Enterococcus phage vB_EfaS_IME196

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Efquatrovirus; Enterococcus virus IME196

Average proteome isoelectric point is 5.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2MYB4|A0A0S2MYB4_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaS_IME196 OX=1747289 PE=4 SV=1
MM1 pKa = 6.88MTTKK5 pKa = 10.14TNYY8 pKa = 10.03HH9 pKa = 5.89ICLTNNEE16 pKa = 4.26YY17 pKa = 10.96FNLYY21 pKa = 8.63TEE23 pKa = 4.36EE24 pKa = 5.61PILVMYY30 pKa = 10.14EE31 pKa = 3.76EE32 pKa = 4.92AVEE35 pKa = 4.03NDD37 pKa = 3.34EE38 pKa = 6.02KK39 pKa = 11.25LLKK42 pKa = 10.07LDD44 pKa = 3.68KK45 pKa = 11.12PEE47 pKa = 5.63DD48 pKa = 3.61IEE50 pKa = 5.76IDD52 pKa = 3.67GEE54 pKa = 4.27MQPTFITIPLDD65 pKa = 3.76SILYY69 pKa = 8.75VLEE72 pKa = 4.42DD73 pKa = 3.32ARR75 pKa = 5.3

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2MY79|A0A0S2MY79_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaS_IME196 OX=1747289 PE=4 SV=1
MM1 pKa = 7.62ILKK4 pKa = 9.98LLNWRR9 pKa = 11.84QFKK12 pKa = 9.43MALFGLVFVTIGIWGTYY29 pKa = 10.29NIIKK33 pKa = 10.09YY34 pKa = 10.23IEE36 pKa = 4.27DD37 pKa = 3.3

Molecular weight: 4.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

11321

37

1456

198.6

22.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.033 ± 0.405

0.512 ± 0.103

6.166 ± 0.241

8.109 ± 0.541

4.302 ± 0.245

6.351 ± 0.784

1.528 ± 0.174

6.669 ± 0.273

8.745 ± 0.367

8.391 ± 0.268

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.8 ± 0.141

6.581 ± 0.351

3.092 ± 0.226

3.701 ± 0.21

3.357 ± 0.17

5.335 ± 0.263

6.13 ± 0.37

6.81 ± 0.254

1.325 ± 0.111

4.063 ± 0.424
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski