Duck hepatitis B virus (strain China) (DHBV)
Average proteome isoelectric point is 8.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P30029-2|HBSAG-2_HPBDC Isoform of P30029 Isoform S of Large envelope protein OS=Duck hepatitis B virus (strain China) OX=31510 GN=S PE=4 SV=2
MM1 pKa = 7.15 GQQPAKK7 pKa = 10.78 SMDD10 pKa = 2.92 VRR12 pKa = 11.84 RR13 pKa = 11.84 IEE15 pKa = 4.32 GGEE18 pKa = 3.69 ILLNQLAGRR27 pKa = 11.84 MIPKK31 pKa = 10.26 GAVTWSGKK39 pKa = 10.37 YY40 pKa = 7.88 PTIDD44 pKa = 3.16 HH45 pKa = 6.9 LLDD48 pKa = 3.59 HH49 pKa = 6.22 VQTMEE54 pKa = 5.18 EE55 pKa = 4.51 INTLQQQGAWPAGAGRR71 pKa = 11.84 RR72 pKa = 11.84 VGLTNPTPQEE82 pKa = 3.7 IPQPQWTPEE91 pKa = 3.83 EE92 pKa = 4.01 DD93 pKa = 3.23 QKK95 pKa = 11.68 ARR97 pKa = 11.84 EE98 pKa = 3.95 AFRR101 pKa = 11.84 RR102 pKa = 11.84 YY103 pKa = 8.96 QEE105 pKa = 3.91 EE106 pKa = 4.36 RR107 pKa = 11.84 PPEE110 pKa = 4.17 TTTIPPSSTPPWKK123 pKa = 10.18 LQPGDD128 pKa = 4.29 DD129 pKa = 4.29 PLLEE133 pKa = 4.48 SKK135 pKa = 10.74 SLLEE139 pKa = 3.94 THH141 pKa = 7.36 PLYY144 pKa = 10.71 QNPEE148 pKa = 3.83 PAVPVIKK155 pKa = 9.85 TPPLKK160 pKa = 10.58 KK161 pKa = 10.34 KK162 pKa = 10.19 MSGTFGGILAGLIGLLVSFFLLIKK186 pKa = 10.18 ILEE189 pKa = 4.25 ILRR192 pKa = 11.84 KK193 pKa = 9.72 LDD195 pKa = 3.2 WWWISLSSPKK205 pKa = 10.95 GKK207 pKa = 8.31 MQCAFQDD214 pKa = 3.1 TGAQISPHH222 pKa = 5.65 YY223 pKa = 10.44 AGSCPWGCPGFLWTYY238 pKa = 10.39 LRR240 pKa = 11.84 LFIIFLLILLVAAGLLYY257 pKa = 10.14 LTDD260 pKa = 3.77 NGSTILGKK268 pKa = 9.65 LQWASVSALFSSISSLLPSDD288 pKa = 3.65 QKK290 pKa = 11.76 SLVALMFGLLLIWMTSSSATQTLVTLTQLATLSALFFKK328 pKa = 10.98 NN329 pKa = 3.05
Molecular weight: 36.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.566
IPC2_protein 7.819
IPC_protein 7.849
Toseland 7.951
ProMoST 8.258
Dawson 8.565
Bjellqvist 8.682
Wikipedia 8.536
Rodwell 8.609
Grimsley 7.746
Solomon 8.785
Lehninger 8.785
Nozaki 8.887
DTASelect 8.521
Thurlkill 8.609
EMBOSS 8.785
Sillero 8.873
Patrickios 4.724
IPC_peptide 8.785
IPC2_peptide 7.483
IPC2.peptide.svr19 7.613
Protein with the highest isoelectric point:
>sp|P30029|HBSAG_HPBDC Large envelope protein OS=Duck hepatitis B virus (strain China) OX=31510 GN=S PE=3 SV=2
MM1 pKa = 7.45 PQPLKK6 pKa = 10.84 QSLDD10 pKa = 3.26 QSKK13 pKa = 8.61 WLRR16 pKa = 11.84 EE17 pKa = 4.17 AEE19 pKa = 3.91 KK20 pKa = 10.53 HH21 pKa = 6.08 LRR23 pKa = 11.84 EE24 pKa = 4.61 LEE26 pKa = 4.05 NLVDD30 pKa = 4.46 SNLEE34 pKa = 3.97 EE35 pKa = 4.51 EE36 pKa = 4.37 KK37 pKa = 10.94 LKK39 pKa = 10.52 PQLSMGEE46 pKa = 4.42 DD47 pKa = 3.39 VQSPGKK53 pKa = 10.64 GEE55 pKa = 3.85 PLHH58 pKa = 6.29 PNVRR62 pKa = 11.84 APLSHH67 pKa = 6.3 VVRR70 pKa = 11.84 AATIDD75 pKa = 3.69 LPRR78 pKa = 11.84 LGNKK82 pKa = 9.66 LPAKK86 pKa = 9.73 HH87 pKa = 6.56 HH88 pKa = 6.15 LGKK91 pKa = 10.87 LSGLYY96 pKa = 9.38 QMKK99 pKa = 9.56 GCTFNPEE106 pKa = 3.58 WKK108 pKa = 10.59 VPDD111 pKa = 3.45 ISDD114 pKa = 3.37 THH116 pKa = 7.4 FDD118 pKa = 3.61 LQVLNEE124 pKa = 4.19 CPSRR128 pKa = 11.84 NWKK131 pKa = 9.93 YY132 pKa = 8.85 LTPAKK137 pKa = 9.91 FWPKK141 pKa = 9.85 SISYY145 pKa = 9.65 FPVQAGVKK153 pKa = 9.77 AKK155 pKa = 10.91 YY156 pKa = 8.9 PDD158 pKa = 3.27 NVMQHH163 pKa = 5.44 EE164 pKa = 4.89 SIVGKK169 pKa = 10.43 YY170 pKa = 7.4 LTRR173 pKa = 11.84 LYY175 pKa = 10.58 EE176 pKa = 4.03 AGILYY181 pKa = 10.13 KK182 pKa = 10.39 RR183 pKa = 11.84 ISKK186 pKa = 10.27 HH187 pKa = 5.45 LVTFKK192 pKa = 10.35 GQPYY196 pKa = 9.18 KK197 pKa = 10.21 WEE199 pKa = 3.78 HH200 pKa = 5.49 QYY202 pKa = 11.02 LVKK205 pKa = 10.35 QHH207 pKa = 6.21 QIPDD211 pKa = 3.37 GATTRR216 pKa = 11.84 KK217 pKa = 9.71 INGRR221 pKa = 11.84 AEE223 pKa = 3.56 NRR225 pKa = 11.84 RR226 pKa = 11.84 RR227 pKa = 11.84 GDD229 pKa = 3.32 PAKK232 pKa = 10.62 SISRR236 pKa = 11.84 PHH238 pKa = 6.52 DD239 pKa = 3.65 PKK241 pKa = 10.68 RR242 pKa = 11.84 GCNMVRR248 pKa = 11.84 QISNNRR254 pKa = 11.84 SSIRR258 pKa = 11.84 PCANNGGDD266 pKa = 3.25 KK267 pKa = 10.65 HH268 pKa = 7.52 SPTTRR273 pKa = 11.84 RR274 pKa = 11.84 LACWGGKK281 pKa = 9.07 ASRR284 pKa = 11.84 INQSYY289 pKa = 10.53 SSRR292 pKa = 11.84 DD293 pKa = 2.9 SSAPVDD299 pKa = 3.24 ARR301 pKa = 11.84 GRR303 pKa = 11.84 SEE305 pKa = 4.09 SAGSLSSLSGRR316 pKa = 11.84 KK317 pKa = 8.68 AAGNHH322 pKa = 4.8 HH323 pKa = 7.23 HH324 pKa = 6.47 STLINPSVEE333 pKa = 3.78 TTARR337 pKa = 11.84 GRR339 pKa = 11.84 STPGEE344 pKa = 4.08 QISARR349 pKa = 11.84 DD350 pKa = 3.6 TSALPEE356 pKa = 4.18 SRR358 pKa = 11.84 ASRR361 pKa = 11.84 ACNKK365 pKa = 10.09 DD366 pKa = 3.19 SSIKK370 pKa = 10.36 EE371 pKa = 3.82 EE372 pKa = 4.19 NVWYY376 pKa = 10.64 LRR378 pKa = 11.84 GNTSWPNRR386 pKa = 11.84 ITGKK390 pKa = 10.57 LFLVDD395 pKa = 3.43 KK396 pKa = 10.57 NSRR399 pKa = 11.84 NTTEE403 pKa = 3.6 ARR405 pKa = 11.84 LVVDD409 pKa = 5.36 FSQFSKK415 pKa = 10.78 GKK417 pKa = 9.03 NAMRR421 pKa = 11.84 FPRR424 pKa = 11.84 YY425 pKa = 8.48 WSPNLSTLRR434 pKa = 11.84 RR435 pKa = 11.84 ILPVGMPRR443 pKa = 11.84 ISLDD447 pKa = 3.21 LSQAFYY453 pKa = 10.9 HH454 pKa = 6.6 LPLNPASSSRR464 pKa = 11.84 LAVSDD469 pKa = 3.74 GQRR472 pKa = 11.84 VYY474 pKa = 11.07 YY475 pKa = 9.93 FRR477 pKa = 11.84 KK478 pKa = 9.65 APMGVGLSPFLLHH491 pKa = 7.37 LFTTALGSEE500 pKa = 3.97 IARR503 pKa = 11.84 RR504 pKa = 11.84 FNVWTFTYY512 pKa = 9.89 MDD514 pKa = 5.26 DD515 pKa = 5.74 FLLCHH520 pKa = 6.51 PNARR524 pKa = 11.84 HH525 pKa = 6.01 LNSISHH531 pKa = 6.54 AVCTFLQEE539 pKa = 3.68 LGIRR543 pKa = 11.84 INFDD547 pKa = 2.79 KK548 pKa = 8.48 TTPSPVTEE556 pKa = 3.96 IRR558 pKa = 11.84 FLGYY562 pKa = 10.63 QIDD565 pKa = 3.83 QKK567 pKa = 11.0 FMKK570 pKa = 10.02 IEE572 pKa = 3.68 EE573 pKa = 4.43 DD574 pKa = 2.69 RR575 pKa = 11.84 WKK577 pKa = 10.73 EE578 pKa = 3.45 LRR580 pKa = 11.84 TVIKK584 pKa = 9.91 KK585 pKa = 10.07 IKK587 pKa = 9.61 VGAWYY592 pKa = 9.21 DD593 pKa = 3.34 WKK595 pKa = 10.9 CIQRR599 pKa = 11.84 FVGHH603 pKa = 6.96 LNFVLPFTKK612 pKa = 10.62 GNLEE616 pKa = 4.0 MLKK619 pKa = 10.47 PMYY622 pKa = 10.43 AAITNKK628 pKa = 9.7 VNFSFSSAYY637 pKa = 8.34 RR638 pKa = 11.84 TLLYY642 pKa = 10.37 KK643 pKa = 10.16 LTMGVCKK650 pKa = 10.21 LAIKK654 pKa = 9.68 PKK656 pKa = 10.46 SSVPLPRR663 pKa = 11.84 VATDD667 pKa = 3.36 ATPTHH672 pKa = 6.4 GAISHH677 pKa = 5.63 ITGGSAVFAFSKK689 pKa = 10.69 VRR691 pKa = 11.84 DD692 pKa = 3.38 IHH694 pKa = 7.3 IQEE697 pKa = 4.6 LLMVCLAKK705 pKa = 11.01 LMIKK709 pKa = 9.55 PRR711 pKa = 11.84 CILTDD716 pKa = 3.45 STFVCHH722 pKa = 6.96 KK723 pKa = 10.27 RR724 pKa = 11.84 YY725 pKa = 8.06 QTLPWHH731 pKa = 6.55 FAMLAKK737 pKa = 10.32 QLLSPMQLYY746 pKa = 8.02 FVPSKK751 pKa = 10.83 YY752 pKa = 10.6 NPADD756 pKa = 3.51 GPSRR760 pKa = 11.84 HH761 pKa = 6.27 KK762 pKa = 10.97 PPDD765 pKa = 3.32 WTALTYY771 pKa = 10.44 TPLSKK776 pKa = 10.42 AIYY779 pKa = 9.36 IPHH782 pKa = 7.04 RR783 pKa = 11.84 LCGTT787 pKa = 3.88
Molecular weight: 89.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.182
IPC2_protein 9.355
IPC_protein 9.355
Toseland 10.116
ProMoST 9.75
Dawson 10.292
Bjellqvist 9.955
Wikipedia 10.438
Rodwell 10.76
Grimsley 10.35
Solomon 10.306
Lehninger 10.277
Nozaki 10.145
DTASelect 9.94
Thurlkill 10.16
EMBOSS 10.511
Sillero 10.218
Patrickios 10.335
IPC_peptide 10.321
IPC2_peptide 8.682
IPC2.peptide.svr19 8.318
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
1
5
1850
167
787
370.0
41.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.919 ± 0.26
1.351 ± 0.245
4.108 ± 0.392
4.324 ± 0.595
3.622 ± 0.426
5.784 ± 0.559
2.865 ± 0.529
5.892 ± 0.52
6.054 ± 0.645
11.243 ± 1.602
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.838 ± 0.257
3.081 ± 0.729
7.135 ± 0.565
3.892 ± 0.541
6.973 ± 1.181
8.703 ± 0.448
6.27 ± 0.373
4.486 ± 0.389
2.486 ± 0.362
2.973 ± 0.318
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here