Amino acid dipepetide frequency for Clostridium saccharobutylicum DSM 13864

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.861AlaAla: 3.861 ± 0.079	0.722AlaCys: 0.722 ± 0.024	2.69AlaAsp: 2.69 ± 0.053	3.39AlaGlu: 3.39 ± 0.054	2.428AlaPhe: 2.428 ± 0.054	3.572AlaGly: 3.572 ± 0.066	0.8AlaHis: 0.8 ± 0.027	5.68AlaIle: 5.68 ± 0.068	4.615AlaLys: 4.615 ± 0.07	5.418AlaLeu: 5.418 ± 0.069	1.624AlaMet: 1.624 ± 0.038	2.921AlaAsn: 2.921 ± 0.055	1.34AlaPro: 1.34 ± 0.032	1.558AlaGln: 1.558 ± 0.037	1.743AlaArg: 1.743 ± 0.04	3.518AlaSer: 3.518 ± 0.062	3.037AlaThr: 3.037 ± 0.068	3.808AlaVal: 3.808 ± 0.066	0.365AlaTrp: 0.365 ± 0.016	2.047AlaTyr: 2.047 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	0.685CysAla: 0.685 ± 0.024	0.22CysCys: 0.22 ± 0.016	0.794CysAsp: 0.794 ± 0.028	0.854CysGlu: 0.854 ± 0.032	0.547CysPhe: 0.547 ± 0.021	1.17CysGly: 1.17 ± 0.031	0.214CysHis: 0.214 ± 0.013	1.267CysIle: 1.267 ± 0.037	1.065CysLys: 1.065 ± 0.031	0.958CysLeu: 0.958 ± 0.03	0.318CysMet: 0.318 ± 0.015	0.798CysAsn: 0.798 ± 0.026	0.463CysPro: 0.463 ± 0.023	0.231CysGln: 0.231 ± 0.015	0.399CysArg: 0.399 ± 0.02	0.825CysSer: 0.825 ± 0.026	0.625CysThr: 0.625 ± 0.024	0.673CysVal: 0.673 ± 0.024	0.081CysTrp: 0.081 ± 0.008	0.48CysTyr: 0.48 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	2.874AspAla: 2.874 ± 0.053	0.638AspCys: 0.638 ± 0.024	3.188AspAsp: 3.188 ± 0.054	4.936AspGlu: 4.936 ± 0.073	2.715AspPhe: 2.715 ± 0.045	3.751AspGly: 3.751 ± 0.068	0.551AspHis: 0.551 ± 0.024	6.12AspIle: 6.12 ± 0.074	5.588AspLys: 5.588 ± 0.067	4.781AspLeu: 4.781 ± 0.065	1.468AspMet: 1.468 ± 0.033	3.792AspAsn: 3.792 ± 0.062	1.251AspPro: 1.251 ± 0.033	0.951AspGln: 0.951 ± 0.028	1.714AspArg: 1.714 ± 0.039	3.447AspSer: 3.447 ± 0.055	2.598AspThr: 2.598 ± 0.042	3.594AspVal: 3.594 ± 0.061	0.381AspTrp: 0.381 ± 0.021	2.645AspTyr: 2.645 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	3.854GluAla: 3.854 ± 0.054	0.875GluCys: 0.875 ± 0.025	4.202GluAsp: 4.202 ± 0.062	6.679GluGlu: 6.679 ± 0.089	3.116GluPhe: 3.116 ± 0.059	3.879GluGly: 3.879 ± 0.059	0.983GluHis: 0.983 ± 0.029	7.434GluIle: 7.434 ± 0.086	7.395GluLys: 7.395 ± 0.097	6.69GluLeu: 6.69 ± 0.078	1.97GluMet: 1.97 ± 0.043	5.498GluAsn: 5.498 ± 0.08	1.258GluPro: 1.258 ± 0.034	2.102GluGln: 2.102 ± 0.051	2.515GluArg: 2.515 ± 0.049	3.852GluSer: 3.852 ± 0.061	2.962GluThr: 2.962 ± 0.049	4.645GluVal: 4.645 ± 0.063	0.52GluTrp: 0.52 ± 0.018	3.095GluTyr: 3.095 ± 0.061	0.0GluXaa: 0.0 ± 0.0
Phe	2.188PheAla: 2.188 ± 0.049	0.527PheCys: 0.527 ± 0.017	2.592PheAsp: 2.592 ± 0.041	2.84PheGlu: 2.84 ± 0.046	1.85PhePhe: 1.85 ± 0.042	2.775PheGly: 2.775 ± 0.054	0.534PheHis: 0.534 ± 0.018	4.39PheIle: 4.39 ± 0.07	3.82PheLys: 3.82 ± 0.057	3.686PheLeu: 3.686 ± 0.059	1.064PheMet: 1.064 ± 0.03	3.02PheAsn: 3.02 ± 0.05	1.093PhePro: 1.093 ± 0.033	0.925PheGln: 0.925 ± 0.027	1.242PheArg: 1.242 ± 0.03	3.058PheSer: 3.058 ± 0.05	2.339PheThr: 2.339 ± 0.042	2.535PheVal: 2.535 ± 0.046	0.279PheTrp: 0.279 ± 0.015	1.802PheTyr: 1.802 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	3.91GlyAla: 3.91 ± 0.073	0.926GlyCys: 0.926 ± 0.031	3.163GlyAsp: 3.163 ± 0.056	4.14GlyGlu: 4.14 ± 0.069	2.816GlyPhe: 2.816 ± 0.048	4.106GlyGly: 4.106 ± 0.076	0.919GlyHis: 0.919 ± 0.029	6.809GlyIle: 6.809 ± 0.093	5.349GlyLys: 5.349 ± 0.059	5.103GlyLeu: 5.103 ± 0.073	1.784GlyMet: 1.784 ± 0.037	3.502GlyAsn: 3.502 ± 0.068	1.066GlyPro: 1.066 ± 0.03	1.601GlyGln: 1.601 ± 0.038	2.007GlyArg: 2.007 ± 0.039	3.673GlySer: 3.673 ± 0.06	3.595GlyThr: 3.595 ± 0.071	4.384GlyVal: 4.384 ± 0.073	0.672GlyTrp: 0.672 ± 0.033	2.958GlyTyr: 2.958 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	0.652HisAla: 0.652 ± 0.022	0.214HisCys: 0.214 ± 0.013	0.708HisAsp: 0.708 ± 0.026	0.915HisGlu: 0.915 ± 0.028	0.603HisPhe: 0.603 ± 0.021	0.95HisGly: 0.95 ± 0.026	0.277HisHis: 0.277 ± 0.019	1.324HisIle: 1.324 ± 0.033	1.09HisLys: 1.09 ± 0.028	1.131HisLeu: 1.131 ± 0.028	0.347HisMet: 0.347 ± 0.016	0.859HisAsn: 0.859 ± 0.024	0.523HisPro: 0.523 ± 0.02	0.327HisGln: 0.327 ± 0.016	0.481HisArg: 0.481 ± 0.019	0.88HisSer: 0.88 ± 0.023	0.67HisThr: 0.67 ± 0.025	0.71HisVal: 0.71 ± 0.024	0.087HisTrp: 0.087 ± 0.008	0.574HisTyr: 0.574 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	5.817IleAla: 5.817 ± 0.075	1.414IleCys: 1.414 ± 0.031	6.004IleAsp: 6.004 ± 0.077	7.187IleGlu: 7.187 ± 0.084	4.049IlePhe: 4.049 ± 0.072	6.167IleGly: 6.167 ± 0.087	1.216IleHis: 1.216 ± 0.034	9.569IleIle: 9.569 ± 0.12	9.071IleLys: 9.071 ± 0.098	9.032IleLeu: 9.032 ± 0.099	2.326IleMet: 2.326 ± 0.047	6.994IleAsn: 6.994 ± 0.093	3.123IlePro: 3.123 ± 0.054	2.467IleGln: 2.467 ± 0.041	2.956IleArg: 2.956 ± 0.045	7.179IleSer: 7.179 ± 0.092	5.133IleThr: 5.133 ± 0.061	5.993IleVal: 5.993 ± 0.091	0.551IleTrp: 0.551 ± 0.025	3.62IleTyr: 3.62 ± 0.07	0.0IleXaa: 0.0 ± 0.0
Lys	4.643LysAla: 4.643 ± 0.068	1.032LysCys: 1.032 ± 0.034	5.86LysAsp: 5.86 ± 0.072	8.503LysGlu: 8.503 ± 0.094	3.36LysPhe: 3.36 ± 0.053	4.768LysGly: 4.768 ± 0.065	1.181LysHis: 1.181 ± 0.031	8.721LysIle: 8.721 ± 0.099	8.094LysLys: 8.094 ± 0.089	7.79LysLeu: 7.79 ± 0.079	2.411LysMet: 2.411 ± 0.042	6.603LysAsn: 6.603 ± 0.074	1.971LysPro: 1.971 ± 0.041	2.519LysGln: 2.519 ± 0.044	2.965LysArg: 2.965 ± 0.044	5.556LysSer: 5.556 ± 0.067	4.124LysThr: 4.124 ± 0.056	5.842LysVal: 5.842 ± 0.077	0.67LysTrp: 0.67 ± 0.024	4.079LysTyr: 4.079 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	4.804LeuAla: 4.804 ± 0.066	1.179LeuCys: 1.179 ± 0.035	5.158LeuAsp: 5.158 ± 0.059	6.204LeuGlu: 6.204 ± 0.087	3.553LeuPhe: 3.553 ± 0.057	5.675LeuGly: 5.675 ± 0.088	1.138LeuHis: 1.138 ± 0.029	8.291LeuIle: 8.291 ± 0.095	8.389LeuLys: 8.389 ± 0.079	7.482LeuLeu: 7.482 ± 0.096	2.295LeuMet: 2.295 ± 0.047	6.213LeuAsn: 6.213 ± 0.074	2.577LeuPro: 2.577 ± 0.048	2.226LeuGln: 2.226 ± 0.048	2.9LeuArg: 2.9 ± 0.047	6.644LeuSer: 6.644 ± 0.092	4.581LeuThr: 4.581 ± 0.062	5.092LeuVal: 5.092 ± 0.062	0.531LeuTrp: 0.531 ± 0.021	3.127LeuTyr: 3.127 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	1.687MetAla: 1.687 ± 0.043	0.306MetCys: 0.306 ± 0.016	1.423MetAsp: 1.423 ± 0.034	1.78MetGlu: 1.78 ± 0.038	1.06MetPhe: 1.06 ± 0.028	1.599MetGly: 1.599 ± 0.039	0.352MetHis: 0.352 ± 0.016	2.341MetIle: 2.341 ± 0.043	2.653MetLys: 2.653 ± 0.046	2.213MetLeu: 2.213 ± 0.047	0.697MetMet: 0.697 ± 0.028	1.885MetAsn: 1.885 ± 0.04	0.889MetPro: 0.889 ± 0.025	0.762MetGln: 0.762 ± 0.023	0.809MetArg: 0.809 ± 0.025	1.746MetSer: 1.746 ± 0.039	1.201MetThr: 1.201 ± 0.033	1.504MetVal: 1.504 ± 0.037	0.155MetTrp: 0.155 ± 0.012	0.893MetTyr: 0.893 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	3.221AsnAla: 3.221 ± 0.051	0.838AsnCys: 0.838 ± 0.028	3.697AsnAsp: 3.697 ± 0.058	5.013AsnGlu: 5.013 ± 0.077	2.607AsnPhe: 2.607 ± 0.045	4.142AsnGly: 4.142 ± 0.065	0.846AsnHis: 0.846 ± 0.027	7.334AsnIle: 7.334 ± 0.084	6.541AsnLys: 6.541 ± 0.083	6.092AsnLeu: 6.092 ± 0.091	1.717AsnMet: 1.717 ± 0.037	5.268AsnAsn: 5.268 ± 0.076	2.055AsnPro: 2.055 ± 0.044	1.653AsnGln: 1.653 ± 0.041	1.981AsnArg: 1.981 ± 0.039	4.517AsnSer: 4.517 ± 0.076	3.211AsnThr: 3.211 ± 0.053	4.131AsnVal: 4.131 ± 0.061	0.528AsnTrp: 0.528 ± 0.024	2.826AsnTyr: 2.826 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	1.317ProAla: 1.317 ± 0.034	0.319ProCys: 0.319 ± 0.014	1.345ProAsp: 1.345 ± 0.038	1.941ProGlu: 1.941 ± 0.046	1.245ProPhe: 1.245 ± 0.028	1.493ProGly: 1.493 ± 0.035	0.436ProHis: 0.436 ± 0.02	2.616ProIle: 2.616 ± 0.05	2.133ProLys: 2.133 ± 0.039	2.286ProLeu: 2.286 ± 0.043	0.663ProMet: 0.663 ± 0.023	1.615ProAsn: 1.615 ± 0.036	0.496ProPro: 0.496 ± 0.019	0.773ProGln: 0.773 ± 0.026	0.767ProArg: 0.767 ± 0.025	1.753ProSer: 1.753 ± 0.045	1.411ProThr: 1.411 ± 0.033	1.889ProVal: 1.889 ± 0.04	0.217ProTrp: 0.217 ± 0.013	1.148ProTyr: 1.148 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	1.477GlnAla: 1.477 ± 0.037	0.283GlnCys: 0.283 ± 0.017	1.383GlnAsp: 1.383 ± 0.035	1.861GlnGlu: 1.861 ± 0.043	0.977GlnPhe: 0.977 ± 0.025	1.611GlnGly: 1.611 ± 0.04	0.315GlnHis: 0.315 ± 0.016	2.434GlnIle: 2.434 ± 0.047	2.294GlnLys: 2.294 ± 0.04	2.182GlnLeu: 2.182 ± 0.046	0.695GlnMet: 0.695 ± 0.023	1.848GlnAsn: 1.848 ± 0.038	0.587GlnPro: 0.587 ± 0.024	0.789GlnGln: 0.789 ± 0.029	0.899GlnArg: 0.899 ± 0.033	1.62GlnSer: 1.62 ± 0.035	1.282GlnThr: 1.282 ± 0.037	1.602GlnVal: 1.602 ± 0.038	0.228GlnTrp: 0.228 ± 0.013	1.165GlnTyr: 1.165 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	1.738ArgAla: 1.738 ± 0.04	0.417ArgCys: 0.417 ± 0.019	1.742ArgAsp: 1.742 ± 0.038	2.5ArgGlu: 2.5 ± 0.049	1.37ArgPhe: 1.37 ± 0.03	1.8ArgGly: 1.8 ± 0.038	0.431ArgHis: 0.431 ± 0.017	3.131ArgIle: 3.131 ± 0.048	2.974ArgLys: 2.974 ± 0.048	2.747ArgLeu: 2.747 ± 0.047	0.916ArgMet: 0.916 ± 0.028	2.197ArgAsn: 2.197 ± 0.041	0.745ArgPro: 0.745 ± 0.025	0.855ArgGln: 0.855 ± 0.027	1.312ArgArg: 1.312 ± 0.036	1.571ArgSer: 1.571 ± 0.035	1.517ArgThr: 1.517 ± 0.031	2.076ArgVal: 2.076 ± 0.044	0.249ArgTrp: 0.249 ± 0.015	1.346ArgTyr: 1.346 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	3.352SerAla: 3.352 ± 0.052	0.698SerCys: 0.698 ± 0.027	3.65SerAsp: 3.65 ± 0.053	4.297SerGlu: 4.297 ± 0.068	3.006SerPhe: 3.006 ± 0.048	4.333SerGly: 4.333 ± 0.066	0.872SerHis: 0.872 ± 0.025	6.742SerIle: 6.742 ± 0.091	5.954SerLys: 5.954 ± 0.081	5.906SerLeu: 5.906 ± 0.073	1.717SerMet: 1.717 ± 0.038	4.497SerAsn: 4.497 ± 0.063	1.525SerPro: 1.525 ± 0.036	1.77SerGln: 1.77 ± 0.037	2.013SerArg: 2.013 ± 0.04	4.814SerSer: 4.814 ± 0.084	3.484SerThr: 3.484 ± 0.059	3.945SerVal: 3.945 ± 0.062	0.45SerTrp: 0.45 ± 0.017	2.716SerTyr: 2.716 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	2.971ThrAla: 2.971 ± 0.055	0.562ThrCys: 0.562 ± 0.022	2.648ThrAsp: 2.648 ± 0.05	2.992ThrGlu: 2.992 ± 0.05	2.229ThrPhe: 2.229 ± 0.038	3.699ThrGly: 3.699 ± 0.068	0.755ThrHis: 0.755 ± 0.024	4.922ThrIle: 4.922 ± 0.06	3.891ThrLys: 3.891 ± 0.057	4.787ThrLeu: 4.787 ± 0.068	1.166ThrMet: 1.166 ± 0.031	3.229ThrAsn: 3.229 ± 0.058	1.716ThrPro: 1.716 ± 0.043	1.382ThrGln: 1.382 ± 0.03	1.464ThrArg: 1.464 ± 0.033	3.397ThrSer: 3.397 ± 0.068	2.939ThrThr: 2.939 ± 0.066	3.322ThrVal: 3.322 ± 0.064	0.402ThrTrp: 0.402 ± 0.019	2.046ThrTyr: 2.046 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	3.801ValAla: 3.801 ± 0.076	0.839ValCys: 0.839 ± 0.028	3.774ValAsp: 3.774 ± 0.058	4.201ValGlu: 4.201 ± 0.063	2.713ValPhe: 2.713 ± 0.045	3.941ValGly: 3.941 ± 0.061	0.841ValHis: 0.841 ± 0.025	6.031ValIle: 6.031 ± 0.075	5.466ValLys: 5.466 ± 0.069	5.483ValLeu: 5.483 ± 0.067	1.567ValMet: 1.567 ± 0.033	3.915ValAsn: 3.915 ± 0.052	1.861ValPro: 1.861 ± 0.043	1.606ValGln: 1.606 ± 0.039	1.856ValArg: 1.856 ± 0.041	4.412ValSer: 4.412 ± 0.058	3.424ValThr: 3.424 ± 0.068	4.244ValVal: 4.244 ± 0.069	0.374ValTrp: 0.374 ± 0.016	2.343ValTyr: 2.343 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.365TrpAla: 0.365 ± 0.018	0.094TrpCys: 0.094 ± 0.009	0.387TrpAsp: 0.387 ± 0.02	0.406TrpGlu: 0.406 ± 0.02	0.349TrpPhe: 0.349 ± 0.016	0.469TrpGly: 0.469 ± 0.018	0.113TrpHis: 0.113 ± 0.01	0.754TrpIle: 0.754 ± 0.027	0.54TrpLys: 0.54 ± 0.023	0.559TrpLeu: 0.559 ± 0.024	0.204TrpMet: 0.204 ± 0.013	0.546TrpAsn: 0.546 ± 0.023	0.143TrpPro: 0.143 ± 0.01	0.23TrpGln: 0.23 ± 0.014	0.236TrpArg: 0.236 ± 0.014	0.436TrpSer: 0.436 ± 0.021	0.342TrpThr: 0.342 ± 0.016	0.391TrpVal: 0.391 ± 0.017	0.097TrpTrp: 0.097 ± 0.009	0.474TrpTyr: 0.474 ± 0.03	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.919TyrAla: 1.919 ± 0.039	0.562TyrCys: 0.562 ± 0.023	2.574TyrAsp: 2.574 ± 0.065	2.884TyrGlu: 2.884 ± 0.054	1.966TyrPhe: 1.966 ± 0.046	2.583TyrGly: 2.583 ± 0.046	0.549TyrHis: 0.549 ± 0.022	3.958TyrIle: 3.958 ± 0.06	3.773TyrLys: 3.773 ± 0.064	3.734TyrLeu: 3.734 ± 0.069	1.007TyrMet: 1.007 ± 0.029	3.018TyrAsn: 3.018 ± 0.047	1.18TyrPro: 1.18 ± 0.031	0.805TyrGln: 0.805 ± 0.025	1.376TyrArg: 1.376 ± 0.034	2.843TyrSer: 2.843 ± 0.05	2.043TyrThr: 2.043 ± 0.052	2.313TyrVal: 2.313 ± 0.041	0.299TyrTrp: 0.299 ± 0.015	2.036TyrTyr: 2.036 ± 0.06	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4355 proteins (1319205 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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