Pseudopropionibacterium propionicum (strain F0230a) (Propionibacterium propionicum)
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2931 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6X1H9|I6X1H9_PSEPQ WYL domain-containing protein OS=Pseudopropionibacterium propionicum (strain F0230a) OX=767029 GN=HMPREF9154_2000 PE=4 SV=1
MM1 pKa = 7.57 TYY3 pKa = 10.0 IIALPCVDD11 pKa = 3.84 VKK13 pKa = 11.27 DD14 pKa = 3.97 RR15 pKa = 11.84 ACVEE19 pKa = 4.23 EE20 pKa = 5.05 CPVDD24 pKa = 4.7 CIYY27 pKa = 11.01 EE28 pKa = 4.32 GNRR31 pKa = 11.84 MLYY34 pKa = 9.14 IHH36 pKa = 7.22 PEE38 pKa = 3.86 EE39 pKa = 4.98 CVDD42 pKa = 4.78 CGACEE47 pKa = 3.85 PVCPVEE53 pKa = 4.4 AIYY56 pKa = 11.22 YY57 pKa = 10.23 EE58 pKa = 4.89 DD59 pKa = 5.66 DD60 pKa = 3.77 LPEE63 pKa = 4.39 DD64 pKa = 3.37 QEE66 pKa = 4.47 EE67 pKa = 4.27 YY68 pKa = 11.44 LDD70 pKa = 3.72 INAQFFEE77 pKa = 4.88 DD78 pKa = 3.92 LGSPGGAARR87 pKa = 11.84 LGPVDD92 pKa = 4.07 HH93 pKa = 7.42 DD94 pKa = 4.08 HH95 pKa = 6.77 PTVEE99 pKa = 3.97 QLPPQGEE106 pKa = 4.11
Molecular weight: 11.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.899
IPC2_protein 3.783
IPC_protein 3.732
Toseland 3.541
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.923
Wikipedia 3.63
Rodwell 3.567
Grimsley 3.465
Solomon 3.694
Lehninger 3.656
Nozaki 3.834
DTASelect 3.999
Thurlkill 3.592
EMBOSS 3.643
Sillero 3.859
Patrickios 0.006
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.808
Protein with the highest isoelectric point:
>tr|I6WTL8|I6WTL8_PSEPQ FeS_assembly_P domain-containing protein OS=Pseudopropionibacterium propionicum (strain F0230a) OX=767029 GN=HMPREF9154_1086 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SRR15 pKa = 11.84 THH17 pKa = 6.17 GFRR20 pKa = 11.84 SRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILSARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.98 GRR40 pKa = 11.84 TEE42 pKa = 3.99 LSAA45 pKa = 4.86
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.464
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.076
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.14
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2931
0
2931
942163
37
2730
321.4
34.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.728 ± 0.057
0.837 ± 0.014
5.658 ± 0.035
6.034 ± 0.04
3.111 ± 0.027
8.948 ± 0.039
2.117 ± 0.02
4.491 ± 0.032
2.604 ± 0.036
10.607 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.137 ± 0.019
2.361 ± 0.022
5.453 ± 0.027
2.972 ± 0.026
7.458 ± 0.048
5.613 ± 0.035
5.959 ± 0.037
8.459 ± 0.042
1.564 ± 0.018
1.888 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here