Aspergillus coremiiformis
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9078 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N6ZBR7|A0A5N6ZBR7_9EURO Major facilitator superfamily domain-containing protein OS=Aspergillus coremiiformis OX=138285 GN=BDV28DRAFT_164074 PE=4 SV=1
MM1 pKa = 7.41 KK2 pKa = 10.38 FSSTSALAAALVAGVNGASIPRR24 pKa = 11.84 TQGSVPITFIGAADD38 pKa = 3.6 AQFTQDD44 pKa = 4.1 FPTDD48 pKa = 3.52 GSGTAITNPLSISHH62 pKa = 7.03 ISSSTDD68 pKa = 2.88 GVSCVFNGIDD78 pKa = 3.28 NSVTVVSGSEE88 pKa = 4.23 TVDD91 pKa = 2.98 VGPPQTQTSGTCNVGSAQPEE111 pKa = 4.4 SRR113 pKa = 11.84 SGTGQVDD120 pKa = 3.11 ITFIGAADD128 pKa = 3.57 AQFTQGFPTDD138 pKa = 3.8 GSSITISNPLSISHH152 pKa = 6.89 IASSTEE158 pKa = 3.98 GVSCTFNGVDD168 pKa = 3.42 NSVTSVTGAQTVDD181 pKa = 3.17 VGPPQTQVSGSCHH194 pKa = 6.5 SII196 pKa = 3.28
Molecular weight: 19.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.897
IPC_protein 3.859
Toseland 3.63
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.859
Rodwell 3.681
Grimsley 3.554
Solomon 3.859
Lehninger 3.808
Nozaki 3.999
DTASelect 4.279
Thurlkill 3.719
EMBOSS 3.859
Sillero 3.986
Patrickios 1.939
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.88
Protein with the highest isoelectric point:
>tr|A0A5N6Z6M9|A0A5N6Z6M9_9EURO S-adenosyl-L-methionine-dependent methyltransferase OS=Aspergillus coremiiformis OX=138285 GN=BDV28DRAFT_114710 PE=4 SV=1
MM1 pKa = 7.88 PSHH4 pKa = 6.91 KK5 pKa = 10.39 SFRR8 pKa = 11.84 TKK10 pKa = 10.45 QKK12 pKa = 9.84 LAKK15 pKa = 9.55 AQKK18 pKa = 8.59 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.14 WRR45 pKa = 11.84 KK46 pKa = 7.51 TRR48 pKa = 11.84 LGLL51 pKa = 4.09
Molecular weight: 6.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9078
0
9078
4351296
49
5990
479.3
53.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.061 ± 0.023
1.263 ± 0.009
5.609 ± 0.017
6.132 ± 0.023
3.788 ± 0.015
6.648 ± 0.021
2.481 ± 0.011
5.006 ± 0.017
4.668 ± 0.021
9.123 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.145 ± 0.01
3.706 ± 0.012
6.108 ± 0.026
4.09 ± 0.018
6.305 ± 0.022
8.493 ± 0.03
5.948 ± 0.015
6.167 ± 0.017
1.431 ± 0.009
2.826 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here