3.965AspLeu: 3.965 ± 0.063	1.918AspMet: 1.918 ± 0.039	2.195AspAsn: 2.195 ± 0.044	1.297AspPro: 1.297 ± 0.035	0.942AspGln: 0.942 ± 0.029	2.555AspArg: 2.555 ± 0.048	3.128AspSer: 3.128 ± 0.057	3.096AspThr: 3.096 ± 0.058	3.829AspVal: 3.829 ± 0.054	0.56AspTrp: 0.56 ± 0.023	2.822AspTyr: 2.822 ± 0.057	0.0AspXaa: 0.0 ± 0.0
Glu	5.193GluAla: 5.193 ± 0.079	0.939GluCys: 0.939 ± 0.027	4.496GluAsp: 4.496 ± 0.071	7.928GluGlu: 7.928 ± 0.141	2.75GluPhe: 2.75 ± 0.042	4.801GluGly: 4.801 ± 0.072	1.426GluHis: 1.426 ± 0.033	5.803GluIle: 5.803 ± 0.068	7.167GluLys: 7.167 ± 0.091	7.216GluLeu: 7.216 ± 0.067	2.544GluMet: 2.544 ± 0.048	4.042GluAsn: 4.042 ± 0.058	2.037GluPro: 2.037 ± 0.042	2.989GluGln: 2.989 ± 0.058	4.198GluArg: 4.198 ± 0.065	3.672GluSer: 3.672 ± 0.059	3.879GluThr: 3.879 ± 0.066	4.616GluVal: 4.616 ± 0.07	0.746GluTrp: 0.746 ± 0.024	3.433GluTyr: 3.433 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	3.01PheAla: 3.01 ± 0.051	0.766PheCys: 0.766 ± 0.025	2.507PheAsp: 2.507 ± 0.044	2.799PheGlu: 2.799 ± 0.047	1.838PhePhe: 1.838 ± 0.044	3.032PheGly: 3.032 ± 0.054	0.771PheHis: 0.771 ± 0.026	2.776PheIle: 2.776 ± 0.053	2.139PheLys: 2.139 ± 0.043	4.0PheLeu: 4.0 ± 0.066	1.169PheMet: 1.169 ± 0.028	1.447PheAsn: 1.447 ± 0.035	1.442PhePro: 1.442 ± 0.036	1.208PheGln: 1.208 ± 0.029	1.904PheArg: 1.904 ± 0.042	2.881PheSer: 2.881 ± 0.053	2.22PheThr: 2.22 ± 0.038	2.769PheVal: 2.769 ± 0.051	0.397PheTrp: 0.397 ± 0.021	1.782PheTyr: 1.782 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	5.123GlyAla: 5.123 ± 0.077	1.28GlyCys: 1.28 ± 0.033	3.516GlyAsp: 3.516 ± 0.06	5.293GlyGlu: 5.293 ± 0.062	3.077GlyPhe: 3.077 ± 0.052	5.14GlyGly: 5.14 ± 0.087	1.262GlyHis: 1.262 ± 0.034	6.129GlyIle: 6.129 ± 0.076	6.172GlyLys: 6.172 ± 0.084	5.679GlyLeu: 5.679 ± 0.076	2.614GlyMet: 2.614 ± 0.053	3.217GlyAsn: 3.217 ± 0.059	1.082GlyPro: 1.082 ± 0.033	2.154GlyGln: 2.154 ± 0.049	3.532GlyArg: 3.532 ± 0.058	3.959GlySer: 3.959 ± 0.057	4.089GlyThr: 4.089 ± 0.061	4.917GlyVal: 4.917 ± 0.075	0.693GlyTrp: 0.693 ± 0.021	3.33GlyTyr: 3.33 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	1.162HisAla: 1.162 ± 0.035	0.306HisCys: 0.306 ± 0.015	0.888HisAsp: 0.888 ± 0.025	1.107HisGlu: 1.107 ± 0.027	0.791HisPhe: 0.791 ± 0.026	1.262HisGly: 1.262 ± 0.033	0.385HisHis: 0.385 ± 0.021	1.327HisIle: 1.327 ± 0.034	0.97HisLys: 0.97 ± 0.027	1.473HisLeu: 1.473 ± 0.036	0.579HisMet: 0.579 ± 0.023	0.634HisAsn: 0.634 ± 0.019	0.835HisPro: 0.835 ± 0.028	0.49HisGln: 0.49 ± 0.021	0.845HisArg: 0.845 ± 0.027	0.937HisSer: 0.937 ± 0.03	0.964HisThr: 0.964 ± 0.029	1.097HisVal: 1.097 ± 0.03	0.154HisTrp: 0.154 ± 0.01	0.745HisTyr: 0.745 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.519IleAla: 5.519 ± 0.068	1.342IleCys: 1.342 ± 0.033	4.022IleAsp: 4.022 ± 0.059	4.966IleGlu: 4.966 ± 0.08	2.998IlePhe: 2.998 ± 0.057	4.954IleGly: 4.954 ± 0.073	1.299IleHis: 1.299 ± 0.031	4.941IleIle: 4.941 ± 0.076	4.399IleLys: 4.399 ± 0.057	6.667IleLeu: 6.667 ± 0.092	1.987IleMet: 1.987 ± 0.041	2.773IleAsn: 2.773 ± 0.05	3.018IlePro: 3.018 ± 0.044	2.16IleGln: 2.16 ± 0.036	3.827IleArg: 3.827 ± 0.06	4.64IleSer: 4.64 ± 0.066	3.896IleThr: 3.896 ± 0.057	4.816IleVal: 4.816 ± 0.064	0.672IleTrp: 0.672 ± 0.026	2.859IleTyr: 2.859 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	5.394LysAla: 5.394 ± 0.073	0.797LysCys: 0.797 ± 0.027	4.15LysAsp: 4.15 ± 0.065	7.44LysGlu: 7.44 ± 0.086	1.879LysPhe: 1.879 ± 0.04	4.672LysGly: 4.672 ± 0.069	0.958LysHis: 0.958 ± 0.027	5.056LysIle: 5.056 ± 0.077	7.079LysLys: 7.079 ± 0.104	5.613LysLeu: 5.613 ± 0.065	2.128LysMet: 2.128 ± 0.041	3.663LysAsn: 3.663 ± 0.055	2.026LysPro: 2.026 ± 0.044	2.299LysGln: 2.299 ± 0.049	3.597LysArg: 3.597 ± 0.063	3.829LysSer: 3.829 ± 0.059	3.864LysThr: 3.864 ± 0.061	4.739LysVal: 4.739 ± 0.077	0.664LysTrp: 0.664 ± 0.023	3.114LysTyr: 3.114 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	6.521LeuAla: 6.521 ± 0.086	1.558LeuCys: 1.558 ± 0.036	4.789LeuAsp: 4.789 ± 0.067	6.47LeuGlu: 6.47 ± 0.083	3.856LeuPhe: 3.856 ± 0.072	5.695LeuGly: 5.695 ± 0.072	1.477LeuHis: 1.477 ± 0.038	5.598LeuIle: 5.598 ± 0.078	6.686LeuLys: 6.686 ± 0.075	8.262LeuLeu: 8.262 ± 0.107	2.568LeuMet: 2.568 ± 0.046	3.749LeuAsn: 3.749 ± 0.053	3.352LeuPro: 3.352 ± 0.051	2.493LeuGln: 2.493 ± 0.048	4.112LeuArg: 4.112 ± 0.048	6.364LeuSer: 6.364 ± 0.068	4.712LeuThr: 4.712 ± 0.057	5.096LeuVal: 5.096 ± 0.07	0.731LeuTrp: 0.731 ± 0.026	3.449LeuTyr: 3.449 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	2.344MetAla: 2.344 ± 0.045	0.401MetCys: 0.401 ± 0.018	1.895MetAsp: 1.895 ± 0.043	2.69MetGlu: 2.69 ± 0.049	1.116MetPhe: 1.116 ± 0.029	2.215MetGly: 2.215 ± 0.043	0.468MetHis: 0.468 ± 0.02	2.011MetIle: 2.011 ± 0.043	2.511MetLys: 2.511 ± 0.045	2.854MetLeu: 2.854 ± 0.05	0.939MetMet: 0.939 ± 0.03	1.494MetAsn: 1.494 ± 0.034	1.115MetPro: 1.115 ± 0.034	1.054MetGln: 1.054 ± 0.029	1.45MetArg: 1.45 ± 0.035	1.849MetSer: 1.849 ± 0.04	1.643MetThr: 1.643 ± 0.036	1.929MetVal: 1.929 ± 0.039	0.235MetTrp: 0.235 ± 0.014	1.006MetTyr: 1.006 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	2.999AsnAla: 2.999 ± 0.046	0.652AsnCys: 0.652 ± 0.025	1.988AsnAsp: 1.988 ± 0.041	2.737AsnGlu: 2.737 ± 0.051	1.534AsnPhe: 1.534 ± 0.038	3.373AsnGly: 3.373 ± 0.059	0.809AsnHis: 0.809 ± 0.024	3.378AsnIle: 3.378 ± 0.053	2.589AsnLys: 2.589 ± 0.053	3.753AsnLeu: 3.753 ± 0.055	1.409AsnMet: 1.409 ± 0.033	1.829AsnAsn: 1.829 ± 0.054	1.98AsnPro: 1.98 ± 0.037	1.38AsnGln: 1.38 ± 0.034	2.148AsnArg: 2.148 ± 0.042	2.218AsnSer: 2.218 ± 0.05	2.219AsnThr: 2.219 ± 0.041	2.791AsnVal: 2.791 ± 0.051	0.413AsnTrp: 0.413 ± 0.017	1.8AsnTyr: 1.8 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	2.328ProAla: 2.328 ± 0.045	0.468ProCys: 0.468 ± 0.021	2.175ProAsp: 2.175 ± 0.048	3.329ProGlu: 3.329 ± 0.053	1.391ProPhe: 1.391 ± 0.032	2.308ProGly: 2.308 ± 0.049	0.54ProHis: 0.54 ± 0.021	1.825ProIle: 1.825 ± 0.042	2.001ProLys: 2.001 ± 0.048	2.586ProLeu: 2.586 ± 0.047	0.836ProMet: 0.836 ± 0.031	1.131ProAsn: 1.131 ± 0.032	0.759ProPro: 0.759 ± 0.031	1.02ProGln: 1.02 ± 0.027	1.001ProArg: 1.001 ± 0.034	1.686ProSer: 1.686 ± 0.038	1.364ProThr: 1.364 ± 0.035	2.899ProVal: 2.899 ± 0.046	0.301ProTrp: 0.301 ± 0.015	1.444ProTyr: 1.444 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	2.238GlnAla: 2.238 ± 0.045	0.394GlnCys: 0.394 ± 0.017	1.431GlnAsp: 1.431 ± 0.038	2.679GlnGlu: 2.679 ± 0.049	1.175GlnPhe: 1.175 ± 0.031	1.88GlnGly: 1.88 ± 0.044	0.414GlnHis: 0.414 ± 0.017	2.48GlnIle: 2.48 ± 0.048	2.606GlnLys: 2.606 ± 0.05	2.587GlnLeu: 2.587 ± 0.048	1.24GlnMet: 1.24 ± 0.032	1.439GlnAsn: 1.439 ± 0.036	0.803GlnPro: 0.803 ± 0.029	1.129GlnGln: 1.129 ± 0.04	1.527GlnArg: 1.527 ± 0.033	1.621GlnSer: 1.621 ± 0.035	1.545GlnThr: 1.545 ± 0.038	1.91GlnVal: 1.91 ± 0.038	0.308GlnTrp: 0.308 ± 0.014	1.344GlnTyr: 1.344 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.981ArgAla: 2.981 ± 0.049	0.688ArgCys: 0.688 ± 0.025	2.366ArgAsp: 2.366 ± 0.05	4.327ArgGlu: 4.327 ± 0.065	2.02ArgPhe: 2.02 ± 0.041	2.762ArgGly: 2.762 ± 0.052	0.914ArgHis: 0.914 ± 0.029	3.821ArgIle: 3.821 ± 0.062	3.921ArgLys: 3.921 ± 0.054	4.308ArgLeu: 4.308 ± 0.067	1.746ArgMet: 1.746 ± 0.043	2.165ArgAsn: 2.165 ± 0.039	1.362ArgPro: 1.362 ± 0.038	1.926ArgGln: 1.926 ± 0.043	2.861ArgArg: 2.861 ± 0.058	2.176ArgSer: 2.176 ± 0.041	2.404ArgThr: 2.404 ± 0.044	2.823ArgVal: 2.823 ± 0.048	0.437ArgTrp: 0.437 ± 0.019	2.156ArgTyr: 2.156 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	4.2SerAla: 4.2 ± 0.066	0.827SerCys: 0.827 ± 0.026	3.255SerAsp: 3.255 ± 0.052	4.241SerGlu: 4.241 ± 0.061	2.609SerPhe: 2.609 ± 0.041	5.201SerGly: 5.201 ± 0.071	1.079SerHis: 1.079 ± 0.032	3.85SerIle: 3.85 ± 0.06	3.534SerLys: 3.534 ± 0.062	5.049SerLeu: 5.049 ± 0.071	1.829SerMet: 1.829 ± 0.04	2.213SerAsn: 2.213 ± 0.045	1.711SerPro: 1.711 ± 0.039	1.778SerGln: 1.778 ± 0.039	2.78SerArg: 2.78 ± 0.047	3.498SerSer: 3.498 ± 0.072	2.41SerThr: 2.41 ± 0.048	4.521SerVal: 4.521 ± 0.065	0.577SerTrp: 0.577 ± 0.025	2.45SerTyr: 2.45 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	4.383ThrAla: 4.383 ± 0.068	0.681ThrCys: 0.681 ± 0.024	3.098ThrAsp: 3.098 ± 0.049	3.895ThrGlu: 3.895 ± 0.058	2.05ThrPhe: 2.05 ± 0.037	4.479ThrGly: 4.479 ± 0.072	0.833ThrHis: 0.833 ± 0.026	3.49ThrIle: 3.49 ± 0.064	3.181ThrLys: 3.181 ± 0.059	4.426ThrLeu: 4.426 ± 0.057	1.358ThrMet: 1.358 ± 0.03	1.816ThrAsn: 1.816 ± 0.046	1.904ThrPro: 1.904 ± 0.044	1.422ThrGln: 1.422 ± 0.04	1.919ThrArg: 1.919 ± 0.04	2.759ThrSer: 2.759 ± 0.054	2.431ThrThr: 2.431 ± 0.05	4.606ThrVal: 4.606 ± 0.07	0.445ThrTrp: 0.445 ± 0.018	2.119ThrTyr: 2.119 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	4.421ValAla: 4.421 ± 0.074	1.332ValCys: 1.332 ± 0.032	3.503ValAsp: 3.503 ± 0.061	4.632ValGlu: 4.632 ± 0.061	3.15ValPhe: 3.15 ± 0.046	4.171ValGly: 4.171 ± 0.068	1.126ValHis: 1.126 ± 0.031	5.129ValIle: 5.129 ± 0.066	5.089ValLys: 5.089 ± 0.079	6.635ValLeu: 6.635 ± 0.074	2.141ValMet: 2.141 ± 0.037	2.776ValAsn: 2.776 ± 0.045	2.452ValPro: 2.452 ± 0.046	1.951ValGln: 1.951 ± 0.042	3.237ValArg: 3.237 ± 0.054	4.819ValSer: 4.819 ± 0.059	4.137ValThr: 4.137 ± 0.069	4.578ValVal: 4.578 ± 0.081	0.69ValTrp: 0.69 ± 0.024	2.803ValTyr: 2.803 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.529TrpAla: 0.529 ± 0.023	0.176TrpCys: 0.176 ± 0.012	0.512TrpAsp: 0.512 ± 0.021	0.765TrpGlu: 0.765 ± 0.027	0.432TrpPhe: 0.432 ± 0.019	0.656TrpGly: 0.656 ± 0.025	0.17TrpHis: 0.17 ± 0.012	0.637TrpIle: 0.637 ± 0.024	0.772TrpLys: 0.772 ± 0.024	0.86TrpLeu: 0.86 ± 0.029	0.316TrpMet: 0.316 ± 0.017	0.533TrpAsn: 0.533 ± 0.024	0.199TrpPro: 0.199 ± 0.013	0.326TrpGln: 0.326 ± 0.017	0.453TrpArg: 0.453 ± 0.02	0.517TrpSer: 0.517 ± 0.02	0.426TrpThr: 0.426 ± 0.018	0.527TrpVal: 0.527 ± 0.02	0.141TrpTrp: 0.141 ± 0.011	0.372TrpTyr: 0.372 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.8TyrAla: 2.8 ± 0.046	0.745TyrCys: 0.745 ± 0.026	2.578TyrAsp: 2.578 ± 0.051	3.19TyrGlu: 3.19 ± 0.056	1.861TyrPhe: 1.861 ± 0.044	3.089TyrGly: 3.089 ± 0.051	0.883TyrHis: 0.883 ± 0.027	2.988TyrIle: 2.988 ± 0.046	2.475TyrLys: 2.475 ± 0.043	3.775TyrLeu: 3.775 ± 0.061	1.228TyrMet: 1.228 ± 0.03	1.843TyrAsn: 1.843 ± 0.041	1.429TyrPro: 1.429 ± 0.035	1.465TyrGln: 1.465 ± 0.035	2.303TyrArg: 2.303 ± 0.049	2.396TyrSer: 2.396 ± 0.047	2.274TyrThr: 2.274 ± 0.046	2.651TyrVal: 2.651 ± 0.048	0.419TyrTrp: 0.419 ± 0.017	1.97TyrTyr: 1.97 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4125 proteins (1278572 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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