Mycobacterium phage EagleEye

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0LN40|W0LN40_9CAUD Uncharacterized protein OS=Mycobacterium phage EagleEye OX=1429759 GN=87 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.22PVKK6 pKa = 10.66EE7 pKa = 4.28FTEE10 pKa = 4.33VWSDD14 pKa = 2.58SWLADD19 pKa = 3.15QVGPMLTCTEE29 pKa = 4.26AEE31 pKa = 3.99ALAALMLGVGKK42 pKa = 9.85PEE44 pKa = 4.92VADD47 pKa = 3.49AWIKK51 pKa = 10.65SHH53 pKa = 6.47ATSDD57 pKa = 3.69DD58 pKa = 4.0CGDD61 pKa = 3.5MHH63 pKa = 7.32CSCNYY68 pKa = 9.48CRR70 pKa = 11.84EE71 pKa = 4.13

Molecular weight: 7.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0LJ59|W0LJ59_9CAUD Uncharacterized protein OS=Mycobacterium phage EagleEye OX=1429759 GN=41 PE=4 SV=1
MM1 pKa = 8.16SEE3 pKa = 3.89EE4 pKa = 4.15DD5 pKa = 3.66SRR7 pKa = 11.84TVSAKK12 pKa = 10.35HH13 pKa = 4.78RR14 pKa = 11.84MCRR17 pKa = 11.84VFQHH21 pKa = 5.31AWEE24 pKa = 4.24YY25 pKa = 7.67TTVKK29 pKa = 10.36RR30 pKa = 11.84DD31 pKa = 3.19GANYY35 pKa = 10.01LQGLACIRR43 pKa = 11.84CGTEE47 pKa = 3.01RR48 pKa = 11.84FMKK51 pKa = 9.96IDD53 pKa = 3.32ARR55 pKa = 11.84TGEE58 pKa = 4.38AGGSKK63 pKa = 10.18YY64 pKa = 10.54KK65 pKa = 10.92YY66 pKa = 10.04ADD68 pKa = 3.62GYY70 pKa = 10.21LFKK73 pKa = 11.09GGGALTVSEE82 pKa = 4.4RR83 pKa = 11.84AALRR87 pKa = 11.84LIEE90 pKa = 4.29VSGHH94 pKa = 4.75LPRR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84KK101 pKa = 9.42SS102 pKa = 3.14

Molecular weight: 11.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

16406

34

1020

172.7

19.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.612 ± 0.316

0.926 ± 0.128

6.607 ± 0.145

6.821 ± 0.277

3.316 ± 0.138

8.204 ± 0.477

1.963 ± 0.168

5.016 ± 0.183

4.968 ± 0.252

7.887 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.134

3.255 ± 0.178

5.48 ± 0.234

3.401 ± 0.191

6.431 ± 0.289

5.925 ± 0.174

5.9 ± 0.186

7.217 ± 0.238

1.969 ± 0.125

2.7 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski