Amino acid dipepetide frequency for Clostridium sp. CAG:510

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.72AlaAla: 7.72 ± 0.15	1.088AlaCys: 1.088 ± 0.04	5.212AlaAsp: 5.212 ± 0.096	6.575AlaGlu: 6.575 ± 0.112	3.298AlaPhe: 3.298 ± 0.068	6.712AlaGly: 6.712 ± 0.114	1.119AlaHis: 1.119 ± 0.037	4.648AlaIle: 4.648 ± 0.083	4.744AlaLys: 4.744 ± 0.085	6.882AlaLeu: 6.882 ± 0.101	2.327AlaMet: 2.327 ± 0.053	2.463AlaAsn: 2.463 ± 0.063	2.108AlaPro: 2.108 ± 0.06	2.237AlaGln: 2.237 ± 0.046	2.648AlaArg: 2.648 ± 0.058	3.841AlaSer: 3.841 ± 0.08	3.275AlaThr: 3.275 ± 0.075	6.596AlaVal: 6.596 ± 0.099	0.587AlaTrp: 0.587 ± 0.031	3.136AlaTyr: 3.136 ± 0.056	0.001AlaXaa: 0.001 ± 0.001
Cys	0.976CysAla: 0.976 ± 0.035	0.238CysCys: 0.238 ± 0.019	0.79CysAsp: 0.79 ± 0.033	0.901CysGlu: 0.901 ± 0.033	0.637CysPhe: 0.637 ± 0.028	1.535CysGly: 1.535 ± 0.048	0.309CysHis: 0.309 ± 0.019	1.086CysIle: 1.086 ± 0.04	0.813CysLys: 0.813 ± 0.032	1.253CysLeu: 1.253 ± 0.047	0.519CysMet: 0.519 ± 0.026	0.603CysAsn: 0.603 ± 0.032	0.593CysPro: 0.593 ± 0.031	0.321CysGln: 0.321 ± 0.022	0.776CysArg: 0.776 ± 0.032	0.817CysSer: 0.817 ± 0.027	0.729CysThr: 0.729 ± 0.033	1.071CysVal: 1.071 ± 0.037	0.111CysTrp: 0.111 ± 0.013	0.657CysTyr: 0.657 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	4.664AspAla: 4.664 ± 0.072	0.795AspCys: 0.795 ± 0.035	2.797AspAsp: 2.797 ± 0.072	4.531AspGlu: 4.531 ± 0.091	2.802AspPhe: 2.802 ± 0.057	4.639AspGly: 4.639 ± 0.097	0.685AspHis: 0.685 ± 0.03	4.53AspIle: 4.53 ± 0.076	3.889AspLys: 3.889 ± 0.073	4.297AspLeu: 4.297 ± 0.076	1.98AspMet: 1.98 ± 0.042	2.358AspAsn: 2.358 ± 0.055	1.432AspPro: 1.432 ± 0.048	0.747AspGln: 0.747 ± 0.03	2.606AspArg: 2.606 ± 0.081	2.899AspSer: 2.899 ± 0.069	3.417AspThr: 3.417 ± 0.071	4.072AspVal: 4.072 ± 0.074	0.602AspTrp: 0.602 ± 0.031	2.919AspTyr: 2.919 ± 0.08	0.0AspXaa: 0.0 ± 0.0
Glu	5.973GluAla: 5.973 ± 0.096	0.813GluCys: 0.813 ± 0.032	4.621GluAsp: 4.621 ± 0.096	8.627GluGlu: 8.627 ± 0.133	2.463GluPhe: 2.463 ± 0.05	4.659GluGly: 4.659 ± 0.08	1.255GluHis: 1.255 ± 0.041	5.493GluIle: 5.493 ± 0.096	7.593GluLys: 7.593 ± 0.118	6.567GluLeu: 6.567 ± 0.097	2.458GluMet: 2.458 ± 0.066	4.611GluAsn: 4.611 ± 0.076	1.906GluPro: 1.906 ± 0.051	2.861GluGln: 2.861 ± 0.066	3.223GluArg: 3.223 ± 0.077	3.464GluSer: 3.464 ± 0.065	3.942GluThr: 3.942 ± 0.072	4.655GluVal: 4.655 ± 0.077	0.694GluTrp: 0.694 ± 0.034	3.292GluTyr: 3.292 ± 0.069	0.0GluXaa: 0.0 ± 0.0
Phe	3.247PheAla: 3.247 ± 0.069	0.761PheCys: 0.761 ± 0.028	2.496PheAsp: 2.496 ± 0.057	2.424PheGlu: 2.424 ± 0.062	2.029PhePhe: 2.029 ± 0.055	2.869PheGly: 2.869 ± 0.073	0.787PheHis: 0.787 ± 0.031	2.649PheIle: 2.649 ± 0.078	1.952PheLys: 1.952 ± 0.058	4.367PheLeu: 4.367 ± 0.088	1.24PheMet: 1.24 ± 0.035	1.383PheAsn: 1.383 ± 0.045	1.466PhePro: 1.466 ± 0.04	1.115PheGln: 1.115 ± 0.04	1.72PheArg: 1.72 ± 0.045	2.935PheSer: 2.935 ± 0.075	2.679PheThr: 2.679 ± 0.061	2.811PheVal: 2.811 ± 0.063	0.403PheTrp: 0.403 ± 0.026	1.983PheTyr: 1.983 ± 0.053	0.0PheXaa: 0.0 ± 0.0
Gly	4.858GlyAla: 4.858 ± 0.081	1.287GlyCys: 1.287 ± 0.049	3.61GlyAsp: 3.61 ± 0.072	5.135GlyGlu: 5.135 ± 0.084	3.13GlyPhe: 3.13 ± 0.064	4.695GlyGly: 4.695 ± 0.107	1.19GlyHis: 1.19 ± 0.038	6.153GlyIle: 6.153 ± 0.109	5.983GlyLys: 5.983 ± 0.093	5.711GlyLeu: 5.711 ± 0.09	2.693GlyMet: 2.693 ± 0.053	3.4GlyAsn: 3.4 ± 0.073	1.2GlyPro: 1.2 ± 0.041	2.05GlyGln: 2.05 ± 0.05	3.316GlyArg: 3.316 ± 0.071	4.168GlySer: 4.168 ± 0.079	4.41GlyThr: 4.41 ± 0.094	4.801GlyVal: 4.801 ± 0.07	0.625GlyTrp: 0.625 ± 0.035	3.329GlyTyr: 3.329 ± 0.061	0.001GlyXaa: 0.001 ± 0.001
His	1.124HisAla: 1.124 ± 0.038	0.266HisCys: 0.266 ± 0.017	0.828HisAsp: 0.828 ± 0.035	0.969HisGlu: 0.969 ± 0.04	0.844HisPhe: 0.844 ± 0.032	1.079HisGly: 1.079 ± 0.039	0.383HisHis: 0.383 ± 0.035	1.307HisIle: 1.307 ± 0.04	0.991HisLys: 0.991 ± 0.035	1.386HisLeu: 1.386 ± 0.041	0.616HisMet: 0.616 ± 0.029	0.684HisAsn: 0.684 ± 0.025	0.792HisPro: 0.792 ± 0.032	0.388HisGln: 0.388 ± 0.023	0.756HisArg: 0.756 ± 0.03	0.862HisSer: 0.862 ± 0.036	0.971HisThr: 0.971 ± 0.034	1.13HisVal: 1.13 ± 0.035	0.142HisTrp: 0.142 ± 0.013	0.761HisTyr: 0.761 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.803IleAla: 5.803 ± 0.077	1.287IleCys: 1.287 ± 0.043	3.573IleAsp: 3.573 ± 0.075	4.476IleGlu: 4.476 ± 0.087	2.802IlePhe: 2.802 ± 0.063	5.015IleGly: 5.015 ± 0.099	1.181IleHis: 1.181 ± 0.043	4.629IleIle: 4.629 ± 0.088	4.17IleLys: 4.17 ± 0.081	6.867IleLeu: 6.867 ± 0.1	1.886IleMet: 1.886 ± 0.051	2.656IleAsn: 2.656 ± 0.066	3.012IlePro: 3.012 ± 0.065	1.932IleGln: 1.932 ± 0.052	3.416IleArg: 3.416 ± 0.058	4.744IleSer: 4.744 ± 0.08	4.265IleThr: 4.265 ± 0.084	4.804IleVal: 4.804 ± 0.083	0.607IleTrp: 0.607 ± 0.032	2.849IleTyr: 2.849 ± 0.062	0.0IleXaa: 0.0 ± 0.0
Lys	5.279LysAla: 5.279 ± 0.089	0.732LysCys: 0.732 ± 0.031	4.261LysAsp: 4.261 ± 0.078	7.622LysGlu: 7.622 ± 0.113	1.892LysPhe: 1.892 ± 0.056	4.374LysGly: 4.374 ± 0.07	0.949LysHis: 0.949 ± 0.034	4.972LysIle: 4.972 ± 0.079	6.798LysLys: 6.798 ± 0.109	5.307LysLeu: 5.307 ± 0.085	2.288LysMet: 2.288 ± 0.061	3.77LysAsn: 3.77 ± 0.063	2.083LysPro: 2.083 ± 0.05	2.197LysGln: 2.197 ± 0.054	2.915LysArg: 2.915 ± 0.058	3.156LysSer: 3.156 ± 0.062	3.835LysThr: 3.835 ± 0.064	4.578LysVal: 4.578 ± 0.087	0.662LysTrp: 0.662 ± 0.027	2.779LysTyr: 2.779 ± 0.059	0.0LysXaa: 0.0 ± 0.0
Leu	6.702LeuAla: 6.702 ± 0.104	1.628LeuCys: 1.628 ± 0.042	4.492LeuAsp: 4.492 ± 0.077	5.207LeuGlu: 5.207 ± 0.086	4.097LeuPhe: 4.097 ± 0.094	5.453LeuGly: 5.453 ± 0.1	1.712LeuHis: 1.712 ± 0.055	5.842LeuIle: 5.842 ± 0.111	6.139LeuLys: 6.139 ± 0.103	9.295LeuLeu: 9.295 ± 0.149	2.492LeuMet: 2.492 ± 0.063	3.609LeuAsn: 3.609 ± 0.069	3.672LeuPro: 3.672 ± 0.074	3.323LeuGln: 3.323 ± 0.066	3.716LeuArg: 3.716 ± 0.072	6.562LeuSer: 6.562 ± 0.102	5.745LeuThr: 5.745 ± 0.091	5.102LeuVal: 5.102 ± 0.084	0.802LeuTrp: 0.802 ± 0.036	3.781LeuTyr: 3.781 ± 0.081	0.001LeuXaa: 0.001 ± 0.001
Met	2.514MetAla: 2.514 ± 0.059	0.343MetCys: 0.343 ± 0.02	1.962MetAsp: 1.962 ± 0.042	2.797MetGlu: 2.797 ± 0.062	0.933MetPhe: 0.933 ± 0.035	2.162MetGly: 2.162 ± 0.058	0.537MetHis: 0.537 ± 0.025	1.967MetIle: 1.967 ± 0.052	2.485MetLys: 2.485 ± 0.056	2.714MetLeu: 2.714 ± 0.069	0.933MetMet: 0.933 ± 0.036	1.431MetAsn: 1.431 ± 0.042	1.216MetPro: 1.216 ± 0.037	1.253MetGln: 1.253 ± 0.04	1.202MetArg: 1.202 ± 0.039	1.645MetSer: 1.645 ± 0.049	1.76MetThr: 1.76 ± 0.05	2.097MetVal: 2.097 ± 0.05	0.228MetTrp: 0.228 ± 0.016	0.992MetTyr: 0.992 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	3.284AsnAla: 3.284 ± 0.068	0.596AsnCys: 0.596 ± 0.029	2.011AsnAsp: 2.011 ± 0.054	2.676AsnGlu: 2.676 ± 0.062	1.61AsnPhe: 1.61 ± 0.051	3.806AsnGly: 3.806 ± 0.079	0.728AsnHis: 0.728 ± 0.029	3.284AsnIle: 3.284 ± 0.069	2.6AsnLys: 2.6 ± 0.062	3.81AsnLeu: 3.81 ± 0.078	1.406AsnMet: 1.406 ± 0.044	1.91AsnAsn: 1.91 ± 0.053	1.961AsnPro: 1.961 ± 0.049	1.102AsnGln: 1.102 ± 0.036	2.12AsnArg: 2.12 ± 0.052	2.23AsnSer: 2.23 ± 0.053	2.499AsnThr: 2.499 ± 0.062	2.935AsnVal: 2.935 ± 0.069	0.393AsnTrp: 0.393 ± 0.023	1.886AsnTyr: 1.886 ± 0.057	0.001AsnXaa: 0.001 ± 0.001
Pro	2.846ProAla: 2.846 ± 0.065	0.416ProCys: 0.416 ± 0.028	2.42ProAsp: 2.42 ± 0.06	3.4ProGlu: 3.4 ± 0.071	1.465ProPhe: 1.465 ± 0.048	2.398ProGly: 2.398 ± 0.05	0.542ProHis: 0.542 ± 0.025	1.96ProIle: 1.96 ± 0.063	1.801ProLys: 1.801 ± 0.052	2.722ProLeu: 2.722 ± 0.06	0.88ProMet: 0.88 ± 0.037	1.088ProAsn: 1.088 ± 0.038	0.653ProPro: 0.653 ± 0.027	0.941ProGln: 0.941 ± 0.032	0.916ProArg: 0.916 ± 0.031	1.572ProSer: 1.572 ± 0.047	1.49ProThr: 1.49 ± 0.045	3.058ProVal: 3.058 ± 0.07	0.28ProTrp: 0.28 ± 0.018	1.523ProTyr: 1.523 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	2.263GlnAla: 2.263 ± 0.055	0.309GlnCys: 0.309 ± 0.017	1.587GlnAsp: 1.587 ± 0.044	2.397GlnGlu: 2.397 ± 0.051	1.171GlnPhe: 1.171 ± 0.037	1.809GlnGly: 1.809 ± 0.049	0.4GlnHis: 0.4 ± 0.022	2.37GlnIle: 2.37 ± 0.063	2.688GlnLys: 2.688 ± 0.052	2.271GlnLeu: 2.271 ± 0.06	1.071GlnMet: 1.071 ± 0.039	1.641GlnAsn: 1.641 ± 0.042	0.893GlnPro: 0.893 ± 0.031	1.036GlnGln: 1.036 ± 0.037	1.104GlnArg: 1.104 ± 0.038	1.496GlnSer: 1.496 ± 0.046	1.782GlnThr: 1.782 ± 0.064	1.924GlnVal: 1.924 ± 0.049	0.251GlnTrp: 0.251 ± 0.017	1.257GlnTyr: 1.257 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	2.532ArgAla: 2.532 ± 0.057	0.53ArgCys: 0.53 ± 0.03	2.183ArgAsp: 2.183 ± 0.052	3.93ArgGlu: 3.93 ± 0.086	1.736ArgPhe: 1.736 ± 0.046	2.381ArgGly: 2.381 ± 0.06	0.711ArgHis: 0.711 ± 0.026	3.379ArgIle: 3.379 ± 0.065	3.604ArgLys: 3.604 ± 0.071	3.845ArgLeu: 3.845 ± 0.074	1.55ArgMet: 1.55 ± 0.046	1.971ArgAsn: 1.971 ± 0.045	1.224ArgPro: 1.224 ± 0.049	1.7ArgGln: 1.7 ± 0.075	1.96ArgArg: 1.96 ± 0.051	1.977ArgSer: 1.977 ± 0.052	2.275ArgThr: 2.275 ± 0.054	2.684ArgVal: 2.684 ± 0.058	0.324ArgTrp: 0.324 ± 0.022	1.964ArgTyr: 1.964 ± 0.055	0.001ArgXaa: 0.001 ± 0.001
Ser	4.23SerAla: 4.23 ± 0.08	0.798SerCys: 0.798 ± 0.035	3.093SerAsp: 3.093 ± 0.065	3.672SerGlu: 3.672 ± 0.075	2.735SerPhe: 2.735 ± 0.061	4.793SerGly: 4.793 ± 0.096	0.959SerHis: 0.959 ± 0.031	3.776SerIle: 3.776 ± 0.073	3.027SerLys: 3.027 ± 0.063	5.301SerLeu: 5.301 ± 0.091	1.852SerMet: 1.852 ± 0.048	2.074SerAsn: 2.074 ± 0.047	1.652SerPro: 1.652 ± 0.039	1.525SerGln: 1.525 ± 0.046	2.575SerArg: 2.575 ± 0.052	3.069SerSer: 3.069 ± 0.066	2.856SerThr: 2.856 ± 0.07	4.374SerVal: 4.374 ± 0.072	0.448SerTrp: 0.448 ± 0.024	2.683SerTyr: 2.683 ± 0.067	0.0SerXaa: 0.0 ± 0.0
Thr	4.751ThrAla: 4.751 ± 0.099	0.747ThrCys: 0.747 ± 0.041	3.724ThrAsp: 3.724 ± 0.096	4.733ThrGlu: 4.733 ± 0.084	2.248ThrPhe: 2.248 ± 0.046	5.243ThrGly: 5.243 ± 0.103	0.845ThrHis: 0.845 ± 0.033	3.646ThrIle: 3.646 ± 0.072	2.97ThrLys: 2.97 ± 0.06	5.027ThrLeu: 5.027 ± 0.064	1.482ThrMet: 1.482 ± 0.042	1.934ThrAsn: 1.934 ± 0.049	2.224ThrPro: 2.224 ± 0.058	1.47ThrGln: 1.47 ± 0.046	1.972ThrArg: 1.972 ± 0.053	2.632ThrSer: 2.632 ± 0.055	2.878ThrThr: 2.878 ± 0.066	4.964ThrVal: 4.964 ± 0.113	0.476ThrTrp: 0.476 ± 0.025	2.481ThrTyr: 2.481 ± 0.068	0.0ThrXaa: 0.0 ± 0.0
Val	4.875ValAla: 4.875 ± 0.086	1.281ValCys: 1.281 ± 0.037	3.812ValAsp: 3.812 ± 0.068	4.839ValGlu: 4.839 ± 0.085	3.098ValPhe: 3.098 ± 0.072	4.196ValGly: 4.196 ± 0.078	1.019ValHis: 1.019 ± 0.034	4.871ValIle: 4.871 ± 0.085	4.936ValLys: 4.936 ± 0.083	6.899ValLeu: 6.899 ± 0.105	2.015ValMet: 2.015 ± 0.05	2.967ValAsn: 2.967 ± 0.067	2.56ValPro: 2.56 ± 0.055	1.9ValGln: 1.9 ± 0.045	2.864ValArg: 2.864 ± 0.065	4.686ValSer: 4.686 ± 0.085	4.574ValThr: 4.574 ± 0.097	4.951ValVal: 4.951 ± 0.091	0.62ValTrp: 0.62 ± 0.029	3.079ValTyr: 3.079 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.512TrpAla: 0.512 ± 0.026	0.147TrpCys: 0.147 ± 0.015	0.515TrpAsp: 0.515 ± 0.03	0.677TrpGlu: 0.677 ± 0.031	0.389TrpPhe: 0.389 ± 0.022	0.667TrpGly: 0.667 ± 0.032	0.158TrpHis: 0.158 ± 0.015	0.569TrpIle: 0.569 ± 0.03	0.721TrpLys: 0.721 ± 0.032	0.86TrpLeu: 0.86 ± 0.032	0.309TrpMet: 0.309 ± 0.021	0.456TrpAsn: 0.456 ± 0.025	0.204TrpPro: 0.204 ± 0.018	0.367TrpGln: 0.367 ± 0.022	0.285TrpArg: 0.285 ± 0.018	0.437TrpSer: 0.437 ± 0.023	0.408TrpThr: 0.408 ± 0.022	0.486TrpVal: 0.486 ± 0.022	0.095TrpTrp: 0.095 ± 0.011	0.453TrpTyr: 0.453 ± 0.029	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.063TyrAla: 3.063 ± 0.082	0.672TyrCys: 0.672 ± 0.031	2.672TyrAsp: 2.672 ± 0.067	3.583TyrGlu: 3.583 ± 0.069	1.935TyrPhe: 1.935 ± 0.055	3.259TyrGly: 3.259 ± 0.073	0.809TyrHis: 0.809 ± 0.03	2.843TyrIle: 2.843 ± 0.059	2.544TyrLys: 2.544 ± 0.066	3.99TyrLeu: 3.99 ± 0.081	1.173TyrMet: 1.173 ± 0.04	1.838TyrAsn: 1.838 ± 0.06	1.521TyrPro: 1.521 ± 0.046	1.257TyrGln: 1.257 ± 0.038	2.374TyrArg: 2.374 ± 0.057	2.301TyrSer: 2.301 ± 0.05	2.656TyrThr: 2.656 ± 0.081	2.95TyrVal: 2.95 ± 0.061	0.365TyrTrp: 0.365 ± 0.024	1.995TyrTyr: 1.995 ± 0.068	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.001XaaHis: 0.001 ± 0.001	0.002XaaIle: 0.002 ± 0.002	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.004XaaXaa: 0.004 ± 0.002

Statistics based on 2541 proteins (814344 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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