Egretta garzetta (Little egret)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia;

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13489 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091J560|A0A091J560_EGRGA EF-hand domain-containing family member B (Fragment) OS=Egretta garzetta OX=188379 GN=Z169_16260 PE=4 SV=1
LL1 pKa = 6.59YY2 pKa = 10.38VSRR5 pKa = 11.84LSWLEE10 pKa = 3.53NSGVGFSLDD19 pKa = 3.46YY20 pKa = 9.24PTISLHH26 pKa = 6.36AVSRR30 pKa = 11.84DD31 pKa = 3.07LNAYY35 pKa = 7.9PWEE38 pKa = 4.19HH39 pKa = 6.86LYY41 pKa = 11.74VMVNAKK47 pKa = 10.31FEE49 pKa = 4.15EE50 pKa = 4.78EE51 pKa = 4.13EE52 pKa = 4.22TKK54 pKa = 10.32EE55 pKa = 4.09APVAEE60 pKa = 4.6GEE62 pKa = 4.22EE63 pKa = 4.56EE64 pKa = 4.13EE65 pKa = 6.07DD66 pKa = 4.02SDD68 pKa = 6.23DD69 pKa = 3.89DD70 pKa = 4.15VEE72 pKa = 5.15PIAEE76 pKa = 4.09FRR78 pKa = 11.84FVPSDD83 pKa = 3.36KK84 pKa = 10.88SALEE88 pKa = 3.9AMFSAMCEE96 pKa = 4.27CQALHH101 pKa = 7.41PDD103 pKa = 4.21PDD105 pKa = 5.77DD106 pKa = 4.05EE107 pKa = 7.61DD108 pKa = 4.46SDD110 pKa = 3.76NDD112 pKa = 3.97YY113 pKa = 11.37EE114 pKa = 4.59GEE116 pKa = 4.16EE117 pKa = 4.41YY118 pKa = 10.56DD119 pKa = 4.4VEE121 pKa = 4.23AHH123 pKa = 6.13EE124 pKa = 5.43LEE126 pKa = 4.54QGDD129 pKa = 3.63IPTFYY134 pKa = 10.0TYY136 pKa = 11.09EE137 pKa = 4.17EE138 pKa = 4.62GLSHH142 pKa = 6.9LTAEE146 pKa = 4.71GQATLEE152 pKa = 4.11RR153 pKa = 11.84LEE155 pKa = 4.74GMLAQSVSSQYY166 pKa = 11.66NMAGVRR172 pKa = 11.84TEE174 pKa = 3.7DD175 pKa = 3.58SIRR178 pKa = 11.84EE179 pKa = 4.1FEE181 pKa = 5.37DD182 pKa = 3.48GMEE185 pKa = 3.92VDD187 pKa = 3.78IAPVVAGQFEE197 pKa = 4.42DD198 pKa = 5.26AEE200 pKa = 4.32VDD202 pKa = 3.59HH203 pKa = 7.08

Molecular weight: 22.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091JNA5|A0A091JNA5_EGRGA Nucleosome assembly protein 1-like 1 (Fragment) OS=Egretta garzetta OX=188379 GN=Z169_14282 PE=3 SV=1
TT1 pKa = 6.88HH2 pKa = 6.78AQARR6 pKa = 11.84THH8 pKa = 5.63GQTRR12 pKa = 11.84THH14 pKa = 5.63AHH16 pKa = 6.73AGTHH20 pKa = 4.51GHH22 pKa = 5.84TRR24 pKa = 11.84THH26 pKa = 5.17GHH28 pKa = 5.68TRR30 pKa = 11.84THH32 pKa = 5.17GHH34 pKa = 5.68TRR36 pKa = 11.84THH38 pKa = 5.1GHH40 pKa = 5.63TRR42 pKa = 11.84THH44 pKa = 5.57AHH46 pKa = 6.78AGTHH50 pKa = 4.51GHH52 pKa = 5.74TWTHH56 pKa = 4.48GHH58 pKa = 5.7TWTHH62 pKa = 4.48TRR64 pKa = 11.84TWTHH68 pKa = 5.48ACTRR72 pKa = 11.84RR73 pKa = 11.84HH74 pKa = 3.99AHH76 pKa = 6.01ARR78 pKa = 11.84RR79 pKa = 11.84HH80 pKa = 4.22AHH82 pKa = 6.02ARR84 pKa = 11.84THH86 pKa = 4.59GHH88 pKa = 6.25ARR90 pKa = 11.84THH92 pKa = 5.41GHH94 pKa = 5.88TKK96 pKa = 8.13THH98 pKa = 5.28AHH100 pKa = 5.65TWTHH104 pKa = 4.6AHH106 pKa = 5.83TRR108 pKa = 11.84THH110 pKa = 5.75ARR112 pKa = 11.84TWRR115 pKa = 11.84HH116 pKa = 3.14AHH118 pKa = 5.41ARR120 pKa = 11.84TRR122 pKa = 11.84AHH124 pKa = 6.63ARR126 pKa = 11.84THH128 pKa = 5.15VHH130 pKa = 5.29TQTHH134 pKa = 5.91AHH136 pKa = 5.51TQTHH140 pKa = 6.88ACTQRR145 pKa = 11.84HH146 pKa = 4.67

Molecular weight: 16.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13489

0

13489

5606078

31

8762

415.6

46.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.416 ± 0.019

2.204 ± 0.018

5.05 ± 0.015

7.188 ± 0.028

3.986 ± 0.015

5.843 ± 0.026

2.534 ± 0.011

4.986 ± 0.015

6.528 ± 0.028

9.796 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.25 ± 0.009

4.163 ± 0.017

5.216 ± 0.026

4.627 ± 0.02

5.314 ± 0.016

8.118 ± 0.026

5.32 ± 0.017

6.297 ± 0.02

1.2 ± 0.008

2.963 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski