Nitratiruptor sp. (strain SB155-2)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Nautiliales; Nitratiruptoraceae; Nitratiruptor; unclassified Nitratiruptor

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1843 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6Q4W2|A6Q4W2_NITSB Polysaccharide biosynthesis protein OS=Nitratiruptor sp. (strain SB155-2) OX=387092 GN=NIS_1414 PE=4 SV=1
MM1 pKa = 7.48LTRR4 pKa = 11.84DD5 pKa = 4.29DD6 pKa = 3.91VAGLYY11 pKa = 9.62IALFQRR17 pKa = 11.84APSKK21 pKa = 11.16SEE23 pKa = 3.66LDD25 pKa = 2.82TWYY28 pKa = 11.25NDD30 pKa = 3.06VVANNRR36 pKa = 11.84DD37 pKa = 3.49LADD40 pKa = 3.5TAEE43 pKa = 4.3TMLQAAQLAVNGFGLQDD60 pKa = 5.12LYY62 pKa = 10.71PQYY65 pKa = 11.89ANVDD69 pKa = 3.53PSNPEE74 pKa = 3.66SVRR77 pKa = 11.84EE78 pKa = 4.13IISTVYY84 pKa = 8.37EE85 pKa = 3.94TLFNKK90 pKa = 9.55TYY92 pKa = 10.63QEE94 pKa = 4.48DD95 pKa = 4.04PQGVDD100 pKa = 2.35SWVDD104 pKa = 3.36LVVSGQQSLGEE115 pKa = 4.43AIAGITYY122 pKa = 9.73IGEE125 pKa = 4.72GIATKK130 pKa = 9.93PDD132 pKa = 3.12EE133 pKa = 4.26YY134 pKa = 10.82RR135 pKa = 11.84PYY137 pKa = 10.65FATDD141 pKa = 3.21DD142 pKa = 4.48DD143 pKa = 4.63FNKK146 pKa = 10.65AYY148 pKa = 10.31VAAKK152 pKa = 10.09AYY154 pKa = 7.72EE155 pKa = 4.15AKK157 pKa = 10.15KK158 pKa = 10.65DD159 pKa = 3.56AALEE163 pKa = 4.11VADD166 pKa = 4.47TIEE169 pKa = 4.05NVKK172 pKa = 10.04VDD174 pKa = 3.94KK175 pKa = 10.13DD176 pKa = 3.88TLQKK180 pKa = 8.92MQEE183 pKa = 4.04IIEE186 pKa = 4.3DD187 pKa = 3.92VKK189 pKa = 11.58DD190 pKa = 3.57EE191 pKa = 4.22TDD193 pKa = 3.38LDD195 pKa = 4.11EE196 pKa = 4.84VKK198 pKa = 11.0NIADD202 pKa = 3.66YY203 pKa = 11.35LKK205 pKa = 10.86DD206 pKa = 3.29IVEE209 pKa = 4.82DD210 pKa = 4.2PNTVSSNDD218 pKa = 3.69LNDD221 pKa = 3.57QNSSQIQNNLSDD233 pKa = 3.72DD234 pKa = 4.38TYY236 pKa = 11.75NDD238 pKa = 3.4AYY240 pKa = 10.65GYY242 pKa = 11.21NFDD245 pKa = 3.74TDD247 pKa = 3.33AWPSIMMEE255 pKa = 4.06LQMLPVEE262 pKa = 4.55GRR264 pKa = 11.84VEE266 pKa = 4.04LVNLMKK272 pKa = 10.64EE273 pKa = 4.21AVDD276 pKa = 4.24KK277 pKa = 11.13YY278 pKa = 11.39LSLAQANVPVTQEE291 pKa = 4.08YY292 pKa = 10.36LEE294 pKa = 4.0QWAALDD300 pKa = 3.99NEE302 pKa = 4.82FEE304 pKa = 4.49HH305 pKa = 6.78KK306 pKa = 10.42RR307 pKa = 11.84ADD309 pKa = 3.11IYY311 pKa = 11.03QKK313 pKa = 10.57HH314 pKa = 5.54GLPYY318 pKa = 10.31FIEE321 pKa = 4.26EE322 pKa = 4.36SVADD326 pKa = 4.2EE327 pKa = 4.62SFLNLDD333 pKa = 3.61PNIQRR338 pKa = 11.84TYY340 pKa = 10.05FQLEE344 pKa = 3.85KK345 pKa = 10.85DD346 pKa = 4.06YY347 pKa = 11.4LPKK350 pKa = 10.59LKK352 pKa = 10.48QEE354 pKa = 4.15LQYY357 pKa = 10.84VDD359 pKa = 4.09PMNVDD364 pKa = 2.81SFYY367 pKa = 11.03TLFKK371 pKa = 11.16LEE373 pKa = 4.72DD374 pKa = 3.99EE375 pKa = 4.49MTEE378 pKa = 4.15SQDD381 pKa = 3.39KK382 pKa = 9.02LTSGVGPTYY391 pKa = 10.38IPEE394 pKa = 4.21DD395 pKa = 3.35NYY397 pKa = 11.41YY398 pKa = 11.16DD399 pKa = 4.21FDD401 pKa = 3.82NTQNSMDD408 pKa = 4.14NYY410 pKa = 10.46YY411 pKa = 10.9NDD413 pKa = 3.62QISYY417 pKa = 11.14GDD419 pKa = 4.68LATADD424 pKa = 4.23NNALDD429 pKa = 4.24NDD431 pKa = 4.37LTTTTQTDD439 pKa = 3.62NYY441 pKa = 9.36NANAMSALEE450 pKa = 4.96DD451 pKa = 3.59MMASYY456 pKa = 10.2YY457 pKa = 10.72AQGGAYY463 pKa = 9.13CDD465 pKa = 4.0NIIASKK471 pKa = 9.08LTSTLSQDD479 pKa = 3.68QLVSLAKK486 pKa = 10.41DD487 pKa = 2.94IQNYY491 pKa = 8.95VFTSPYY497 pKa = 9.72FQDD500 pKa = 3.46GQITYY505 pKa = 10.8DD506 pKa = 3.61EE507 pKa = 4.43YY508 pKa = 11.64QPIANDD514 pKa = 3.82MIQIYY519 pKa = 10.08NKK521 pKa = 10.57YY522 pKa = 10.52GIDD525 pKa = 4.11LMDD528 pKa = 5.05LASDD532 pKa = 5.11CYY534 pKa = 11.43AIMM537 pKa = 5.07

Molecular weight: 60.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6Q3D3|A6Q3D3_NITSB Putative membrane protein insertion efficiency factor OS=Nitratiruptor sp. (strain SB155-2) OX=387092 GN=NIS_0880 PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.44NTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 7.5RR14 pKa = 11.84THH16 pKa = 5.97GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.78TKK25 pKa = 10.46NGRR28 pKa = 11.84KK29 pKa = 9.26VINARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.61GRR39 pKa = 11.84KK40 pKa = 8.79RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1843

0

1843

587077

37

1507

318.5

36.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.983 ± 0.058

0.875 ± 0.021

5.325 ± 0.046

7.473 ± 0.071

5.144 ± 0.053

5.968 ± 0.052

2.07 ± 0.025

8.501 ± 0.057

8.914 ± 0.067

9.936 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.356 ± 0.03

4.252 ± 0.046

3.589 ± 0.032

3.375 ± 0.032

3.968 ± 0.035

5.426 ± 0.043

4.633 ± 0.041

6.285 ± 0.042

0.858 ± 0.021

4.069 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski