Hubei toti-like virus 10
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KF66|A0A1L3KF66_9VIRU Uncharacterized protein OS=Hubei toti-like virus 10 OX=1923298 PE=4 SV=1
MM1 pKa = 7.74 VITRR5 pKa = 11.84 QQAKK9 pKa = 7.71 TKK11 pKa = 10.12 EE12 pKa = 4.2 MNGQQDD18 pKa = 3.21 MALTPKK24 pKa = 9.88 VGRR27 pKa = 11.84 GRR29 pKa = 11.84 GVPKK33 pKa = 10.69 ALTTPVPKK41 pKa = 10.08 QASGHH46 pKa = 6.08 PNGVSDD52 pKa = 5.91 AEE54 pKa = 4.15 AKK56 pKa = 9.11 MQEE59 pKa = 4.18 KK60 pKa = 10.62 VMEE63 pKa = 4.23 STTAKK68 pKa = 10.36 VGEE71 pKa = 4.38 LVLIAPCVGVPPGDD85 pKa = 3.68 VAVVDD90 pKa = 4.86 ASSSTLAPTASGPSNTGGEE109 pKa = 4.2 EE110 pKa = 4.04 MLVGTVVEE118 pKa = 4.37 PMDD121 pKa = 4.0 VSGTVNDD128 pKa = 4.09 MPVDD132 pKa = 4.07 ADD134 pKa = 3.75 SQAAGPSSVRR144 pKa = 11.84 GPVSSASLGGASGGSVPSTSATGSVLSIAANAVVSGAPAVRR185 pKa = 11.84 YY186 pKa = 8.26 PGNSASRR193 pKa = 11.84 PRR195 pKa = 11.84 VAPLAMSGVPVPATPFTGEE214 pKa = 3.77 YY215 pKa = 9.83 HH216 pKa = 6.44 QVDD219 pKa = 4.48 DD220 pKa = 4.23 LQKK223 pKa = 9.22 IGRR226 pKa = 11.84 SSVVPNNVVAFPVGSCPEE244 pKa = 3.82 QTQTILAGLRR254 pKa = 11.84 KK255 pKa = 9.68 LLTAQPEE262 pKa = 4.4 RR263 pKa = 11.84 IDD265 pKa = 3.48 VQEE268 pKa = 4.23 RR269 pKa = 11.84 EE270 pKa = 4.06 IVITGIVSMPPLRR283 pKa = 11.84 GDD285 pKa = 3.2 APMARR290 pKa = 11.84 VTTHH294 pKa = 6.46 FNRR297 pKa = 11.84 TYY299 pKa = 10.82 PEE301 pKa = 4.37 DD302 pKa = 3.84 APTRR306 pKa = 11.84 RR307 pKa = 11.84 TQLTFAPPVIIGEE320 pKa = 4.14 VDD322 pKa = 3.29 PAIQAQYY329 pKa = 10.53 IKK331 pKa = 11.25 NNLAEE336 pKa = 4.17 FRR338 pKa = 11.84 PTSSALRR345 pKa = 11.84 GQVIMEE351 pKa = 3.7 MTMANRR357 pKa = 11.84 ITTLLSEE364 pKa = 4.72 AAQDD368 pKa = 3.7 FDD370 pKa = 5.66 LMGLFTIIHH379 pKa = 6.24 LMVDD383 pKa = 3.08 YY384 pKa = 10.95 LYY386 pKa = 10.56 FLQRR390 pKa = 11.84 LGINAVVAANQPDD403 pKa = 4.36 YY404 pKa = 11.24 IAFINVNPPAGDD416 pKa = 2.91 IAAATNEE423 pKa = 3.92 VTNAVMQGKK432 pKa = 9.3 LCFDD436 pKa = 3.9 NRR438 pKa = 11.84 WVSVQDD444 pKa = 3.8 MRR446 pKa = 11.84 CMMAVSAGPAYY457 pKa = 10.56 FNIGAPANVPIHH469 pKa = 6.28 HH470 pKa = 7.39 NIRR473 pKa = 11.84 TPPIYY478 pKa = 9.52 WALWDD483 pKa = 3.94 NAPIVNPGPGMVSISDD499 pKa = 3.86 LLAFAEE505 pKa = 4.68 KK506 pKa = 10.32 VVKK509 pKa = 10.32 HH510 pKa = 5.06 YY511 pKa = 10.53 SCRR514 pKa = 11.84 HH515 pKa = 4.57 AYY517 pKa = 9.22 VRR519 pKa = 11.84 GFIRR523 pKa = 11.84 ASMLINGMVEE533 pKa = 3.99 VIGVGNHH540 pKa = 6.03 IYY542 pKa = 9.95 MSSCLEE548 pKa = 3.81 FEE550 pKa = 4.54 RR551 pKa = 11.84 FSVPRR556 pKa = 11.84 PRR558 pKa = 11.84 GRR560 pKa = 11.84 NMVWMLLQAVWTSTINPLFFEE581 pKa = 4.85 AEE583 pKa = 4.13 FFALAGLQVAGMVQLGAAVAAMVSLATSTVFHH615 pKa = 6.99 HH616 pKa = 6.65 FNITGRR622 pKa = 11.84 EE623 pKa = 3.84 LTMWAGRR630 pKa = 11.84 DD631 pKa = 3.56 PALSTHH637 pKa = 6.95 VINQLFYY644 pKa = 8.45 PASGRR649 pKa = 11.84 EE650 pKa = 4.0 CTLLHH655 pKa = 6.39 MSTTLVVAAFSGMSIHH671 pKa = 6.21 WRR673 pKa = 11.84 CFSTTSWSNGFEE685 pKa = 4.06 HH686 pKa = 7.44 RR687 pKa = 11.84 VMEE690 pKa = 5.8 LNDD693 pKa = 3.17 GWQHH697 pKa = 4.32 IWHH700 pKa = 6.56 RR701 pKa = 11.84 HH702 pKa = 3.36 VPYY705 pKa = 10.05 PVLVLSMEE713 pKa = 3.97 WAVYY717 pKa = 10.09 SWILEE722 pKa = 4.03 WGYY725 pKa = 11.15 SGPNPVYY732 pKa = 11.35 NMGNEE737 pKa = 4.02 MFVAGPAASRR747 pKa = 11.84 GVYY750 pKa = 8.18 VHH752 pKa = 6.92 EE753 pKa = 4.66 GDD755 pKa = 3.36 IKK757 pKa = 10.92 YY758 pKa = 10.21 VQATQSQSTFVYY770 pKa = 9.54 QVYY773 pKa = 9.82 GALFINTVRR782 pKa = 11.84 GDD784 pKa = 3.64 WRR786 pKa = 11.84 CGDD789 pKa = 3.57 AMPISFQTISRR800 pKa = 11.84 SDD802 pKa = 3.68 SGHH805 pKa = 5.85 VIYY808 pKa = 10.56 GRR810 pKa = 11.84 EE811 pKa = 4.07 LLRR814 pKa = 11.84 EE815 pKa = 4.51 DD816 pKa = 5.32 DD817 pKa = 4.88 LQPLYY822 pKa = 11.04 NVDD825 pKa = 3.14 IFAILPGTLISYY837 pKa = 10.11 DD838 pKa = 3.01 WVRR841 pKa = 11.84 NCTLGPVLLASDD853 pKa = 3.47 MDD855 pKa = 4.17 GDD857 pKa = 4.75 VFQNWLRR864 pKa = 11.84 NSRR867 pKa = 11.84 TDD869 pKa = 3.32 VVSAAGFSTRR879 pKa = 11.84 FAPQLGEE886 pKa = 3.74 IAITQFDD893 pKa = 4.04 MSSLLFGAGGNKK905 pKa = 8.44 QAAGASMDD913 pKa = 4.76 DD914 pKa = 3.86 SAQEE918 pKa = 4.2 GNN920 pKa = 3.58
Molecular weight: 99.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.525
IPC2_protein 5.474
IPC_protein 5.512
Toseland 5.881
ProMoST 5.817
Dawson 5.728
Bjellqvist 5.753
Wikipedia 5.715
Rodwell 5.69
Grimsley 6.02
Solomon 5.728
Lehninger 5.715
Nozaki 5.97
DTASelect 6.16
Thurlkill 6.122
EMBOSS 6.084
Sillero 6.059
Patrickios 3.973
IPC_peptide 5.741
IPC2_peptide 6.084
IPC2.peptide.svr19 5.981
Protein with the highest isoelectric point:
>tr|A0A1L3KF66|A0A1L3KF66_9VIRU Uncharacterized protein OS=Hubei toti-like virus 10 OX=1923298 PE=4 SV=1
MM1 pKa = 8.06 DD2 pKa = 4.44 EE3 pKa = 4.63 SGLICRR9 pKa = 11.84 VCEE12 pKa = 3.96 RR13 pKa = 11.84 VNVTCDD19 pKa = 2.85 KK20 pKa = 10.75 KK21 pKa = 10.7 AAGPVRR27 pKa = 11.84 LRR29 pKa = 11.84 PGDD32 pKa = 3.38 VSSIVVSPIYY42 pKa = 10.22 LKK44 pKa = 10.73 PHH46 pKa = 5.69 TCLLSTLRR54 pKa = 11.84 EE55 pKa = 4.17 IEE57 pKa = 4.15 WYY59 pKa = 10.16 KK60 pKa = 10.57 HH61 pKa = 4.4 GKK63 pKa = 9.53 LMSEE67 pKa = 4.21 EE68 pKa = 5.69 LYY70 pKa = 11.1 DD71 pKa = 3.12 EE72 pKa = 4.32 WFNVNSDD79 pKa = 3.57 NYY81 pKa = 10.78 QLGASKK87 pKa = 10.79 KK88 pKa = 10.29 CMQLWGDD95 pKa = 3.45 FTKK98 pKa = 10.75 CVIQRR103 pKa = 11.84 SPVEE107 pKa = 4.02 SVGISDD113 pKa = 4.59 LCVNCVVDD121 pKa = 5.03 LCLCKK126 pKa = 10.33 KK127 pKa = 10.19 HH128 pKa = 6.05 FGYY131 pKa = 10.61 YY132 pKa = 10.34 VSINWGRR139 pKa = 11.84 VNKK142 pKa = 10.57 RR143 pKa = 11.84 GDD145 pKa = 3.24 IANLNEE151 pKa = 3.21 IWQWSSAADD160 pKa = 3.31 KK161 pKa = 11.36 GKK163 pKa = 9.09 TNVSAADD170 pKa = 4.92 LIWHH174 pKa = 5.66 MRR176 pKa = 11.84 RR177 pKa = 11.84 YY178 pKa = 9.52 PEE180 pKa = 4.04 LRR182 pKa = 11.84 ARR184 pKa = 11.84 FDD186 pKa = 2.74 KK187 pKa = 10.65 WSFLFSIRR195 pKa = 11.84 LSNVTITSLLIHH207 pKa = 6.94 LIGNPSHH214 pKa = 6.63 PRR216 pKa = 11.84 VFEE219 pKa = 3.96 LLDD222 pKa = 4.11 RR223 pKa = 11.84 YY224 pKa = 10.41 DD225 pKa = 3.8 YY226 pKa = 11.47 CCLDD230 pKa = 3.46 SASYY234 pKa = 8.75 STLLKK239 pKa = 10.34 NLSVALRR246 pKa = 11.84 RR247 pKa = 11.84 TAHH250 pKa = 6.3 WPDD253 pKa = 3.55 GSPATLEE260 pKa = 4.45 EE261 pKa = 4.38 VTQCSGWEE269 pKa = 3.91 LAVGRR274 pKa = 11.84 SEE276 pKa = 5.37 NRR278 pKa = 11.84 SDD280 pKa = 3.16 WAEE283 pKa = 4.03 EE284 pKa = 3.66 EE285 pKa = 4.48 SKK287 pKa = 9.86 RR288 pKa = 11.84 TNQVVHH294 pKa = 6.76 LGLPTHH300 pKa = 5.89 EE301 pKa = 4.87 RR302 pKa = 11.84 KK303 pKa = 9.92 NDD305 pKa = 3.51 VTNSVYY311 pKa = 10.47 CALLEE316 pKa = 4.32 EE317 pKa = 4.62 EE318 pKa = 4.95 LTTIMSEE325 pKa = 4.06 LVTAPRR331 pKa = 11.84 RR332 pKa = 11.84 SPSWKK337 pKa = 10.06 DD338 pKa = 3.38 YY339 pKa = 11.17 VEE341 pKa = 4.31 TRR343 pKa = 11.84 QVWVSSGSTGGKK355 pKa = 8.01 RR356 pKa = 11.84 MKK358 pKa = 10.43 MNDD361 pKa = 3.21 GTVSRR366 pKa = 11.84 VNKK369 pKa = 9.48 HH370 pKa = 6.27 AYY372 pKa = 9.75 FEE374 pKa = 4.59 SLTTEE379 pKa = 4.11 EE380 pKa = 4.07 MVAWMAEE387 pKa = 4.06 PPRR390 pKa = 11.84 IQATASEE397 pKa = 4.62 KK398 pKa = 11.1 YY399 pKa = 10.54 EE400 pKa = 3.91 MGKK403 pKa = 10.1 ARR405 pKa = 11.84 AIYY408 pKa = 8.3 GTEE411 pKa = 3.81 PVDD414 pKa = 3.74 YY415 pKa = 10.38 AISSYY420 pKa = 11.4 VLDD423 pKa = 5.53 DD424 pKa = 5.21 IEE426 pKa = 4.2 DD427 pKa = 3.38 HH428 pKa = 6.74 LYY430 pKa = 10.82 NVDD433 pKa = 4.17 GIEE436 pKa = 4.85 SGLTDD441 pKa = 3.58 MDD443 pKa = 4.19 FLVAMIRR450 pKa = 11.84 RR451 pKa = 11.84 LRR453 pKa = 11.84 KK454 pKa = 8.74 VEE456 pKa = 4.02 SSEE459 pKa = 4.41 VEE461 pKa = 4.02 CTMIDD466 pKa = 3.53 YY467 pKa = 11.22 SDD469 pKa = 4.81 FNYY472 pKa = 10.33 QHH474 pKa = 6.12 TLEE477 pKa = 4.37 AQSLVFKK484 pKa = 10.57 CLSARR489 pKa = 11.84 LRR491 pKa = 11.84 TMGHH495 pKa = 6.6 HH496 pKa = 7.34 DD497 pKa = 4.7 DD498 pKa = 5.55 KK499 pKa = 11.63 VAACDD504 pKa = 3.42 WVAAAMLNQWCKK516 pKa = 9.44 FPGVKK521 pKa = 9.33 RR522 pKa = 11.84 VAQRR526 pKa = 11.84 VVQGMFSGVRR536 pKa = 11.84 GTNFINTTLNVGYY549 pKa = 10.46 YY550 pKa = 9.14 RR551 pKa = 11.84 VARR554 pKa = 11.84 RR555 pKa = 11.84 WVSEE559 pKa = 3.7 NLNLRR564 pKa = 11.84 PTDD567 pKa = 3.68 EE568 pKa = 4.8 FNLHH572 pKa = 6.12 QGDD575 pKa = 4.94 DD576 pKa = 3.44 VWISNKK582 pKa = 9.58 SRR584 pKa = 11.84 LWAMAMYY591 pKa = 10.64 EE592 pKa = 4.34 SMQSTGLIFQPSKK605 pKa = 11.36 QMFDD609 pKa = 3.14 TSRR612 pKa = 11.84 GEE614 pKa = 3.92 FLRR617 pKa = 11.84 VVYY620 pKa = 9.26 TSKK623 pKa = 11.05 GCQGYY628 pKa = 8.53 LARR631 pKa = 11.84 AVGTLIVKK639 pKa = 8.24 PIQSTEE645 pKa = 3.84 VVAPAEE651 pKa = 4.06 RR652 pKa = 11.84 AHH654 pKa = 6.59 ALSSQIAILHH664 pKa = 5.35 RR665 pKa = 11.84 RR666 pKa = 11.84 GYY668 pKa = 8.19 TEE670 pKa = 4.44 EE671 pKa = 4.23 GSKK674 pKa = 10.3 VLWEE678 pKa = 4.07 AVVPYY683 pKa = 10.17 AARR686 pKa = 11.84 AKK688 pKa = 10.01 LVSGALTIPVGALLKK703 pKa = 10.82 SSLDD707 pKa = 3.41 NGLDD711 pKa = 2.87 IGPPMTAAGPSDD723 pKa = 4.31 KK724 pKa = 10.64 IKK726 pKa = 10.61 AIPSMVLSSKK736 pKa = 10.7 ALEE739 pKa = 4.15 EE740 pKa = 4.43 SVPSHH745 pKa = 6.69 MSTDD749 pKa = 3.11 WAKK752 pKa = 10.54 VMSRR756 pKa = 11.84 RR757 pKa = 11.84 LQSSFNYY764 pKa = 10.2 DD765 pKa = 3.63 SIVEE769 pKa = 4.32 ALHH772 pKa = 5.61 STNVTDD778 pKa = 5.44 SLRR781 pKa = 11.84 QEE783 pKa = 4.93 DD784 pKa = 4.49 RR785 pKa = 11.84 IKK787 pKa = 10.61 CLRR790 pKa = 11.84 VHH792 pKa = 6.33 EE793 pKa = 4.65 RR794 pKa = 11.84 DD795 pKa = 3.22 LRR797 pKa = 11.84 SWLEE801 pKa = 3.65 RR802 pKa = 11.84 LKK804 pKa = 10.86 PGTVRR809 pKa = 11.84 RR810 pKa = 11.84 DD811 pKa = 3.03 RR812 pKa = 11.84 RR813 pKa = 11.84 RR814 pKa = 11.84 YY815 pKa = 8.67 DD816 pKa = 3.1 ALMDD820 pKa = 4.33 GEE822 pKa = 4.38 SADD825 pKa = 4.83 AGFEE829 pKa = 4.3 DD830 pKa = 5.61 LLDD833 pKa = 4.32 TIARR837 pKa = 11.84 GVLGKK842 pKa = 9.0 RR843 pKa = 11.84 TQDD846 pKa = 2.87 SRR848 pKa = 11.84 GVMGCIMAAVSSSPYY863 pKa = 9.92 RR864 pKa = 11.84 SLPNAKK870 pKa = 9.74 LATGLPTLSAATVAIAASPYY890 pKa = 9.99 PDD892 pKa = 5.14 LSMQAASHH900 pKa = 5.9 IASLRR905 pKa = 11.84 RR906 pKa = 11.84 YY907 pKa = 9.9 INDD910 pKa = 3.31 SQLCDD915 pKa = 3.35 ILDD918 pKa = 4.1 GLSAGANQFEE928 pKa = 4.93 CEE930 pKa = 4.18 YY931 pKa = 10.79 HH932 pKa = 6.09 PVILSWIEE940 pKa = 3.8 KK941 pKa = 9.63 KK942 pKa = 10.84 ALDD945 pKa = 4.08 RR946 pKa = 11.84 MLTGIMTGGIVVKK959 pKa = 10.64 DD960 pKa = 4.36 DD961 pKa = 3.63 LRR963 pKa = 11.84 QTLVDD968 pKa = 3.16 WMRR971 pKa = 11.84 TYY973 pKa = 10.85 VRR975 pKa = 11.84 SLNKK979 pKa = 9.85 FPVFKK984 pKa = 10.72 QISKK988 pKa = 10.09 YY989 pKa = 10.68
Molecular weight: 111.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.823
IPC2_protein 7.117
IPC_protein 6.971
Toseland 6.766
ProMoST 7.614
Dawson 7.644
Bjellqvist 8.053
Wikipedia 7.556
Rodwell 7.658
Grimsley 6.839
Solomon 7.658
Lehninger 7.688
Nozaki 8.287
DTASelect 7.834
Thurlkill 7.878
EMBOSS 7.878
Sillero 8.185
Patrickios 4.266
IPC_peptide 7.658
IPC2_peptide 7.307
IPC2.peptide.svr19 7.152
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1909
920
989
954.5
105.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.853 ± 0.95
1.729 ± 0.447
5.186 ± 0.584
4.924 ± 0.628
3.038 ± 0.533
6.862 ± 0.898
2.2 ± 0.018
4.872 ± 0.165
3.405 ± 1.16
8.172 ± 0.922
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.667 ± 0.399
4.086 ± 0.334
5.081 ± 1.079
3.457 ± 0.469
6.129 ± 0.786
8.591 ± 0.608
5.657 ± 0.223
8.905 ± 0.687
2.095 ± 0.248
3.091 ± 0.336
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here