Pseudomonas virus F116

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Hollowayvirus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5QF89|Q5QF89_9CAUD Uncharacterized protein OS=Pseudomonas virus F116 OX=280701 GN=21 PE=4 SV=1
MM1 pKa = 6.67MWTYY5 pKa = 11.07RR6 pKa = 11.84EE7 pKa = 3.91RR8 pKa = 11.84HH9 pKa = 5.13NRR11 pKa = 11.84AAFNCAQQAWDD22 pKa = 4.29RR23 pKa = 11.84EE24 pKa = 4.2VDD26 pKa = 4.05PLWDD30 pKa = 3.65QPDD33 pKa = 4.07PEE35 pKa = 6.11PEE37 pKa = 4.25DD38 pKa = 4.22EE39 pKa = 4.26EE40 pKa = 5.61QEE42 pKa = 5.18DD43 pKa = 3.93EE44 pKa = 6.16DD45 pKa = 4.33GLQRR49 pKa = 4.66

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5QF36|Q5QF36_9CAUD Uncharacterized protein OS=Pseudomonas virus F116 OX=280701 GN=4 PE=4 SV=1
MM1 pKa = 7.08KK2 pKa = 10.05QAPSTMQNEE11 pKa = 4.62TLGHH15 pKa = 6.32IRR17 pKa = 11.84AFWAEE22 pKa = 3.93KK23 pKa = 10.48GYY25 pKa = 11.18APTVAEE31 pKa = 4.49LAAKK35 pKa = 10.42AGVRR39 pKa = 11.84QFAIQQRR46 pKa = 11.84LTALEE51 pKa = 3.97NKK53 pKa = 10.21GYY55 pKa = 9.67IQRR58 pKa = 11.84DD59 pKa = 3.59PKK61 pKa = 9.84VARR64 pKa = 11.84SIRR67 pKa = 11.84PLL69 pKa = 3.42

Molecular weight: 7.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

20200

30

2129

288.6

31.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.302 ± 0.532

1.059 ± 0.199

6.183 ± 0.184

6.109 ± 0.291

3.02 ± 0.185

8.134 ± 0.291

2.089 ± 0.164

4.129 ± 0.218

3.817 ± 0.273

7.891 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.337 ± 0.164

2.896 ± 0.148

5.861 ± 0.366

5.139 ± 0.263

7.861 ± 0.36

5.322 ± 0.191

5.421 ± 0.21

6.292 ± 0.244

1.614 ± 0.165

2.525 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski