Mycobacterium phage Herbertwm

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TGV1|A0A5J6TGV1_9CAUD Uncharacterized protein OS=Mycobacterium phage Herbertwm OX=2601640 GN=84 PE=4 SV=1
MM1 pKa = 7.79PLYY4 pKa = 9.48KK5 pKa = 8.52TTLTIEE11 pKa = 4.08NFEE14 pKa = 4.96DD15 pKa = 3.74LDD17 pKa = 4.25QIADD21 pKa = 3.56AHH23 pKa = 5.78ATGAFTNRR31 pKa = 11.84DD32 pKa = 3.44RR33 pKa = 11.84YY34 pKa = 9.79EE35 pKa = 4.14GHH37 pKa = 7.6PEE39 pKa = 3.66MQNNCNVVWAAKK51 pKa = 10.2ALQTYY56 pKa = 8.97VNVVGRR62 pKa = 11.84DD63 pKa = 3.6TIEE66 pKa = 4.04TSVSDD71 pKa = 3.76LLGDD75 pKa = 5.05LMHH78 pKa = 7.03LCDD81 pKa = 5.33AAGLDD86 pKa = 4.37FDD88 pKa = 4.7EE89 pKa = 5.24LVNKK93 pKa = 10.64AEE95 pKa = 3.98YY96 pKa = 10.17DD97 pKa = 3.58YY98 pKa = 11.49GAEE101 pKa = 4.46VEE103 pKa = 4.66DD104 pKa = 4.25LQQ106 pKa = 5.83

Molecular weight: 11.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TBX2|A0A5J6TBX2_9CAUD Uncharacterized protein OS=Mycobacterium phage Herbertwm OX=2601640 GN=87 PE=4 SV=1
MM1 pKa = 7.34TATLTRR7 pKa = 11.84PATMTQAMAHH17 pKa = 6.02QITVGLIRR25 pKa = 11.84EE26 pKa = 4.32HH27 pKa = 7.14GLVGWKK33 pKa = 10.22VKK35 pKa = 10.46LDD37 pKa = 3.49NARR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84AGLCDD47 pKa = 3.37HH48 pKa = 6.24TNKK51 pKa = 10.11VISLSKK57 pKa = 10.16HH58 pKa = 4.93LMAIRR63 pKa = 11.84SYY65 pKa = 9.95EE66 pKa = 4.0DD67 pKa = 2.82TMMTITHH74 pKa = 6.78EE75 pKa = 4.06LAHH78 pKa = 6.47ALVGPRR84 pKa = 11.84HH85 pKa = 5.46GHH87 pKa = 5.7DD88 pKa = 3.56AVWAAKK94 pKa = 9.58HH95 pKa = 5.99RR96 pKa = 11.84SLGGNGKK103 pKa = 9.46RR104 pKa = 11.84CFQLEE109 pKa = 4.37EE110 pKa = 4.12VDD112 pKa = 5.1RR113 pKa = 11.84TAPWLGTCDD122 pKa = 3.37HH123 pKa = 6.98GKK125 pKa = 9.53QFARR129 pKa = 11.84YY130 pKa = 7.65RR131 pKa = 11.84QPKK134 pKa = 9.6RR135 pKa = 11.84LDD137 pKa = 2.95GWRR140 pKa = 11.84CRR142 pKa = 11.84CTAGASPVVWKK153 pKa = 10.66RR154 pKa = 11.84NRR156 pKa = 3.17

Molecular weight: 17.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

16198

22

807

184.1

20.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.581 ± 0.355

1.031 ± 0.13

6.667 ± 0.174

6.402 ± 0.354

3.426 ± 0.184

8.155 ± 0.492

2.13 ± 0.171

5.106 ± 0.178

4.741 ± 0.26

8.199 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.587 ± 0.109

3.272 ± 0.218

5.476 ± 0.235

3.747 ± 0.243

6.303 ± 0.326

5.34 ± 0.237

5.871 ± 0.224

6.908 ± 0.188

2.068 ± 0.137

2.988 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski