Mycobacterium phage Brocalys

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142K6J4|A0A142K6J4_9CAUD Minor tail protein OS=Mycobacterium phage Brocalys OX=1815608 GN=20 PE=4 SV=1
MM1 pKa = 7.89AYY3 pKa = 10.34AEE5 pKa = 4.99LLDD8 pKa = 3.81TDD10 pKa = 3.83MSTWCPITLHH20 pKa = 6.07YY21 pKa = 10.5KK22 pKa = 8.71VTDD25 pKa = 3.9GDD27 pKa = 3.94QVSYY31 pKa = 10.88LAVTRR36 pKa = 11.84VSFVTATGRR45 pKa = 11.84VEE47 pKa = 5.67AFACDD52 pKa = 3.61EE53 pKa = 4.2TGLAISLTPVWAIDD67 pKa = 3.44GDD69 pKa = 4.14VGHH72 pKa = 6.94NDD74 pKa = 3.21ALVAAGYY81 pKa = 7.32TVSS84 pKa = 3.59

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142K6N7|A0A142K6N7_9CAUD Uncharacterized protein OS=Mycobacterium phage Brocalys OX=1815608 GN=63 PE=4 SV=1
MM1 pKa = 7.13TVEE4 pKa = 3.8SMLWFRR10 pKa = 11.84ARR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84SHH16 pKa = 6.55RR17 pKa = 11.84SAWGHH22 pKa = 5.8PRR24 pKa = 11.84PPAPPKK30 pKa = 9.37PQPTQGEE37 pKa = 4.39PMSNLTPEE45 pKa = 4.02QLEE48 pKa = 4.41AIAYY52 pKa = 8.8IVLAFTGPPSLAYY65 pKa = 10.09FLVKK69 pKa = 10.51GLFRR73 pKa = 6.02

Molecular weight: 8.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

17633

25

1183

183.7

20.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.577 ± 0.451

1.27 ± 0.177

6.839 ± 0.213

6.062 ± 0.306

2.96 ± 0.18

8.365 ± 0.537

2.359 ± 0.212

4.31 ± 0.184

3.448 ± 0.171

7.537 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.11

3.165 ± 0.15

6.131 ± 0.232

3.579 ± 0.156

7.174 ± 0.374

5.541 ± 0.203

6.522 ± 0.255

7.248 ± 0.267

2.24 ± 0.143

2.405 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski