Listeria grayi DSM 20601

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria; Listeria grayi

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7UUA2|D7UUA2_LISGR TrpR family protein YerC/YecD OS=Listeria grayi DSM 20601 OX=525367 GN=HMPREF0556_10077 PE=4 SV=1
MM1 pKa = 7.59IKK3 pKa = 10.02LKK5 pKa = 11.01KK6 pKa = 8.72MVIMLAKK13 pKa = 10.57VMIVYY18 pKa = 10.8ASMTGNTEE26 pKa = 3.92EE27 pKa = 4.33IADD30 pKa = 3.65ILGEE34 pKa = 4.09EE35 pKa = 4.34LEE37 pKa = 4.55KK38 pKa = 11.18YY39 pKa = 10.33DD40 pKa = 3.73IEE42 pKa = 4.81VEE44 pKa = 3.91IEE46 pKa = 3.91EE47 pKa = 5.19CISVTPEE54 pKa = 4.29DD55 pKa = 3.72LLEE58 pKa = 4.41YY59 pKa = 10.64DD60 pKa = 3.71GALIGGYY67 pKa = 8.69TYY69 pKa = 11.29DD70 pKa = 5.36DD71 pKa = 3.82GQLPDD76 pKa = 4.05EE77 pKa = 4.84FVDD80 pKa = 5.19FYY82 pKa = 11.56EE83 pKa = 5.81DD84 pKa = 3.28MADD87 pKa = 3.22VDD89 pKa = 4.22FSGMVCASFGSGDD102 pKa = 3.27TFYY105 pKa = 11.39DD106 pKa = 4.14EE107 pKa = 4.93FCLTVDD113 pKa = 4.72LVEE116 pKa = 4.45TRR118 pKa = 11.84LKK120 pKa = 10.31EE121 pKa = 3.85QGATVPVAGFKK132 pKa = 10.6VDD134 pKa = 4.9LDD136 pKa = 3.89PDD138 pKa = 3.68EE139 pKa = 6.77DD140 pKa = 4.01DD141 pKa = 3.46VAKK144 pKa = 10.84AEE146 pKa = 4.63EE147 pKa = 4.24YY148 pKa = 11.21AKK150 pKa = 10.11TYY152 pKa = 11.44ANALLGNAA160 pKa = 4.67

Molecular weight: 17.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7UV25|D7UV25_LISGR Membrane protein insertase YidC OS=Listeria grayi DSM 20601 OX=525367 GN=spoIIIJ PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.29QPSKK9 pKa = 9.59RR10 pKa = 11.84KK11 pKa = 9.09RR12 pKa = 11.84KK13 pKa = 8.78KK14 pKa = 8.32VHH16 pKa = 5.59GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.18NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.73GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2621

0

2621

778444

37

2962

297.0

33.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.09 ± 0.05

0.573 ± 0.012

5.173 ± 0.035

7.219 ± 0.062

4.474 ± 0.044

6.627 ± 0.048

1.992 ± 0.025

7.848 ± 0.046

7.322 ± 0.06

9.662 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.555 ± 0.025

4.24 ± 0.034

3.486 ± 0.026

3.948 ± 0.035

3.968 ± 0.034

5.676 ± 0.032

5.824 ± 0.037

6.806 ± 0.047

0.936 ± 0.017

3.583 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski