Yacon necrotic mottle virus
Average proteome isoelectric point is 7.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C4MZL5|A0A0C4MZL5_9VIRU ORF4 OS=Yacon necrotic mottle virus OX=1561150 PE=4 SV=1
MM1 pKa = 8.04 ADD3 pKa = 3.63 RR4 pKa = 11.84 FEE6 pKa = 5.28 KK7 pKa = 10.56 SIKK10 pKa = 9.86 DD11 pKa = 3.56 WYY13 pKa = 10.43 DD14 pKa = 2.96 HH15 pKa = 6.26 SQTSNLEE22 pKa = 4.15 FINPLKK28 pKa = 10.65 NQNQSISEE36 pKa = 4.21 LEE38 pKa = 4.06 NNISCIFDD46 pKa = 3.7 RR47 pKa = 11.84 LSLFAKK53 pKa = 10.28 ISIKK57 pKa = 10.46 NFHH60 pKa = 6.94 KK61 pKa = 10.41 IISHH65 pKa = 6.51 LEE67 pKa = 3.89 SLEE70 pKa = 3.87 KK71 pKa = 10.47 RR72 pKa = 11.84 LEE74 pKa = 4.05 KK75 pKa = 10.94 CEE77 pKa = 3.9 EE78 pKa = 4.11 IQRR81 pKa = 11.84 KK82 pKa = 4.87 TQSEE86 pKa = 4.08 IRR88 pKa = 11.84 KK89 pKa = 9.09 DD90 pKa = 3.45 LQQISSEE97 pKa = 4.0 IHH99 pKa = 5.42 KK100 pKa = 10.44 SKK102 pKa = 10.48 PLTEE106 pKa = 4.15 KK107 pKa = 10.6 QVLSLVNEE115 pKa = 4.69 IAQQPKK121 pKa = 10.21 LVEE124 pKa = 4.08 EE125 pKa = 3.94 QALRR129 pKa = 11.84 LTEE132 pKa = 4.36 DD133 pKa = 4.08 LNSKK137 pKa = 6.75 VQKK140 pKa = 10.69 VEE142 pKa = 4.05 EE143 pKa = 4.33 LLHH146 pKa = 5.5 EE147 pKa = 4.46 VKK149 pKa = 10.88 GLIIGG154 pKa = 3.93
Molecular weight: 17.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.068
IPC2_protein 5.931
IPC_protein 5.995
Toseland 6.389
ProMoST 6.326
Dawson 6.249
Bjellqvist 6.224
Wikipedia 6.262
Rodwell 6.237
Grimsley 6.532
Solomon 6.249
Lehninger 6.249
Nozaki 6.504
DTASelect 6.693
Thurlkill 6.707
EMBOSS 6.678
Sillero 6.62
Patrickios 4.825
IPC_peptide 6.275
IPC2_peptide 6.532
IPC2.peptide.svr19 6.604
Protein with the highest isoelectric point:
>tr|A0A0C4MZQ9|A0A0C4MZQ9_9VIRU Reverse transcriptase OS=Yacon necrotic mottle virus OX=1561150 PE=4 SV=1
MM1 pKa = 7.11 YY2 pKa = 7.96 RR3 pKa = 11.84 TRR5 pKa = 11.84 DD6 pKa = 3.37 RR7 pKa = 11.84 SPGEE11 pKa = 4.08 GSSSDD16 pKa = 3.04 GGNIRR21 pKa = 11.84 RR22 pKa = 11.84 KK23 pKa = 10.36 CEE25 pKa = 3.62 VKK27 pKa = 10.36 QRR29 pKa = 11.84 EE30 pKa = 3.66 EE31 pKa = 3.43 WLRR34 pKa = 11.84 RR35 pKa = 11.84 NNPDD39 pKa = 3.1 FQLLPKK45 pKa = 9.84 IYY47 pKa = 9.83 RR48 pKa = 11.84 GQFSFEE54 pKa = 4.17 EE55 pKa = 4.32 PGALYY60 pKa = 10.09 RR61 pKa = 11.84 HH62 pKa = 5.06 YY63 pKa = 10.47 QIRR66 pKa = 11.84 ANTTPIRR73 pKa = 11.84 LEE75 pKa = 3.77 EE76 pKa = 3.95 SSIYY80 pKa = 10.82 AGVAQQYY87 pKa = 8.65 EE88 pKa = 4.21 RR89 pKa = 11.84 EE90 pKa = 4.14 AAKK93 pKa = 10.44 QAVRR97 pKa = 11.84 TMRR100 pKa = 11.84 HH101 pKa = 4.93 LQAVLDD107 pKa = 5.42 FKK109 pKa = 11.58 AQVCLAKK116 pKa = 10.65 SSADD120 pKa = 3.94 NYY122 pKa = 10.39 WGDD125 pKa = 2.86 HH126 pKa = 5.13 WPNVKK131 pKa = 9.96 KK132 pKa = 10.64 QNQQARR138 pKa = 11.84 KK139 pKa = 9.21 LLSEE143 pKa = 4.7 LEE145 pKa = 4.14 ALCIEE150 pKa = 5.15 LGQVPII156 pKa = 4.55
Molecular weight: 18.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.803
IPC2_protein 8.682
IPC_protein 8.712
Toseland 9.151
ProMoST 9.077
Dawson 9.487
Bjellqvist 9.341
Wikipedia 9.677
Rodwell 9.619
Grimsley 9.545
Solomon 9.589
Lehninger 9.545
Nozaki 9.414
DTASelect 9.268
Thurlkill 9.341
EMBOSS 9.619
Sillero 9.502
Patrickios 4.8
IPC_peptide 9.575
IPC2_peptide 8.185
IPC2.peptide.svr19 7.735
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2484
143
2031
621.0
71.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.354 ± 0.714
1.812 ± 0.387
5.193 ± 0.689
9.138 ± 0.867
2.858 ± 0.154
5.113 ± 0.876
1.973 ± 0.347
7.528 ± 0.669
8.333 ± 0.892
8.011 ± 1.461
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.295 ± 0.686
4.831 ± 0.283
4.791 ± 0.644
5.475 ± 0.835
6.24 ± 0.734
6.562 ± 0.942
5.274 ± 0.841
4.147 ± 0.374
1.49 ± 0.355
3.341 ± 0.531
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here