Gordonia phage Mariokart

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sourvirus; unclassified Sourvirus

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9A0R1|A0A7G9A0R1_9CAUD Uncharacterized protein OS=Gordonia phage Mariokart OX=2759455 GN=73 PE=4 SV=1
MM1 pKa = 8.17PDD3 pKa = 3.52TAPKK7 pKa = 9.92FAPTAKK13 pKa = 10.25RR14 pKa = 11.84GTEE17 pKa = 4.01LLRR20 pKa = 11.84HH21 pKa = 5.31VMDD24 pKa = 5.44IIEE27 pKa = 4.74AEE29 pKa = 4.13AEE31 pKa = 3.69AAANVTEE38 pKa = 3.7QDD40 pKa = 3.15TRR42 pKa = 11.84VGHH45 pKa = 6.53WNQGDD50 pKa = 4.38WIRR53 pKa = 11.84LRR55 pKa = 11.84ADD57 pKa = 3.5EE58 pKa = 4.65LAEE61 pKa = 4.63DD62 pKa = 4.2LDD64 pKa = 4.05LRR66 pKa = 11.84ALAVEE71 pKa = 4.05AAGAKK76 pKa = 10.34SFDD79 pKa = 4.12EE80 pKa = 5.07LGTDD84 pKa = 3.6PGHH87 pKa = 6.99LGVSVPINPEE97 pKa = 3.53TGLLDD102 pKa = 3.74LCGTACCFAGHH113 pKa = 7.37ASLQVGDD120 pKa = 4.25YY121 pKa = 10.59PVLTTNAATLYY132 pKa = 10.79NITAPGADD140 pKa = 3.66QIMEE144 pKa = 4.15MGLNLVIPIEE154 pKa = 4.38TVAEE158 pKa = 4.2SGGAPLTTLPYY169 pKa = 9.96EE170 pKa = 4.51SVSGRR175 pKa = 11.84AQEE178 pKa = 4.25LLGLSGSDD186 pKa = 3.76ADD188 pKa = 4.23TLFDD192 pKa = 4.98GSNTLADD199 pKa = 3.81LRR201 pKa = 11.84EE202 pKa = 4.03LVARR206 pKa = 11.84LEE208 pKa = 4.25RR209 pKa = 11.84GEE211 pKa = 4.93EE212 pKa = 3.79IDD214 pKa = 4.09NLKK217 pKa = 8.97CTACDD222 pKa = 3.69EE223 pKa = 4.66YY224 pKa = 10.82PWNCNGGEE232 pKa = 4.16VCDD235 pKa = 3.87TCNEE239 pKa = 4.26HH240 pKa = 7.42EE241 pKa = 4.48DD242 pKa = 3.52HH243 pKa = 7.09CLCDD247 pKa = 3.14ICEE250 pKa = 4.17VCDD253 pKa = 4.17RR254 pKa = 11.84NVDD257 pKa = 5.2AGNCWCEE264 pKa = 3.99TCDD267 pKa = 3.45EE268 pKa = 4.58CGSRR272 pKa = 11.84VIEE275 pKa = 4.21TTQDD279 pKa = 2.96EE280 pKa = 4.57DD281 pKa = 4.42TLNDD285 pKa = 3.95LCDD288 pKa = 4.13DD289 pKa = 3.75CHH291 pKa = 7.6DD292 pKa = 3.65RR293 pKa = 11.84AEE295 pKa = 4.28AEE297 pKa = 3.95RR298 pKa = 11.84EE299 pKa = 3.81EE300 pKa = 4.27RR301 pKa = 11.84RR302 pKa = 11.84VAEE305 pKa = 4.12EE306 pKa = 4.55ADD308 pKa = 3.81DD309 pKa = 5.46EE310 pKa = 4.65EE311 pKa = 7.12DD312 pKa = 4.73DD313 pKa = 5.76DD314 pKa = 4.61EE315 pKa = 4.68

Molecular weight: 34.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9A0Q9|A0A7G9A0Q9_9CAUD Uncharacterized protein OS=Gordonia phage Mariokart OX=2759455 GN=71 PE=4 SV=1
MM1 pKa = 7.64ASNRR5 pKa = 11.84EE6 pKa = 4.06VKK8 pKa = 10.66DD9 pKa = 3.89LIRR12 pKa = 11.84EE13 pKa = 4.09IEE15 pKa = 4.03QAGGEE20 pKa = 3.99IRR22 pKa = 11.84RR23 pKa = 11.84SKK25 pKa = 10.78RR26 pKa = 11.84NAHH29 pKa = 6.12LKK31 pKa = 10.57VYY33 pKa = 10.89LNGNLIGTISATPSDD48 pKa = 3.19WRR50 pKa = 11.84SIKK53 pKa = 10.36NSRR56 pKa = 11.84AQLRR60 pKa = 11.84RR61 pKa = 11.84NGLDD65 pKa = 2.73II66 pKa = 5.12

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

19050

46

1761

229.5

24.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.446 ± 0.287

0.94 ± 0.174

7.181 ± 0.276

5.853 ± 0.289

2.373 ± 0.153

8.635 ± 0.379

1.822 ± 0.146

4.43 ± 0.247

2.499 ± 0.185

7.948 ± 0.193

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.13

2.583 ± 0.185

5.517 ± 0.234

3.129 ± 0.155

6.756 ± 0.334

5.696 ± 0.2

7.186 ± 0.235

8.147 ± 0.202

2.01 ± 0.088

2.383 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski