Pomacea canaliculata (Golden apple snail)
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21377 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T7PYU5|A0A2T7PYU5_POMCA Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_01194 PE=4 SV=1
MM1 pKa = 8.19 DD2 pKa = 5.07 GLRR5 pKa = 11.84 QAGHH9 pKa = 5.87 NWGSPHH15 pKa = 7.62 DD16 pKa = 3.88 PTALGEE22 pKa = 4.21 CAPNDD27 pKa = 3.95 TSGGRR32 pKa = 11.84 YY33 pKa = 8.97 IMYY36 pKa = 9.51 PSLSQGNQPNNFRR49 pKa = 11.84 FSPCSIDD56 pKa = 3.63 YY57 pKa = 10.3 ISPVLRR63 pKa = 11.84 DD64 pKa = 3.42 ASQEE68 pKa = 4.04 CFIEE72 pKa = 4.33 SAQDD76 pKa = 3.58 EE77 pKa = 4.65 IHH79 pKa = 6.79 CGNGVVDD86 pKa = 4.53 IRR88 pKa = 11.84 EE89 pKa = 4.1 EE90 pKa = 4.2 CDD92 pKa = 3.07 AGPDD96 pKa = 3.66 GLEE99 pKa = 4.64 GKK101 pKa = 9.55 DD102 pKa = 3.46 KK103 pKa = 10.71 CCSSICLLRR112 pKa = 11.84 EE113 pKa = 3.75 NARR116 pKa = 11.84 CRR118 pKa = 11.84 QFCLLLLKK126 pKa = 10.68 GVLDD130 pKa = 6.09 DD131 pKa = 6.94 DD132 pKa = 6.56 DD133 pKa = 7.6 DD134 pKa = 7.66 DD135 pKa = 7.66 DD136 pKa = 7.69 DD137 pKa = 7.65 DD138 pKa = 7.65 DD139 pKa = 7.65 DD140 pKa = 7.65 DD141 pKa = 7.65 DD142 pKa = 7.65 DD143 pKa = 7.65 DD144 pKa = 7.68 DD145 pKa = 7.67 DD146 pKa = 7.63 DD147 pKa = 7.61 DD148 pKa = 7.17 DD149 pKa = 5.09 DD150 pKa = 4.23
Molecular weight: 16.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.674
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.478
ProMoST 3.872
Dawson 3.732
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.541
Grimsley 3.376
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.151
Thurlkill 3.541
EMBOSS 3.706
Sillero 3.846
Patrickios 1.163
IPC_peptide 3.719
IPC2_peptide 3.808
IPC2.peptide.svr19 3.77
Protein with the highest isoelectric point:
>tr|A0A2T7PKI8|A0A2T7PKI8_POMCA t-SNARE coiled-coil homology domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_05182 PE=3 SV=1
MM1 pKa = 7.29 PRR3 pKa = 11.84 NVKK6 pKa = 10.31 DD7 pKa = 3.46 HH8 pKa = 6.54 GCPIIVSSSFTANTGTLTLQAFLRR32 pKa = 11.84 SIKK35 pKa = 10.45 NPEE38 pKa = 3.64 SAADD42 pKa = 3.5 INQGTNSSDD51 pKa = 2.74 IYY53 pKa = 10.94 SVVRR57 pKa = 11.84 PRR59 pKa = 11.84 RR60 pKa = 11.84 GAPARR65 pKa = 11.84 VFSQTTTSNKK75 pKa = 6.87 QTTAALRR82 pKa = 11.84 RR83 pKa = 11.84 ILTTTTKK90 pKa = 7.74 TTHH93 pKa = 5.87 RR94 pKa = 11.84 TNATLKK100 pKa = 9.83 QATATVYY107 pKa = 8.62 RR108 pKa = 11.84 TTTAKK113 pKa = 10.28 PSRR116 pKa = 11.84 LGVTTRR122 pKa = 11.84 PTTKK126 pKa = 7.43 TTRR129 pKa = 11.84 RR130 pKa = 11.84 GIATSQTTKK139 pKa = 7.86 TTWRR143 pKa = 11.84 GITTRR148 pKa = 11.84 PTTKK152 pKa = 8.13 TTRR155 pKa = 11.84 LGVTTRR161 pKa = 11.84 PTTKK165 pKa = 7.61 TTRR168 pKa = 11.84 IGVTTRR174 pKa = 11.84 PTTRR178 pKa = 11.84 TTRR181 pKa = 11.84 IGVTTRR187 pKa = 11.84 PATRR191 pKa = 11.84 TTRR194 pKa = 11.84 LGVTTKK200 pKa = 8.1 TTTKK204 pKa = 6.06 TTRR207 pKa = 11.84 IGVTTRR213 pKa = 11.84 PTTKK217 pKa = 10.66 ANATLKK223 pKa = 8.91 PTTKK227 pKa = 9.07 TVQRR231 pKa = 11.84 ITTAKK236 pKa = 5.46 TTRR239 pKa = 11.84 LGVTTRR245 pKa = 11.84 PTTRR249 pKa = 11.84 TTRR252 pKa = 11.84 LGVTTRR258 pKa = 11.84 PTTRR262 pKa = 11.84 TTRR265 pKa = 11.84 LGVTTKK271 pKa = 8.1 TTTKK275 pKa = 6.06 TTRR278 pKa = 11.84 IGVTTKK284 pKa = 9.14 PTTKK288 pKa = 7.49 TTRR291 pKa = 11.84 IGVTTRR297 pKa = 11.84 PTTRR301 pKa = 11.84 TTRR304 pKa = 11.84 LGVTTRR310 pKa = 11.84 PTTRR314 pKa = 11.84 TTRR317 pKa = 11.84 LGITTRR323 pKa = 11.84 PTTKK327 pKa = 10.67 ANATLKK333 pKa = 8.91 PTTKK337 pKa = 9.07 TVQRR341 pKa = 11.84 ITTAKK346 pKa = 5.46 TTRR349 pKa = 11.84 LGVTTRR355 pKa = 11.84 PTTKK359 pKa = 8.15 TTRR362 pKa = 11.84 LGITTRR368 pKa = 11.84 PTTRR372 pKa = 11.84 TTRR375 pKa = 11.84 LGITTRR381 pKa = 11.84 PTTRR385 pKa = 11.84 TTRR388 pKa = 11.84 LGITTRR394 pKa = 11.84 PTTRR398 pKa = 11.84 TTRR401 pKa = 11.84 LGITTRR407 pKa = 11.84 PTTRR411 pKa = 11.84 TTRR414 pKa = 11.84 LGITTRR420 pKa = 11.84 PTTRR424 pKa = 11.84 TTRR427 pKa = 11.84 LGVTTRR433 pKa = 11.84 PTTRR437 pKa = 11.84 TTRR440 pKa = 11.84 LGVTTKK446 pKa = 8.1 TTTKK450 pKa = 6.06 TTRR453 pKa = 11.84 IGVTTRR459 pKa = 11.84 PTTRR463 pKa = 11.84 TTRR466 pKa = 11.84 LGVTTRR472 pKa = 11.84 PTTRR476 pKa = 11.84 TTRR479 pKa = 11.84 LGITTRR485 pKa = 11.84 QTTKK489 pKa = 11.09 ANATLKK495 pKa = 8.91 PTTKK499 pKa = 9.07 TVQRR503 pKa = 11.84 ITTAKK508 pKa = 5.46 TTRR511 pKa = 11.84 LGVTTRR517 pKa = 11.84 PTIRR521 pKa = 11.84 TTRR524 pKa = 11.84 LGVTTRR530 pKa = 11.84 PTTRR534 pKa = 11.84 TTRR537 pKa = 11.84 LGITTRR543 pKa = 11.84 PTTRR547 pKa = 11.84 TTRR550 pKa = 11.84 IRR552 pKa = 11.84 VTTRR556 pKa = 11.84 PTTRR560 pKa = 11.84 TTRR563 pKa = 11.84 LGITTRR569 pKa = 11.84 PTTRR573 pKa = 11.84 TTRR576 pKa = 11.84 LGITTRR582 pKa = 11.84 PTTRR586 pKa = 11.84 TTRR589 pKa = 11.84 LGITTRR595 pKa = 11.84 PTTKK599 pKa = 8.17 TTRR602 pKa = 11.84 LGITTRR608 pKa = 11.84 PTTRR612 pKa = 11.84 TTRR615 pKa = 11.84 LGITTRR621 pKa = 11.84 PTTRR625 pKa = 11.84 TTRR628 pKa = 11.84 LGITTRR634 pKa = 11.84 PTTRR638 pKa = 11.84 TTRR641 pKa = 11.84 LGVTTRR647 pKa = 11.84 PTTRR651 pKa = 11.84 TTRR654 pKa = 11.84 LGITTKK660 pKa = 9.26 PTTRR664 pKa = 11.84 TTRR667 pKa = 11.84 LGVTTRR673 pKa = 11.84 PTTRR677 pKa = 11.84 TTRR680 pKa = 11.84 LGVTTRR686 pKa = 11.84 PTIRR690 pKa = 11.84 TTRR693 pKa = 11.84 LGITTRR699 pKa = 11.84 PTTKK703 pKa = 8.17 TTRR706 pKa = 11.84 LGITTRR712 pKa = 11.84 PTTKK716 pKa = 8.17 TTRR719 pKa = 11.84 LGITTRR725 pKa = 11.84 PTTKK729 pKa = 10.22 TNATLKK735 pKa = 8.77 PTTKK739 pKa = 9.22 TVQSITTAKK748 pKa = 6.0 TTRR751 pKa = 11.84 LGVTTRR757 pKa = 11.84 PTTRR761 pKa = 11.84 TTRR764 pKa = 11.84 LGVTTRR770 pKa = 11.84 PTTRR774 pKa = 11.84 TTRR777 pKa = 11.84 LGVTTRR783 pKa = 11.84 PTTRR787 pKa = 11.84 TTRR790 pKa = 11.84 LGVTTRR796 pKa = 11.84 PTTRR800 pKa = 11.84 TTRR803 pKa = 11.84 LGVITRR809 pKa = 11.84 PTTRR813 pKa = 11.84 TTRR816 pKa = 11.84 LGVTTRR822 pKa = 11.84 PSTRR826 pKa = 11.84 TTRR829 pKa = 11.84 LGVTTRR835 pKa = 11.84 PTTRR839 pKa = 11.84 TTRR842 pKa = 11.84 LGITTRR848 pKa = 11.84 PTTKK852 pKa = 10.22 TNATLKK858 pKa = 8.77 PTTKK862 pKa = 9.22 TVQSITTAKK871 pKa = 6.0 TTRR874 pKa = 11.84 LGVTTRR880 pKa = 11.84 PTTRR884 pKa = 11.84 TTRR887 pKa = 11.84 IGVTTRR893 pKa = 11.84 AITKK897 pKa = 6.41 TTLPRR902 pKa = 11.84 VSARR906 pKa = 11.84 PTAKK910 pKa = 10.03 TNTTLKK916 pKa = 8.99 PTTKK920 pKa = 9.06 TVQSITTAKK929 pKa = 6.0 TTRR932 pKa = 11.84 LGVTTRR938 pKa = 11.84 PTTRR942 pKa = 11.84 TTRR945 pKa = 11.84 IGVTTRR951 pKa = 11.84 AITKK955 pKa = 6.41 TTLPRR960 pKa = 11.84 VSARR964 pKa = 11.84 PTAKK968 pKa = 10.07 TNTTLKK974 pKa = 9.56 STTKK978 pKa = 8.93 TVQSITTAKK987 pKa = 5.58 TTRR990 pKa = 11.84 PRR992 pKa = 11.84 VSARR996 pKa = 11.84 TTTKK1000 pKa = 7.6 TTKK1003 pKa = 9.62 LTTQKK1008 pKa = 7.25 TTRR1011 pKa = 11.84 TRR1013 pKa = 11.84 VTTRR1017 pKa = 11.84 PTTKK1021 pKa = 8.92 STRR1024 pKa = 11.84 PRR1026 pKa = 11.84 VTSRR1030 pKa = 11.84 PTTNTTRR1037 pKa = 11.84 PTTQKK1042 pKa = 6.6 TTRR1045 pKa = 11.84 TRR1047 pKa = 11.84 VTTRR1051 pKa = 11.84 RR1052 pKa = 11.84 TTKK1055 pKa = 6.09 TTRR1058 pKa = 11.84 PRR1060 pKa = 11.84 VTSRR1064 pKa = 11.84 RR1065 pKa = 11.84 TTKK1068 pKa = 7.26 TTRR1071 pKa = 11.84 PTTQKK1076 pKa = 8.94 STRR1079 pKa = 11.84 VTIRR1083 pKa = 11.84 RR1084 pKa = 11.84 TSKK1087 pKa = 6.56 TTRR1090 pKa = 11.84 PTTQKK1095 pKa = 8.86 STRR1098 pKa = 11.84 VTTRR1102 pKa = 11.84 RR1103 pKa = 11.84 TTKK1106 pKa = 6.72 TTRR1109 pKa = 11.84 PTTQKK1114 pKa = 6.21 TTRR1117 pKa = 11.84 VTTRR1121 pKa = 11.84 RR1122 pKa = 11.84 TTKK1125 pKa = 6.09 TTRR1128 pKa = 11.84 PRR1130 pKa = 11.84 VTSRR1134 pKa = 11.84 RR1135 pKa = 11.84 TTKK1138 pKa = 7.26 TTRR1141 pKa = 11.84 PTTQKK1146 pKa = 6.6 TTRR1149 pKa = 11.84 TRR1151 pKa = 11.84 VTARR1155 pKa = 11.84 PTTKK1159 pKa = 8.05 TTRR1162 pKa = 11.84 PTTHH1166 pKa = 6.6 KK1167 pKa = 10.42 AARR1170 pKa = 11.84 TRR1172 pKa = 11.84 GTTRR1176 pKa = 11.84 PTTKK1180 pKa = 7.47 TTRR1183 pKa = 11.84 RR1184 pKa = 11.84 GVNARR1189 pKa = 11.84 ITTPKK1194 pKa = 10.38 VKK1196 pKa = 9.95 TPAQTAAPLLSEE1208 pKa = 4.81 RR1209 pKa = 11.84 STEE1212 pKa = 4.28 GKK1214 pKa = 9.5 SLRR1217 pKa = 11.84 SDD1219 pKa = 2.97 RR1220 pKa = 11.84 GGEE1223 pKa = 3.72 LRR1225 pKa = 11.84 AVVGVTVGVLGLVIIAALIYY1245 pKa = 9.16 FLRR1248 pKa = 11.84 KK1249 pKa = 9.28 RR1250 pKa = 11.84 RR1251 pKa = 11.84 IDD1253 pKa = 3.54 ANVGDD1258 pKa = 4.95 IYY1260 pKa = 11.54 NVDD1263 pKa = 3.51 LL1264 pKa = 4.89
Molecular weight: 139.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.359
IPC_protein 12.896
Toseland 13.056
ProMoST 13.554
Dawson 13.056
Bjellqvist 13.056
Wikipedia 13.539
Rodwell 12.705
Grimsley 13.1
Solomon 13.554
Lehninger 13.466
Nozaki 13.056
DTASelect 13.056
Thurlkill 13.056
EMBOSS 13.554
Sillero 13.056
Patrickios 12.398
IPC_peptide 13.568
IPC2_peptide 12.544
IPC2.peptide.svr19 9.231
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21377
0
21377
10621572
52
10929
496.9
55.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.776 ± 0.017
2.322 ± 0.019
5.598 ± 0.017
6.27 ± 0.024
3.624 ± 0.012
6.188 ± 0.032
2.698 ± 0.011
4.476 ± 0.013
5.375 ± 0.022
9.064 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.337 ± 0.007
4.025 ± 0.013
5.235 ± 0.025
4.643 ± 0.018
5.718 ± 0.017
8.696 ± 0.028
6.227 ± 0.02
6.821 ± 0.018
1.165 ± 0.008
2.741 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here