Polaribacter sp. KT25b

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Polaribacter; unclassified Polaribacter

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3350 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1NZM2|A0A1H1NZM2_9FLAO Histidine phosphatase superfamily (Branch 1) OS=Polaribacter sp. KT25b OX=1855336 GN=SAMN05216503_1775 PE=4 SV=1
MM1 pKa = 7.36TKK3 pKa = 9.84IKK5 pKa = 10.65NILALIILATIVYY18 pKa = 9.87ACDD21 pKa = 3.51EE22 pKa = 4.33SSSVYY27 pKa = 10.45VDD29 pKa = 3.05NFDD32 pKa = 4.28YY33 pKa = 10.93EE34 pKa = 4.44GQALIDD40 pKa = 3.66SDD42 pKa = 4.28TLVKK46 pKa = 10.37FLSNHH51 pKa = 5.85YY52 pKa = 10.63YY53 pKa = 10.74DD54 pKa = 3.62VTEE57 pKa = 5.16DD58 pKa = 3.61EE59 pKa = 4.66VKK61 pKa = 10.94LLVSGKK67 pKa = 7.71TALIEE72 pKa = 4.57DD73 pKa = 4.55EE74 pKa = 5.12NLEE77 pKa = 4.15QYY79 pKa = 11.16DD80 pKa = 3.96VVDD83 pKa = 3.85NEE85 pKa = 3.71IDD87 pKa = 3.55YY88 pKa = 9.29TFYY91 pKa = 11.19YY92 pKa = 7.85YY93 pKa = 10.37TNRR96 pKa = 11.84VGSPTVEE103 pKa = 3.57KK104 pKa = 10.63GYY106 pKa = 8.3PTVMDD111 pKa = 3.93SVLVKK116 pKa = 10.73YY117 pKa = 9.86EE118 pKa = 3.76GKK120 pKa = 10.63RR121 pKa = 11.84IVNTDD126 pKa = 3.72SISASFDD133 pKa = 3.46KK134 pKa = 11.51NNGLWLTLNSVIRR147 pKa = 11.84GWTYY151 pKa = 10.2GIPKK155 pKa = 10.13FKK157 pKa = 10.9GGDD160 pKa = 3.52NITDD164 pKa = 3.77NGPITYY170 pKa = 9.97EE171 pKa = 3.88NGGKK175 pKa = 10.53GILFLPSGLAYY186 pKa = 10.16GPSGTTSILGSEE198 pKa = 4.14CLMFKK203 pKa = 10.29IEE205 pKa = 5.07LYY207 pKa = 11.04DD208 pKa = 4.26FIKK211 pKa = 10.54DD212 pKa = 3.46TDD214 pKa = 3.72HH215 pKa = 7.57DD216 pKa = 4.33NDD218 pKa = 3.77GVASFFEE225 pKa = 5.21DD226 pKa = 3.87PDD228 pKa = 5.02GDD230 pKa = 3.9GDD232 pKa = 4.44PRR234 pKa = 11.84NDD236 pKa = 3.71DD237 pKa = 3.52TDD239 pKa = 5.26LDD241 pKa = 4.14GSPNYY246 pKa = 10.8ADD248 pKa = 5.37ADD250 pKa = 4.09DD251 pKa = 5.71DD252 pKa = 4.92GDD254 pKa = 4.86GILTINEE261 pKa = 4.24DD262 pKa = 4.01ANSDD266 pKa = 3.77GNPANDD272 pKa = 4.89FSDD275 pKa = 4.18PDD277 pKa = 3.61NSTLADD283 pKa = 3.84YY284 pKa = 11.07LNSLIKK290 pKa = 10.73VSNN293 pKa = 3.62

Molecular weight: 32.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1RSI1|A0A1H1RSI1_9FLAO Uncharacterized protein OS=Polaribacter sp. KT25b OX=1855336 GN=SAMN05216503_2899 PE=4 SV=1
MM1 pKa = 7.66ASLIGHH7 pKa = 7.75AFTSIALGKK16 pKa = 10.04SFSRR20 pKa = 11.84NRR22 pKa = 11.84RR23 pKa = 11.84NWKK26 pKa = 9.89LILIAIVCAIIQDD39 pKa = 3.1IDD41 pKa = 3.9VIGFQFGIPYY51 pKa = 10.22NSFWGHH57 pKa = 6.29RR58 pKa = 11.84GFTHH62 pKa = 6.91SLFFALLLAILVTAIFYY79 pKa = 10.41KK80 pKa = 10.8KK81 pKa = 10.61LFFSKK86 pKa = 10.34KK87 pKa = 10.2GIILILFFFLCGAFHH102 pKa = 7.39SILDD106 pKa = 3.75AFTDD110 pKa = 3.85GGNGVAFFTPFNNTRR125 pKa = 11.84YY126 pKa = 8.83FFPWRR131 pKa = 11.84PIKK134 pKa = 10.48ASPLGITRR142 pKa = 11.84FFSEE146 pKa = 3.98RR147 pKa = 11.84GMLVIRR153 pKa = 11.84SEE155 pKa = 4.4VTWIVLPGFIYY166 pKa = 9.62MVIAFLIRR174 pKa = 11.84KK175 pKa = 8.54KK176 pKa = 10.51RR177 pKa = 11.84NKK179 pKa = 9.51II180 pKa = 3.38

Molecular weight: 20.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3350

0

3350

1171335

29

3002

349.7

39.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.899 ± 0.036

0.678 ± 0.012

5.502 ± 0.035

6.602 ± 0.037

5.532 ± 0.039

5.995 ± 0.039

1.602 ± 0.017

8.566 ± 0.043

8.864 ± 0.055

9.167 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.984 ± 0.022

6.824 ± 0.045

3.068 ± 0.023

3.09 ± 0.02

2.997 ± 0.022

6.68 ± 0.039

5.946 ± 0.042

5.949 ± 0.031

1.031 ± 0.015

4.023 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski