Capybara microvirus Cap1_SP_77
Average proteome isoelectric point is 5.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVN1|A0A4V1FVN1_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_77 OX=2584791 PE=4 SV=1
MM1 pKa = 7.46 SNVFIEE7 pKa = 4.7 RR8 pKa = 11.84 EE9 pKa = 4.01 NFSPNIKK16 pKa = 10.31 RR17 pKa = 11.84 NVFDD21 pKa = 5.95 LSHH24 pKa = 6.29 QNNLSLKK31 pKa = 9.12 IGKK34 pKa = 8.0 ITPIYY39 pKa = 8.86 CQEE42 pKa = 4.08 VLPGTSVKK50 pKa = 9.85 MDD52 pKa = 3.24 VVSALRR58 pKa = 11.84 AAPLFFPIQTKK69 pKa = 10.17 IKK71 pKa = 9.69 ASMSFFYY78 pKa = 10.89 VRR80 pKa = 11.84 NRR82 pKa = 11.84 NLWDD86 pKa = 3.62 DD87 pKa = 3.31 WTKK90 pKa = 10.78 FISNEE95 pKa = 4.17 EE96 pKa = 4.12 TSSTFPVIDD105 pKa = 3.62 ASKK108 pKa = 10.12 NPEE111 pKa = 3.83 FFKK114 pKa = 11.24 VGGLADD120 pKa = 3.86 YY121 pKa = 10.93 LGVPTAFYY129 pKa = 10.16 GYY131 pKa = 7.09 PTASLTTALDD141 pKa = 3.65 AEE143 pKa = 4.6 EE144 pKa = 4.16 VLSNLEE150 pKa = 3.5 TSYY153 pKa = 11.51 YY154 pKa = 9.46 EE155 pKa = 4.29 KK156 pKa = 10.73 KK157 pKa = 10.62 KK158 pKa = 9.27 STHH161 pKa = 5.21 SLAYY165 pKa = 8.99 VQHH168 pKa = 6.71 SYY170 pKa = 11.25 DD171 pKa = 3.44 VSSDD175 pKa = 3.24 RR176 pKa = 11.84 MAGYY180 pKa = 10.3 CGIINSTITPGVKK193 pKa = 9.99 FSAVSFGGNRR203 pKa = 11.84 SGEE206 pKa = 4.44 SVVGNYY212 pKa = 9.16 DD213 pKa = 3.85 LPWTAGGFTYY223 pKa = 10.79 NKK225 pKa = 8.29 YY226 pKa = 9.48 GRR228 pKa = 11.84 VYY230 pKa = 10.67 RR231 pKa = 11.84 VTKK234 pKa = 10.45 KK235 pKa = 10.32 LASGNMTVKK244 pKa = 10.38 SRR246 pKa = 11.84 SACSLMVIVNGTVVAVQTGSYY267 pKa = 10.37 ASSTTTIGLLQTVVDD282 pKa = 5.84 AINKK286 pKa = 10.03 AIDD289 pKa = 4.09 DD290 pKa = 4.15 GQVVHH295 pKa = 6.75 FCVFTQGTQTSFSPSTIYY313 pKa = 10.79 YY314 pKa = 9.29 IEE316 pKa = 3.98 QLIVQYY322 pKa = 11.31 DD323 pKa = 3.7 NTDD326 pKa = 3.11 ATEE329 pKa = 3.82 WKK331 pKa = 10.23 AISGYY336 pKa = 10.56 NSYY339 pKa = 11.45 NPFLADD345 pKa = 3.61 GMKK348 pKa = 10.57 LSALPFRR355 pKa = 11.84 AYY357 pKa = 10.18 EE358 pKa = 3.99 SVYY361 pKa = 9.5 NCFYY365 pKa = 10.45 RR366 pKa = 11.84 KK367 pKa = 9.51 EE368 pKa = 4.12 RR369 pKa = 11.84 VDD371 pKa = 3.54 PNRR374 pKa = 11.84 VNGKK378 pKa = 9.11 IEE380 pKa = 3.97 YY381 pKa = 9.94 DD382 pKa = 3.91 VYY384 pKa = 11.12 CDD386 pKa = 3.76 KK387 pKa = 11.58 SGGNDD392 pKa = 2.82 TNYY395 pKa = 10.22 YY396 pKa = 9.93 HH397 pKa = 7.48 LYY399 pKa = 9.2 DD400 pKa = 4.93 APWEE404 pKa = 4.15 TDD406 pKa = 2.84 QFTSCLPTPQAGVAPLVGLTSNFSGVVSATFLDD439 pKa = 4.12 EE440 pKa = 4.33 SGNTVKK446 pKa = 10.85 YY447 pKa = 10.32 DD448 pKa = 3.59 VQSVTDD454 pKa = 3.7 SSGKK458 pKa = 8.01 ITGFAYY464 pKa = 10.58 SEE466 pKa = 4.85 DD467 pKa = 3.95 IPWSVRR473 pKa = 11.84 EE474 pKa = 3.96 QLNHH478 pKa = 6.04 SVLSGFDD485 pKa = 3.28 INSLRR490 pKa = 11.84 NVSAFQIYY498 pKa = 10.45 LEE500 pKa = 4.29 NKK502 pKa = 8.66 LRR504 pKa = 11.84 RR505 pKa = 11.84 GLKK508 pKa = 10.09 YY509 pKa = 9.42 VDD511 pKa = 3.64 QIKK514 pKa = 10.15 SHH516 pKa = 6.78 FGVQPSYY523 pKa = 10.89 EE524 pKa = 4.09 ALNMPEE530 pKa = 4.11 YY531 pKa = 10.32 IGGVTEE537 pKa = 4.27 TFNVNQISSTIEE549 pKa = 3.57 NDD551 pKa = 3.33 KK552 pKa = 11.23 VSLGDD557 pKa = 3.33 YY558 pKa = 9.63 AGQMTCMAHH567 pKa = 6.24 PNHH570 pKa = 6.67 NISHH574 pKa = 6.14 YY575 pKa = 9.83 CDD577 pKa = 2.98 EE578 pKa = 4.89 HH579 pKa = 7.76 GFIIGVLSIVPIPNYY594 pKa = 10.41 SQALPKK600 pKa = 10.59 FFGKK604 pKa = 7.32 TTTFDD609 pKa = 3.83 YY610 pKa = 8.18 YY611 pKa = 10.93 TPEE614 pKa = 4.25 LDD616 pKa = 4.3 KK617 pKa = 11.56 LSLQPVRR624 pKa = 11.84 NWEE627 pKa = 3.88 IAPVQTFANADD638 pKa = 3.52 SGSKK642 pKa = 9.72 IEE644 pKa = 4.6 ALDD647 pKa = 3.76 GVFGYY652 pKa = 9.28 QLPWYY657 pKa = 8.36 EE658 pKa = 4.01 YY659 pKa = 10.5 KK660 pKa = 10.9 GALDD664 pKa = 3.81 TVHH667 pKa = 8.04 GEE669 pKa = 3.68 MRR671 pKa = 11.84 TDD673 pKa = 3.76 LRR675 pKa = 11.84 DD676 pKa = 3.52 FMMNRR681 pKa = 11.84 VFRR684 pKa = 11.84 TTPQLGSDD692 pKa = 3.8 FVYY695 pKa = 11.14 VDD697 pKa = 3.75 GEE699 pKa = 4.55 SVNNVFNVQDD709 pKa = 4.14 DD710 pKa = 4.51 DD711 pKa = 3.89 VDD713 pKa = 4.14 KK714 pKa = 10.88 IYY716 pKa = 10.73 GQIYY720 pKa = 9.59 FDD722 pKa = 4.28 VKK724 pKa = 9.6 TKK726 pKa = 10.88 HH727 pKa = 5.21 PMSRR731 pKa = 11.84 IGQPVYY737 pKa = 10.36 HH738 pKa = 7.06
Molecular weight: 82.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.086
IPC2_protein 5.232
IPC_protein 5.232
Toseland 5.334
ProMoST 5.397
Dawson 5.321
Bjellqvist 5.41
Wikipedia 5.245
Rodwell 5.245
Grimsley 5.308
Solomon 5.321
Lehninger 5.296
Nozaki 5.486
DTASelect 5.677
Thurlkill 5.397
EMBOSS 5.385
Sillero 5.563
Patrickios 3.198
IPC_peptide 5.334
IPC2_peptide 5.563
IPC2.peptide.svr19 5.477
Protein with the highest isoelectric point:
>tr|A0A4P8W4R6|A0A4P8W4R6_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_77 OX=2584791 PE=3 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.17 CLNPVIMINRR12 pKa = 11.84 PFLYY16 pKa = 10.54 SFDD19 pKa = 3.58 GRR21 pKa = 11.84 IEE23 pKa = 3.84 FANIDD28 pKa = 3.76 NNIISHH34 pKa = 6.76 PSPYY38 pKa = 10.31 DD39 pKa = 3.21 VEE41 pKa = 4.51 FAANVALCSLPKK53 pKa = 10.46 NEE55 pKa = 4.74 EE56 pKa = 3.95 GNVARR61 pKa = 11.84 FDD63 pKa = 4.07 YY64 pKa = 10.56 SVHH67 pKa = 6.0 PFEE70 pKa = 5.32 IAVSGYY76 pKa = 9.96 VDD78 pKa = 3.63 GVFYY82 pKa = 10.02 PASNPCMKK90 pKa = 10.16 VFLLQVPCRR99 pKa = 11.84 KK100 pKa = 9.87 CEE102 pKa = 3.97 LCCEE106 pKa = 4.18 NRR108 pKa = 11.84 KK109 pKa = 6.64 QTYY112 pKa = 5.68 VHH114 pKa = 7.2 RR115 pKa = 11.84 IMFEE119 pKa = 4.11 AEE121 pKa = 3.91 SSRR124 pKa = 11.84 QVDD127 pKa = 4.34 FVTLTFDD134 pKa = 3.58 RR135 pKa = 11.84 DD136 pKa = 3.72 HH137 pKa = 6.59 YY138 pKa = 10.01 PSRR141 pKa = 11.84 GATKK145 pKa = 10.35 QEE147 pKa = 3.75 LQRR150 pKa = 11.84 FIDD153 pKa = 3.67 SFRR156 pKa = 11.84 KK157 pKa = 9.19 FIKK160 pKa = 9.92 RR161 pKa = 11.84 YY162 pKa = 8.31 SEE164 pKa = 3.8 EE165 pKa = 3.93 RR166 pKa = 11.84 PRR168 pKa = 11.84 TPKK171 pKa = 10.43 VFDD174 pKa = 3.7 GDD176 pKa = 3.25 KK177 pKa = 10.15 FRR179 pKa = 11.84 YY180 pKa = 9.51 IFVSEE185 pKa = 4.25 YY186 pKa = 7.91 GHH188 pKa = 6.92 KK189 pKa = 10.81 GRR191 pKa = 11.84 GHH193 pKa = 5.1 YY194 pKa = 9.78 HH195 pKa = 6.96 GEE197 pKa = 4.11 MFNLPPYY204 pKa = 10.65 EE205 pKa = 4.17 EE206 pKa = 4.32 FKK208 pKa = 10.91 EE209 pKa = 4.66 LYY211 pKa = 9.55 ILQHH215 pKa = 5.56 GTTLKK220 pKa = 11.08 YY221 pKa = 9.62 ITDD224 pKa = 3.97 PQNSRR229 pKa = 11.84 TVFYY233 pKa = 10.75 EE234 pKa = 4.01 DD235 pKa = 4.73 LNPAPFYY242 pKa = 9.99 KK243 pKa = 10.37 LKK245 pKa = 10.88 RR246 pKa = 11.84 KK247 pKa = 8.83 GCPVLEE253 pKa = 4.37 PSVHH257 pKa = 7.04 RR258 pKa = 11.84 DD259 pKa = 3.39 YY260 pKa = 11.47 LYY262 pKa = 10.92 LDD264 pKa = 3.54 YY265 pKa = 10.92 YY266 pKa = 9.49 VRR268 pKa = 11.84 KK269 pKa = 9.32 IWNNGLTCSEE279 pKa = 4.24 PTKK282 pKa = 10.89 DD283 pKa = 4.25 SAGSYY288 pKa = 7.9 CAKK291 pKa = 10.44 YY292 pKa = 9.89 INKK295 pKa = 8.71 PSEE298 pKa = 4.15 MVPEE302 pKa = 4.94 GCDD305 pKa = 2.92 KK306 pKa = 11.29 CFVLASNKK314 pKa = 10.17 NGGIGSQYY322 pKa = 9.31 CKK324 pKa = 9.88 TVIRR328 pKa = 11.84 EE329 pKa = 4.04 FLKK332 pKa = 10.92 KK333 pKa = 10.53 NPTALVYY340 pKa = 10.43 KK341 pKa = 9.39 FRR343 pKa = 11.84 PRR345 pKa = 11.84 HH346 pKa = 5.32 SSLIYY351 pKa = 9.03 NIPIIGYY358 pKa = 8.4 VKK360 pKa = 10.57 NLALPRR366 pKa = 11.84 PSDD369 pKa = 3.7 VLSQRR374 pKa = 11.84 YY375 pKa = 8.72 VDD377 pKa = 3.71 SFKK380 pKa = 10.54 IYY382 pKa = 9.09 HH383 pKa = 6.83 WYY385 pKa = 10.38 NRR387 pKa = 11.84 MIGDD391 pKa = 3.45 VLEE394 pKa = 4.24 YY395 pKa = 10.65 QFAEE399 pKa = 4.15 AFFAPRR405 pKa = 11.84 GCIKK409 pKa = 10.33 PFRR412 pKa = 11.84 STDD415 pKa = 3.37 YY416 pKa = 10.34 RR417 pKa = 11.84 RR418 pKa = 11.84 KK419 pKa = 10.55 NPTEE423 pKa = 3.64 YY424 pKa = 10.94 EE425 pKa = 3.95 KK426 pKa = 10.75 FIKK429 pKa = 10.22 KK430 pKa = 9.36 NRR432 pKa = 11.84 SKK434 pKa = 11.5 EE435 pKa = 4.11 EE436 pKa = 3.33 ISKK439 pKa = 10.47 IEE441 pKa = 4.28 DD442 pKa = 3.18 YY443 pKa = 11.2 LYY445 pKa = 10.31 IEE447 pKa = 4.78 RR448 pKa = 11.84 QNIDD452 pKa = 2.62 WNEE455 pKa = 3.79 IFKK458 pKa = 10.47 LAHH461 pKa = 6.04 LRR463 pKa = 11.84 VINNKK468 pKa = 9.04 AFRR471 pKa = 11.84 DD472 pKa = 3.88 SEE474 pKa = 3.98 IPYY477 pKa = 9.91 IDD479 pKa = 4.76 DD480 pKa = 4.31 LSDD483 pKa = 3.14 EE484 pKa = 4.14 LRR486 pKa = 11.84 EE487 pKa = 4.05 VRR489 pKa = 11.84 SKK491 pKa = 11.26 NLWRR495 pKa = 11.84 KK496 pKa = 8.51 EE497 pKa = 4.0 RR498 pKa = 11.84 IKK500 pKa = 11.06 LL501 pKa = 3.62
Molecular weight: 59.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.209
IPC2_protein 8.258
IPC_protein 8.097
Toseland 8.2
ProMoST 8.624
Dawson 8.887
Bjellqvist 9.004
Wikipedia 8.96
Rodwell 8.96
Grimsley 8.375
Solomon 8.946
Lehninger 8.916
Nozaki 9.092
DTASelect 8.829
Thurlkill 8.873
EMBOSS 9.033
Sillero 9.121
Patrickios 4.151
IPC_peptide 8.931
IPC2_peptide 7.849
IPC2.peptide.svr19 7.88
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1777
72
738
355.4
40.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.684 ± 1.18
1.463 ± 0.538
5.796 ± 0.476
6.134 ± 1.03
5.008 ± 0.858
5.459 ± 0.77
1.745 ± 0.324
6.19 ± 0.754
5.965 ± 0.785
7.485 ± 0.879
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.364 ± 0.597
6.922 ± 1.012
4.389 ± 0.721
4.615 ± 1.435
5.29 ± 1.091
7.372 ± 0.915
5.402 ± 0.996
6.415 ± 1.394
1.182 ± 0.193
5.121 ± 1.128
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here