Caldiarchaeum subterraneum

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Thaumarchaeota incertae sedis; Candidatus Caldarchaeum

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6N358|E6N358_CALS0 Thioredoxin reductase OS=Caldiarchaeum subterraneum OX=311458 GN=HGMM_F22F09C14 PE=4 SV=1
MM1 pKa = 7.31GLEE4 pKa = 4.07PTTPDD9 pKa = 3.69LEE11 pKa = 4.56GRR13 pKa = 11.84CAIHH17 pKa = 5.97SAPRR21 pKa = 11.84ALTVDD26 pKa = 4.08EE27 pKa = 4.52KK28 pKa = 10.98RR29 pKa = 11.84CGVFEE34 pKa = 4.81LSLSVGPSDD43 pKa = 3.7EE44 pKa = 4.68SFFEE48 pKa = 4.1EE49 pKa = 5.82GEE51 pKa = 4.24DD52 pKa = 3.71EE53 pKa = 4.63EE54 pKa = 5.25EE55 pKa = 4.23ADD57 pKa = 4.22SYY59 pKa = 11.45EE60 pKa = 3.95GDD62 pKa = 3.72YY63 pKa = 11.72EE64 pKa = 4.32EE65 pKa = 5.25ACEE68 pKa = 4.55DD69 pKa = 3.45

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6N330|E6N330_CALS0 Translation initiation factor eIF-2B OS=Caldiarchaeum subterraneum OX=311458 GN=CSUB_C1164 PE=3 SV=1
MM1 pKa = 8.1SFEE4 pKa = 4.77DD5 pKa = 3.75LNNRR9 pKa = 11.84IISCKK14 pKa = 9.82LCPRR18 pKa = 11.84LVKK21 pKa = 10.58YY22 pKa = 10.55RR23 pKa = 11.84EE24 pKa = 4.05EE25 pKa = 4.17VAKK28 pKa = 10.83NPPKK32 pKa = 10.48RR33 pKa = 11.84FRR35 pKa = 11.84GQTYY39 pKa = 7.14WAKK42 pKa = 9.86PLPGFGDD49 pKa = 3.19VGARR53 pKa = 11.84VLVLGLAPAAHH64 pKa = 6.74GGNRR68 pKa = 11.84TGRR71 pKa = 11.84MFTGDD76 pKa = 3.28SSGDD80 pKa = 3.41TLVRR84 pKa = 11.84ALHH87 pKa = 6.19RR88 pKa = 11.84AGFANMGRR96 pKa = 11.84SISINDD102 pKa = 3.65GLVLKK107 pKa = 10.29DD108 pKa = 3.44VYY110 pKa = 9.39ITAVVRR116 pKa = 11.84CAPPDD121 pKa = 3.59NKK123 pKa = 9.96PAKK126 pKa = 10.18QEE128 pKa = 4.01VEE130 pKa = 3.75NCLPYY135 pKa = 10.64LIEE138 pKa = 4.16EE139 pKa = 4.3LRR141 pKa = 11.84MLEE144 pKa = 4.03NVVVVVALGRR154 pKa = 11.84FAFEE158 pKa = 3.89TFFRR162 pKa = 11.84LMRR165 pKa = 11.84RR166 pKa = 11.84MNAYY170 pKa = 9.58SGRR173 pKa = 11.84IPRR176 pKa = 11.84FRR178 pKa = 11.84HH179 pKa = 4.37GAVYY183 pKa = 10.56RR184 pKa = 11.84LNGSFNGKK192 pKa = 8.76PLPTVIASYY201 pKa = 10.59HH202 pKa = 6.51PSRR205 pKa = 11.84QNTSTGRR212 pKa = 11.84LTQRR216 pKa = 11.84MIDD219 pKa = 3.88SVFHH223 pKa = 6.63RR224 pKa = 11.84ARR226 pKa = 11.84AIAGLL231 pKa = 3.76

Molecular weight: 25.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2154

0

2154

631940

43

2586

293.4

32.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.465 ± 0.055

0.86 ± 0.019

4.514 ± 0.034

7.067 ± 0.06

3.963 ± 0.033

7.561 ± 0.047

1.806 ± 0.02

5.91 ± 0.04

5.463 ± 0.046

10.576 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.457 ± 0.025

2.981 ± 0.029

4.631 ± 0.034

2.496 ± 0.027

6.441 ± 0.041

5.879 ± 0.04

5.225 ± 0.046

9.188 ± 0.047

1.106 ± 0.02

3.411 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski