Capybara microvirus Cap1_SP_108
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4E9|A0A4P8W4E9_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_108 OX=2584806 PE=4 SV=1
MM1 pKa = 7.66 NIYY4 pKa = 10.35 SIYY7 pKa = 11.29 DD8 pKa = 3.2 MVARR12 pKa = 11.84 EE13 pKa = 3.97 FSSPFLAKK21 pKa = 10.22 NDD23 pKa = 3.58 GVAMRR28 pKa = 11.84 MFGNTCRR35 pKa = 11.84 SEE37 pKa = 5.12 RR38 pKa = 11.84 IVNPNEE44 pKa = 3.69 LEE46 pKa = 4.17 LYY48 pKa = 10.62 CIGKK52 pKa = 9.32 FLGEE56 pKa = 4.2 NLDD59 pKa = 4.19 GCIEE63 pKa = 4.04 PCKK66 pKa = 10.39 PMIIRR71 pKa = 11.84 VSEE74 pKa = 4.09 SDD76 pKa = 3.33 VV77 pKa = 3.24
Molecular weight: 8.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.709
IPC2_protein 4.914
IPC_protein 4.685
Toseland 4.533
ProMoST 4.774
Dawson 4.609
Bjellqvist 4.762
Wikipedia 4.469
Rodwell 4.52
Grimsley 4.444
Solomon 4.596
Lehninger 4.558
Nozaki 4.724
DTASelect 4.825
Thurlkill 4.533
EMBOSS 4.482
Sillero 4.774
Patrickios 3.503
IPC_peptide 4.609
IPC2_peptide 4.774
IPC2.peptide.svr19 4.776
Protein with the highest isoelectric point:
>tr|A0A4P8W3Y6|A0A4P8W3Y6_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_108 OX=2584806 PE=3 SV=1
MM1 pKa = 8.01 PCGQCVNCRR10 pKa = 11.84 KK11 pKa = 10.22 RR12 pKa = 11.84 IRR14 pKa = 11.84 SQWTGRR20 pKa = 11.84 MMMEE24 pKa = 4.18 NLTHH28 pKa = 6.72 KK29 pKa = 10.62 ASVFITLSYY38 pKa = 11.4 DD39 pKa = 3.64 DD40 pKa = 3.74 VHH42 pKa = 8.58 LPIIKK47 pKa = 9.84 INEE50 pKa = 4.05 RR51 pKa = 11.84 SFTSYY56 pKa = 11.09 DD57 pKa = 3.29 SEE59 pKa = 4.06 LTYY62 pKa = 10.55 RR63 pKa = 11.84 DD64 pKa = 4.16 CCCLDD69 pKa = 3.27 AHH71 pKa = 6.82 NILHH75 pKa = 6.75 PVRR78 pKa = 11.84 LPIFSSTDD86 pKa = 3.02 TSDD89 pKa = 5.22 GIISDD94 pKa = 4.64 FASCRR99 pKa = 11.84 PNTFLTDD106 pKa = 3.59 YY107 pKa = 11.04 SNSPCVDD114 pKa = 5.04 EE115 pKa = 5.42 IPVLAPLQPRR125 pKa = 11.84 DD126 pKa = 3.4 TQLFLKK132 pKa = 10.36 RR133 pKa = 11.84 LRR135 pKa = 11.84 INLKK139 pKa = 10.03 RR140 pKa = 11.84 QFGLSDD146 pKa = 3.16 KK147 pKa = 10.79 LRR149 pKa = 11.84 YY150 pKa = 8.59 FLCGEE155 pKa = 4.09 YY156 pKa = 10.76 GEE158 pKa = 4.6 EE159 pKa = 3.75 RR160 pKa = 11.84 GRR162 pKa = 11.84 PHH164 pKa = 7.17 YY165 pKa = 10.94 HH166 pKa = 6.41 MILFGLDD173 pKa = 3.49 VSPSSYY179 pKa = 10.47 RR180 pKa = 11.84 CRR182 pKa = 11.84 DD183 pKa = 3.27 SNSYY187 pKa = 10.32 SKK189 pKa = 10.91 AYY191 pKa = 10.52 ALDD194 pKa = 3.57 SVFRR198 pKa = 11.84 CIQDD202 pKa = 2.65 SWSLGRR208 pKa = 11.84 IEE210 pKa = 4.98 CEE212 pKa = 3.55 PVTEE216 pKa = 4.39 SNLRR220 pKa = 11.84 YY221 pKa = 8.13 VAKK224 pKa = 9.28 YY225 pKa = 4.67 TTKK228 pKa = 10.55 TSMDD232 pKa = 3.81 SAPSIEE238 pKa = 3.6 WDD240 pKa = 3.67 YY241 pKa = 11.55 YY242 pKa = 10.32 IRR244 pKa = 11.84 QCLNYY249 pKa = 9.94 LKK251 pKa = 10.3 HH252 pKa = 6.03 NCPMKK257 pKa = 10.62 CDD259 pKa = 3.73 SFCFDD264 pKa = 2.86 SAKK267 pKa = 10.48 RR268 pKa = 11.84 FLRR271 pKa = 11.84 SLRR274 pKa = 11.84 DD275 pKa = 3.36 FDD277 pKa = 4.15 IYY279 pKa = 10.62 PYY281 pKa = 9.8 GQKK284 pKa = 10.12 PFHH287 pKa = 6.27 RR288 pKa = 11.84 QSLGLGYY295 pKa = 10.72 DD296 pKa = 3.73 FFYY299 pKa = 11.45 KK300 pKa = 10.74 NIDD303 pKa = 3.71 SFAHH307 pKa = 6.02 KK308 pKa = 9.95 PYY310 pKa = 9.25 FTSASSKK317 pKa = 9.93 IRR319 pKa = 11.84 YY320 pKa = 7.88 SFPKK324 pKa = 10.19 CFYY327 pKa = 10.73 DD328 pKa = 3.49 RR329 pKa = 11.84 YY330 pKa = 10.71 KK331 pKa = 10.16 RR332 pKa = 11.84 TTFRR336 pKa = 11.84 LYY338 pKa = 10.8 QNSLLQKK345 pKa = 10.08 YY346 pKa = 9.78 DD347 pKa = 3.33 FEE349 pKa = 5.98 SYY351 pKa = 7.82 VHH353 pKa = 6.81 CCNNMWSSLYY363 pKa = 10.0 EE364 pKa = 4.31 SKK366 pKa = 10.92 GLNYY370 pKa = 10.34 RR371 pKa = 11.84 PIFPSAKK378 pKa = 9.92 IKK380 pKa = 10.87 DD381 pKa = 4.0 LFLKK385 pKa = 10.51 SRR387 pKa = 11.84 IYY389 pKa = 11.02 GSFSNDD395 pKa = 3.11 YY396 pKa = 9.56 FPSFRR401 pKa = 11.84 SPVPSYY407 pKa = 10.96 KK408 pKa = 10.08 KK409 pKa = 9.65 WFEE412 pKa = 4.28 SYY414 pKa = 10.56 SKK416 pKa = 10.91 HH417 pKa = 5.71 NLWHH421 pKa = 6.44 KK422 pKa = 10.25 MPVLAHH428 pKa = 5.99 SSVFVMFKK436 pKa = 10.51 PPVSPPSYY444 pKa = 11.35 NEE446 pKa = 3.97 FCSLTYY452 pKa = 10.71 KK453 pKa = 10.11 HH454 pKa = 6.6 LKK456 pKa = 9.15 RR457 pKa = 4.93
Molecular weight: 53.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.465
IPC2_protein 8.375
IPC_protein 8.185
Toseland 8.097
ProMoST 8.756
Dawson 8.931
Bjellqvist 9.121
Wikipedia 8.975
Rodwell 8.975
Grimsley 8.185
Solomon 8.975
Lehninger 8.96
Nozaki 9.224
DTASelect 8.902
Thurlkill 8.946
EMBOSS 9.077
Sillero 9.209
Patrickios 3.668
IPC_peptide 8.975
IPC2_peptide 8.185
IPC2.peptide.svr19 8.168
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1669
77
581
278.2
31.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.752 ± 1.077
2.037 ± 0.865
6.291 ± 0.314
6.471 ± 1.102
6.111 ± 0.691
6.052 ± 1.202
2.397 ± 0.273
5.213 ± 0.456
5.632 ± 1.149
7.549 ± 0.743
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.636 ± 0.445
6.111 ± 0.66
4.254 ± 1.026
2.217 ± 0.367
5.872 ± 0.566
9.706 ± 1.393
3.954 ± 0.93
5.992 ± 0.997
1.138 ± 0.222
4.614 ± 0.958
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here