Human papillomavirus 39
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P24838|VL1_HPV39 Major capsid protein L1 OS=Human papillomavirus 39 OX=10588 GN=L1 PE=3 SV=1
MM1 pKa = 7.92 RR2 pKa = 11.84 GPKK5 pKa = 8.48 PTLQEE10 pKa = 4.0 IVLDD14 pKa = 3.79 LCPYY18 pKa = 10.95 NEE20 pKa = 4.34 IQPVDD25 pKa = 4.22 LVCHH29 pKa = 5.44 EE30 pKa = 4.22 QLGEE34 pKa = 4.16 SEE36 pKa = 5.31 DD37 pKa = 4.4 EE38 pKa = 3.96 IDD40 pKa = 4.66 EE41 pKa = 4.61 PDD43 pKa = 3.34 HH44 pKa = 6.8 AVNHH48 pKa = 4.82 QHH50 pKa = 6.25 QLLARR55 pKa = 11.84 RR56 pKa = 11.84 DD57 pKa = 3.48 EE58 pKa = 4.45 PQRR61 pKa = 11.84 HH62 pKa = 5.95 TIQCSCCKK70 pKa = 10.36 CNNTLQLVVEE80 pKa = 4.76 ASRR83 pKa = 11.84 DD84 pKa = 3.63 TLRR87 pKa = 11.84 QLQQLFMDD95 pKa = 4.27 SLGFVCPWCATANQQ109 pKa = 3.65
Molecular weight: 12.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.483
IPC2_protein 4.762
IPC_protein 4.647
Toseland 4.533
ProMoST 4.774
Dawson 4.622
Bjellqvist 4.762
Wikipedia 4.507
Rodwell 4.52
Grimsley 4.457
Solomon 4.622
Lehninger 4.571
Nozaki 4.749
DTASelect 4.902
Thurlkill 4.546
EMBOSS 4.533
Sillero 4.8
Patrickios 2.041
IPC_peptide 4.622
IPC2_peptide 4.787
IPC2.peptide.svr19 4.717
Protein with the highest isoelectric point:
>sp|P24829|VE1_HPV39 Replication protein E1 OS=Human papillomavirus 39 OX=10588 GN=E1 PE=3 SV=1
MM1 pKa = 7.76 AIWKK5 pKa = 7.87 WKK7 pKa = 6.48 QLKK10 pKa = 9.33 WSTSDD15 pKa = 3.03 GSVPTTEE22 pKa = 4.35 LTTEE26 pKa = 4.63 LSNTTATHH34 pKa = 5.33 STATTPCTQKK44 pKa = 9.88 TIPPPSRR51 pKa = 11.84 KK52 pKa = 9.32 RR53 pKa = 11.84 PRR55 pKa = 11.84 QCAVTEE61 pKa = 3.97 PTEE64 pKa = 4.12 PDD66 pKa = 4.07 GVSLDD71 pKa = 3.77 HH72 pKa = 6.99 LNNPLHH78 pKa = 6.67 SNSTGHH84 pKa = 4.91 NTRR87 pKa = 11.84 RR88 pKa = 11.84 YY89 pKa = 8.91 LSCGNTTPIIHH100 pKa = 7.1 LKK102 pKa = 9.86 GDD104 pKa = 3.68 KK105 pKa = 10.75 NGLKK109 pKa = 10.14 CLRR112 pKa = 11.84 YY113 pKa = 9.56 RR114 pKa = 11.84 LQKK117 pKa = 10.66 YY118 pKa = 7.59 DD119 pKa = 3.66 TLFEE123 pKa = 5.07 NISCTWHH130 pKa = 6.68 WIRR133 pKa = 11.84 GKK135 pKa = 8.24 GTKK138 pKa = 9.47 NAGILTVTYY147 pKa = 8.48 ATEE150 pKa = 3.96 SQRR153 pKa = 11.84 QKK155 pKa = 11.07 FLDD158 pKa = 3.7 TVKK161 pKa = 10.22 IPSSVHH167 pKa = 4.93 VSLGYY172 pKa = 7.7 MTLL175 pKa = 3.52
Molecular weight: 19.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.13
IPC2_protein 8.902
IPC_protein 8.829
Toseland 9.604
ProMoST 9.326
Dawson 9.838
Bjellqvist 9.589
Wikipedia 9.984
Rodwell 10.233
Grimsley 9.882
Solomon 9.882
Lehninger 9.853
Nozaki 9.794
DTASelect 9.516
Thurlkill 9.706
EMBOSS 10.028
Sillero 9.809
Patrickios 7.571
IPC_peptide 9.882
IPC2_peptide 8.39
IPC2.peptide.svr19 7.936
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
2597
72
647
288.6
32.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.468 ± 0.524
2.926 ± 0.706
5.93 ± 0.699
4.505 ± 0.551
3.466 ± 0.563
5.583 ± 0.707
2.349 ± 0.361
5.006 ± 0.405
5.044 ± 0.632
8.048 ± 0.807
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.041 ± 0.32
4.428 ± 0.397
6.007 ± 1.04
4.351 ± 0.558
5.16 ± 0.562
7.624 ± 0.932
9.665 ± 1.04
6.469 ± 0.545
1.656 ± 0.317
4.274 ± 0.476
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here