Amino acid dipepetide frequency for Rhodobiaceae bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.091AlaAla: 14.091 ± 0.149
0.956AlaCys: 0.956 ± 0.029
7.087AlaAsp: 7.087 ± 0.102
7.527AlaGlu: 7.527 ± 0.097
4.318AlaPhe: 4.318 ± 0.064
9.489AlaGly: 9.489 ± 0.133
2.219AlaHis: 2.219 ± 0.05
5.714AlaIle: 5.714 ± 0.075
4.121AlaLys: 4.121 ± 0.083
11.838AlaLeu: 11.838 ± 0.118
3.034AlaMet: 3.034 ± 0.065
3.164AlaAsn: 3.164 ± 0.057
5.043AlaPro: 5.043 ± 0.068
3.609AlaGln: 3.609 ± 0.062
6.701AlaArg: 6.701 ± 0.08
6.322AlaSer: 6.322 ± 0.091
6.061AlaThr: 6.061 ± 0.099
7.613AlaVal: 7.613 ± 0.097
1.357AlaTrp: 1.357 ± 0.038
2.641AlaTyr: 2.641 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
0.904CysAla: 0.904 ± 0.026
0.113CysCys: 0.113 ± 0.011
0.569CysAsp: 0.569 ± 0.023
0.442CysGlu: 0.442 ± 0.02
0.332CysPhe: 0.332 ± 0.019
0.812CysGly: 0.812 ± 0.026
0.219CysHis: 0.219 ± 0.013
0.388CysIle: 0.388 ± 0.019
0.219CysLys: 0.219 ± 0.015
0.759CysLeu: 0.759 ± 0.027
0.164CysMet: 0.164 ± 0.012
0.221CysAsn: 0.221 ± 0.013
0.402CysPro: 0.402 ± 0.021
0.242CysGln: 0.242 ± 0.015
0.464CysArg: 0.464 ± 0.022
0.475CysSer: 0.475 ± 0.02
0.443CysThr: 0.443 ± 0.021
0.598CysVal: 0.598 ± 0.024
0.1CysTrp: 0.1 ± 0.009
0.18CysTyr: 0.18 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.033AspAla: 7.033 ± 0.107
0.451AspCys: 0.451 ± 0.02
3.68AspAsp: 3.68 ± 0.092
4.275AspGlu: 4.275 ± 0.064
2.263AspPhe: 2.263 ± 0.045
5.553AspGly: 5.553 ± 0.135
1.416AspHis: 1.416 ± 0.037
3.418AspIle: 3.418 ± 0.056
2.137AspLys: 2.137 ± 0.045
6.476AspLeu: 6.476 ± 0.092
1.674AspMet: 1.674 ± 0.043
1.499AspAsn: 1.499 ± 0.035
3.375AspPro: 3.375 ± 0.058
2.113AspGln: 2.113 ± 0.044
3.88AspArg: 3.88 ± 0.063
2.214AspSer: 2.214 ± 0.046
3.143AspThr: 3.143 ± 0.091
4.919AspVal: 4.919 ± 0.083
0.999AspTrp: 0.999 ± 0.023
1.614AspTyr: 1.614 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
7.565GluAla: 7.565 ± 0.095
0.385GluCys: 0.385 ± 0.017
3.549GluAsp: 3.549 ± 0.056
4.552GluGlu: 4.552 ± 0.096
2.063GluPhe: 2.063 ± 0.047
5.157GluGly: 5.157 ± 0.082
1.275GluHis: 1.275 ± 0.038
3.848GluIle: 3.848 ± 0.076
2.952GluLys: 2.952 ± 0.065
5.921GluLeu: 5.921 ± 0.08
1.852GluMet: 1.852 ± 0.043
2.149GluAsn: 2.149 ± 0.046
2.808GluPro: 2.808 ± 0.062
2.347GluGln: 2.347 ± 0.045
4.649GluArg: 4.649 ± 0.074
2.621GluSer: 2.621 ± 0.055
4.428GluThr: 4.428 ± 0.078
4.258GluVal: 4.258 ± 0.069
0.732GluTrp: 0.732 ± 0.026
1.137GluTyr: 1.137 ± 0.036
0.0GluXaa: 0.0 ± 0.0
Phe
4.345PheAla: 4.345 ± 0.064
0.43PheCys: 0.43 ± 0.022
2.946PheAsp: 2.946 ± 0.055
2.49PheGlu: 2.49 ± 0.052
1.707PhePhe: 1.707 ± 0.043
3.724PheGly: 3.724 ± 0.073
0.78PheHis: 0.78 ± 0.025
1.749PheIle: 1.749 ± 0.041
1.166PheLys: 1.166 ± 0.033
3.76PheLeu: 3.76 ± 0.077
0.893PheMet: 0.893 ± 0.029
1.162PheAsn: 1.162 ± 0.029
1.516PhePro: 1.516 ± 0.045
1.097PheGln: 1.097 ± 0.032
1.978PheArg: 1.978 ± 0.045
2.543PheSer: 2.543 ± 0.054
2.142PheThr: 2.142 ± 0.049
3.013PheVal: 3.013 ± 0.058
0.604PheTrp: 0.604 ± 0.022
1.034PheTyr: 1.034 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
8.766GlyAla: 8.766 ± 0.133
0.718GlyCys: 0.718 ± 0.025
5.178GlyAsp: 5.178 ± 0.177
5.06GlyGlu: 5.06 ± 0.065
3.708GlyPhe: 3.708 ± 0.063
7.406GlyGly: 7.406 ± 0.193
1.855GlyHis: 1.855 ± 0.046
4.639GlyIle: 4.639 ± 0.063
3.231GlyLys: 3.231 ± 0.059
8.689GlyLeu: 8.689 ± 0.101
2.264GlyMet: 2.264 ± 0.045
2.564GlyAsn: 2.564 ± 0.131
3.427GlyPro: 3.427 ± 0.05
2.683GlyGln: 2.683 ± 0.055
4.981GlyArg: 4.981 ± 0.068
4.897GlySer: 4.897 ± 0.095
4.97GlyThr: 4.97 ± 0.096
6.303GlyVal: 6.303 ± 0.077
1.303GlyTrp: 1.303 ± 0.041
2.256GlyTyr: 2.256 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
2.04HisAla: 2.04 ± 0.044
0.236HisCys: 0.236 ± 0.014
1.104HisAsp: 1.104 ± 0.036
1.22HisGlu: 1.22 ± 0.035
0.95HisPhe: 0.95 ± 0.029
1.787HisGly: 1.787 ± 0.049
0.562HisHis: 0.562 ± 0.027
1.059HisIle: 1.059 ± 0.031
0.692HisLys: 0.692 ± 0.027
2.192HisLeu: 2.192 ± 0.055
0.54HisMet: 0.54 ± 0.025
0.543HisAsn: 0.543 ± 0.021
1.276HisPro: 1.276 ± 0.035
0.678HisGln: 0.678 ± 0.024
1.162HisArg: 1.162 ± 0.034
1.071HisSer: 1.071 ± 0.034
0.938HisThr: 0.938 ± 0.029
1.525HisVal: 1.525 ± 0.033
0.315HisTrp: 0.315 ± 0.017
0.634HisTyr: 0.634 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.875IleAla: 6.875 ± 0.081
0.494IleCys: 0.494 ± 0.021
3.805IleAsp: 3.805 ± 0.063
3.938IleGlu: 3.938 ± 0.06
1.838IlePhe: 1.838 ± 0.041
4.817IleGly: 4.817 ± 0.068
0.942IleHis: 0.942 ± 0.032
2.493IleIle: 2.493 ± 0.049
1.674IleLys: 1.674 ± 0.038
4.351IleLeu: 4.351 ± 0.067
1.092IleMet: 1.092 ± 0.027
1.733IleAsn: 1.733 ± 0.045
2.315IlePro: 2.315 ± 0.045
1.334IleGln: 1.334 ± 0.034
2.789IleArg: 2.789 ± 0.054
3.392IleSer: 3.392 ± 0.062
2.986IleThr: 2.986 ± 0.055
4.211IleVal: 4.211 ± 0.064
0.687IleTrp: 0.687 ± 0.023
1.229IleTyr: 1.229 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
4.195LysAla: 4.195 ± 0.089
0.203LysCys: 0.203 ± 0.012
2.138LysAsp: 2.138 ± 0.045
2.333LysGlu: 2.333 ± 0.053
1.096LysPhe: 1.096 ± 0.032
2.812LysGly: 2.812 ± 0.062
0.708LysHis: 0.708 ± 0.025
2.037LysIle: 2.037 ± 0.043
1.898LysLys: 1.898 ± 0.083
3.498LysLeu: 3.498 ± 0.056
0.917LysMet: 0.917 ± 0.03
1.036LysAsn: 1.036 ± 0.033
2.041LysPro: 2.041 ± 0.045
1.139LysGln: 1.139 ± 0.033
2.512LysArg: 2.512 ± 0.051
2.211LysSer: 2.211 ± 0.048
2.327LysThr: 2.327 ± 0.05
2.566LysVal: 2.566 ± 0.049
0.472LysTrp: 0.472 ± 0.021
0.756LysTyr: 0.756 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
11.347LeuAla: 11.347 ± 0.108
0.76LeuCys: 0.76 ± 0.027
6.007LeuAsp: 6.007 ± 0.074
6.047LeuGlu: 6.047 ± 0.081
3.827LeuPhe: 3.827 ± 0.085
8.268LeuGly: 8.268 ± 0.1
1.813LeuHis: 1.813 ± 0.044
5.324LeuIle: 5.324 ± 0.077
3.97LeuLys: 3.97 ± 0.076
8.876LeuLeu: 8.876 ± 0.134
2.485LeuMet: 2.485 ± 0.05
3.029LeuAsn: 3.029 ± 0.05
5.004LeuPro: 5.004 ± 0.085
2.845LeuGln: 2.845 ± 0.054
5.408LeuArg: 5.408 ± 0.069
6.989LeuSer: 6.989 ± 0.078
6.139LeuThr: 6.139 ± 0.079
7.112LeuVal: 7.112 ± 0.095
1.131LeuTrp: 1.131 ± 0.034
2.082LeuTyr: 2.082 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
2.921MetAla: 2.921 ± 0.052
0.161MetCys: 0.161 ± 0.011
1.353MetAsp: 1.353 ± 0.033
1.394MetGlu: 1.394 ± 0.036
0.824MetPhe: 0.824 ± 0.026
2.184MetGly: 2.184 ± 0.051
0.452MetHis: 0.452 ± 0.021
1.329MetIle: 1.329 ± 0.037
1.102MetLys: 1.102 ± 0.033
2.253MetLeu: 2.253 ± 0.046
0.739MetMet: 0.739 ± 0.025
0.862MetAsn: 0.862 ± 0.029
1.426MetPro: 1.426 ± 0.044
0.812MetGln: 0.812 ± 0.03
1.548MetArg: 1.548 ± 0.036
2.033MetSer: 2.033 ± 0.042
1.828MetThr: 1.828 ± 0.044
1.781MetVal: 1.781 ± 0.04
0.261MetTrp: 0.261 ± 0.016
0.363MetTyr: 0.363 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.322AsnAla: 3.322 ± 0.065
0.252AsnCys: 0.252 ± 0.014
1.686AsnAsp: 1.686 ± 0.06
1.737AsnGlu: 1.737 ± 0.042
1.119AsnPhe: 1.119 ± 0.031
2.777AsnGly: 2.777 ± 0.123
0.603AsnHis: 0.603 ± 0.023
1.616AsnIle: 1.616 ± 0.039
0.982AsnLys: 0.982 ± 0.03
2.903AsnLeu: 2.903 ± 0.051
0.76AsnMet: 0.76 ± 0.025
0.88AsnAsn: 0.88 ± 0.04
1.861AsnPro: 1.861 ± 0.04
0.957AsnGln: 0.957 ± 0.029
1.77AsnArg: 1.77 ± 0.041
1.469AsnSer: 1.469 ± 0.042
1.523AsnThr: 1.523 ± 0.045
2.276AsnVal: 2.276 ± 0.046
0.48AsnTrp: 0.48 ± 0.021
0.719AsnTyr: 0.719 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
4.939ProAla: 4.939 ± 0.075
0.329ProCys: 0.329 ± 0.017
3.598ProAsp: 3.598 ± 0.054
3.837ProGlu: 3.837 ± 0.062
2.002ProPhe: 2.002 ± 0.046
3.913ProGly: 3.913 ± 0.069
1.038ProHis: 1.038 ± 0.027
2.443ProIle: 2.443 ± 0.053
1.871ProLys: 1.871 ± 0.044
4.541ProLeu: 4.541 ± 0.075
1.107ProMet: 1.107 ± 0.033
1.496ProAsn: 1.496 ± 0.04
1.965ProPro: 1.965 ± 0.049
1.413ProGln: 1.413 ± 0.037
2.225ProArg: 2.225 ± 0.047
2.88ProSer: 2.88 ± 0.053
2.725ProThr: 2.725 ± 0.055
3.799ProVal: 3.799 ± 0.062
0.623ProTrp: 0.623 ± 0.025
1.186ProTyr: 1.186 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
3.718GlnAla: 3.718 ± 0.064
0.205GlnCys: 0.205 ± 0.014
1.566GlnAsp: 1.566 ± 0.036
1.902GlnGlu: 1.902 ± 0.04
1.181GlnPhe: 1.181 ± 0.029
2.345GlnGly: 2.345 ± 0.049
0.588GlnHis: 0.588 ± 0.024
1.867GlnIle: 1.867 ± 0.041
1.339GlnLys: 1.339 ± 0.032
2.926GlnLeu: 2.926 ± 0.061
0.941GlnMet: 0.941 ± 0.031
1.003GlnAsn: 1.003 ± 0.027
1.45GlnPro: 1.45 ± 0.038
1.1GlnGln: 1.1 ± 0.03
1.866GlnArg: 1.866 ± 0.041
2.016GlnSer: 2.016 ± 0.04
1.917GlnThr: 1.917 ± 0.039
2.429GlnVal: 2.429 ± 0.048
0.357GlnTrp: 0.357 ± 0.019
0.656GlnTyr: 0.656 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
6.427ArgAla: 6.427 ± 0.088
0.418ArgCys: 0.418 ± 0.019
3.617ArgAsp: 3.617 ± 0.061
3.771ArgGlu: 3.771 ± 0.066
2.628ArgPhe: 2.628 ± 0.051
3.89ArgGly: 3.89 ± 0.059
1.37ArgHis: 1.37 ± 0.037
3.173ArgIle: 3.173 ± 0.055
2.091ArgLys: 2.091 ± 0.05
6.512ArgLeu: 6.512 ± 0.081
1.554ArgMet: 1.554 ± 0.039
1.733ArgAsn: 1.733 ± 0.04
2.709ArgPro: 2.709 ± 0.051
2.032ArgGln: 2.032 ± 0.05
4.046ArgArg: 4.046 ± 0.073
3.37ArgSer: 3.37 ± 0.059
3.206ArgThr: 3.206 ± 0.053
4.226ArgVal: 4.226 ± 0.06
0.8ArgTrp: 0.8 ± 0.024
1.527ArgTyr: 1.527 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
6.25SerAla: 6.25 ± 0.083
0.434SerCys: 0.434 ± 0.019
3.842SerAsp: 3.842 ± 0.073
3.619SerGlu: 3.619 ± 0.058
2.565SerPhe: 2.565 ± 0.059
5.496SerGly: 5.496 ± 0.092
1.193SerHis: 1.193 ± 0.038
3.179SerIle: 3.179 ± 0.06
2.033SerLys: 2.033 ± 0.045
6.002SerLeu: 6.002 ± 0.08
1.517SerMet: 1.517 ± 0.037
1.744SerAsn: 1.744 ± 0.043
2.686SerPro: 2.686 ± 0.051
1.724SerGln: 1.724 ± 0.036
3.08SerArg: 3.08 ± 0.059
3.523SerSer: 3.523 ± 0.07
3.026SerThr: 3.026 ± 0.062
4.531SerVal: 4.531 ± 0.074
0.763SerTrp: 0.763 ± 0.025
1.389SerTyr: 1.389 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
6.091ThrAla: 6.091 ± 0.092
0.475ThrCys: 0.475 ± 0.019
3.489ThrAsp: 3.489 ± 0.076
3.198ThrGlu: 3.198 ± 0.057
2.293ThrPhe: 2.293 ± 0.048
5.314ThrGly: 5.314 ± 0.099
1.248ThrHis: 1.248 ± 0.035
3.202ThrIle: 3.202 ± 0.059
1.841ThrLys: 1.841 ± 0.048
6.019ThrLeu: 6.019 ± 0.082
1.304ThrMet: 1.304 ± 0.035
1.669ThrAsn: 1.669 ± 0.042
3.356ThrPro: 3.356 ± 0.068
1.686ThrGln: 1.686 ± 0.037
3.149ThrArg: 3.149 ± 0.051
3.524ThrSer: 3.524 ± 0.06
3.113ThrThr: 3.113 ± 0.078
4.231ThrVal: 4.231 ± 0.082
0.744ThrTrp: 0.744 ± 0.024
1.537ThrTyr: 1.537 ± 0.046
0.0ThrXaa: 0.0 ± 0.0
Val
8.367ValAla: 8.367 ± 0.102
0.639ValCys: 0.639 ± 0.022
4.578ValAsp: 4.578 ± 0.092
4.773ValGlu: 4.773 ± 0.069
2.76ValPhe: 2.76 ± 0.051
6.054ValGly: 6.054 ± 0.095
1.417ValHis: 1.417 ± 0.037
3.864ValIle: 3.864 ± 0.061
2.332ValLys: 2.332 ± 0.045
7.061ValLeu: 7.061 ± 0.097
1.855ValMet: 1.855 ± 0.044
2.178ValAsn: 2.178 ± 0.05
3.649ValPro: 3.649 ± 0.054
2.14ValGln: 2.14 ± 0.046
4.4ValArg: 4.4 ± 0.065
4.902ValSer: 4.902 ± 0.081
4.669ValThr: 4.669 ± 0.082
5.39ValVal: 5.39 ± 0.075
0.884ValTrp: 0.884 ± 0.027
1.517ValTyr: 1.517 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.258TrpAla: 1.258 ± 0.034
0.137TrpCys: 0.137 ± 0.011
0.692TrpAsp: 0.692 ± 0.026
0.658TrpGlu: 0.658 ± 0.025
0.561TrpPhe: 0.561 ± 0.023
0.996TrpGly: 0.996 ± 0.031
0.328TrpHis: 0.328 ± 0.017
0.694TrpIle: 0.694 ± 0.027
0.485TrpLys: 0.485 ± 0.022
1.462TrpLeu: 1.462 ± 0.039
0.411TrpMet: 0.411 ± 0.019
0.409TrpAsn: 0.409 ± 0.02
0.613TrpPro: 0.613 ± 0.025
0.568TrpGln: 0.568 ± 0.022
0.911TrpArg: 0.911 ± 0.026
0.843TrpSer: 0.843 ± 0.029
0.754TrpThr: 0.754 ± 0.023
0.932TrpVal: 0.932 ± 0.032
0.216TrpTrp: 0.216 ± 0.014
0.307TrpTyr: 0.307 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.452TyrAla: 2.452 ± 0.051
0.253TyrCys: 0.253 ± 0.014
1.644TyrAsp: 1.644 ± 0.039
1.475TyrGlu: 1.475 ± 0.041
1.045TyrPhe: 1.045 ± 0.027
2.104TyrGly: 2.104 ± 0.052
0.498TyrHis: 0.498 ± 0.021
0.96TyrIle: 0.96 ± 0.032
0.761TyrLys: 0.761 ± 0.024
2.425TyrLeu: 2.425 ± 0.045
0.521TyrMet: 0.521 ± 0.02
0.619TyrAsn: 0.619 ± 0.025
1.008TyrPro: 1.008 ± 0.032
0.851TyrGln: 0.851 ± 0.027
1.579TyrArg: 1.579 ± 0.039
1.298TyrSer: 1.298 ± 0.039
1.147TyrThr: 1.147 ± 0.043
1.716TyrVal: 1.716 ± 0.04
0.408TyrTrp: 0.408 ± 0.02
0.602TyrTyr: 0.602 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3729 proteins (1163080 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski