Amino acid dipepetide frequency for Mycobacterium phage MichelleMyBell

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.513AlaAla: 19.513 ± 2.92
0.877AlaCys: 0.877 ± 0.339
8.112AlaAsp: 8.112 ± 0.919
8.916AlaGlu: 8.916 ± 1.216
3.508AlaPhe: 3.508 ± 0.609
9.428AlaGly: 9.428 ± 1.564
2.339AlaHis: 2.339 ± 0.389
6.285AlaIle: 6.285 ± 0.699
3.362AlaLys: 3.362 ± 0.631
8.624AlaLeu: 8.624 ± 0.823
2.046AlaMet: 2.046 ± 0.481
2.85AlaAsn: 2.85 ± 0.502
7.455AlaPro: 7.455 ± 0.891
4.824AlaGln: 4.824 ± 0.81
8.332AlaArg: 8.332 ± 0.839
6.431AlaSer: 6.431 ± 0.676
7.601AlaThr: 7.601 ± 0.899
7.016AlaVal: 7.016 ± 0.657
1.681AlaTrp: 1.681 ± 0.368
1.973AlaTyr: 1.973 ± 0.354
0.0AlaXaa: 0.0 ± 0.0
Cys
1.389CysAla: 1.389 ± 0.334
0.073CysCys: 0.073 ± 0.073
0.877CysAsp: 0.877 ± 0.278
0.365CysGlu: 0.365 ± 0.154
0.0CysPhe: 0.0 ± 0.0
1.169CysGly: 1.169 ± 0.391
0.073CysHis: 0.073 ± 0.066
0.219CysIle: 0.219 ± 0.123
0.146CysLys: 0.146 ± 0.088
0.804CysLeu: 0.804 ± 0.232
0.146CysMet: 0.146 ± 0.094
0.219CysAsn: 0.219 ± 0.125
0.877CysPro: 0.877 ± 0.344
0.292CysGln: 0.292 ± 0.153
0.731CysArg: 0.731 ± 0.208
0.585CysSer: 0.585 ± 0.2
0.731CysThr: 0.731 ± 0.234
0.731CysVal: 0.731 ± 0.254
0.146CysTrp: 0.146 ± 0.104
0.219CysTyr: 0.219 ± 0.11
0.0CysXaa: 0.0 ± 0.0
Asp
7.162AspAla: 7.162 ± 0.748
0.804AspCys: 0.804 ± 0.192
4.75AspAsp: 4.75 ± 0.596
5.116AspGlu: 5.116 ± 0.783
1.9AspPhe: 1.9 ± 0.388
7.235AspGly: 7.235 ± 0.722
1.535AspHis: 1.535 ± 0.364
2.777AspIle: 2.777 ± 0.503
1.535AspLys: 1.535 ± 0.338
5.701AspLeu: 5.701 ± 0.667
1.681AspMet: 1.681 ± 0.317
2.046AspAsn: 2.046 ± 0.385
4.385AspPro: 4.385 ± 0.646
2.046AspGln: 2.046 ± 0.34
3.873AspArg: 3.873 ± 0.594
3.727AspSer: 3.727 ± 0.473
2.996AspThr: 2.996 ± 0.532
3.947AspVal: 3.947 ± 0.472
1.023AspTrp: 1.023 ± 0.371
1.608AspTyr: 1.608 ± 0.368
0.0AspXaa: 0.0 ± 0.0
Glu
5.847GluAla: 5.847 ± 0.628
0.585GluCys: 0.585 ± 0.189
3.508GluAsp: 3.508 ± 0.495
2.339GluGlu: 2.339 ± 0.5
2.558GluPhe: 2.558 ± 0.474
3.143GluGly: 3.143 ± 0.606
1.096GluHis: 1.096 ± 0.291
3.508GluIle: 3.508 ± 0.505
2.193GluLys: 2.193 ± 0.368
5.554GluLeu: 5.554 ± 0.744
1.535GluMet: 1.535 ± 0.378
1.9GluAsn: 1.9 ± 0.282
2.996GluPro: 2.996 ± 0.561
3.07GluGln: 3.07 ± 0.46
5.262GluArg: 5.262 ± 0.661
2.996GluSer: 2.996 ± 0.432
4.312GluThr: 4.312 ± 0.523
4.604GluVal: 4.604 ± 0.72
1.389GluTrp: 1.389 ± 0.306
1.681GluTyr: 1.681 ± 0.414
0.0GluXaa: 0.0 ± 0.0
Phe
4.02PheAla: 4.02 ± 0.609
0.439PheCys: 0.439 ± 0.212
1.9PheAsp: 1.9 ± 0.421
1.535PheGlu: 1.535 ± 0.375
1.096PhePhe: 1.096 ± 0.27
2.485PheGly: 2.485 ± 0.475
0.658PheHis: 0.658 ± 0.254
1.023PheIle: 1.023 ± 0.297
0.95PheLys: 0.95 ± 0.231
1.9PheLeu: 1.9 ± 0.347
0.512PheMet: 0.512 ± 0.23
0.804PheAsn: 0.804 ± 0.246
1.096PhePro: 1.096 ± 0.267
0.439PheGln: 0.439 ± 0.174
1.608PheArg: 1.608 ± 0.292
1.462PheSer: 1.462 ± 0.294
2.412PheThr: 2.412 ± 0.381
2.631PheVal: 2.631 ± 0.442
0.292PheTrp: 0.292 ± 0.133
0.95PheTyr: 0.95 ± 0.249
0.0PheXaa: 0.0 ± 0.0
Gly
8.405GlyAla: 8.405 ± 1.246
1.096GlyCys: 1.096 ± 0.356
5.481GlyAsp: 5.481 ± 0.619
4.312GlyGlu: 4.312 ± 0.626
2.485GlyPhe: 2.485 ± 0.587
11.766GlyGly: 11.766 ± 2.422
1.242GlyHis: 1.242 ± 0.324
4.458GlyIle: 4.458 ± 0.728
2.046GlyLys: 2.046 ± 0.313
6.943GlyLeu: 6.943 ± 0.772
1.389GlyMet: 1.389 ± 0.285
3.727GlyAsn: 3.727 ± 0.596
5.262GlyPro: 5.262 ± 0.691
3.508GlyGln: 3.508 ± 0.463
6.504GlyArg: 6.504 ± 0.613
5.335GlySer: 5.335 ± 0.856
5.701GlyThr: 5.701 ± 0.565
6.504GlyVal: 6.504 ± 0.655
2.119GlyTrp: 2.119 ± 0.415
2.923GlyTyr: 2.923 ± 0.544
0.0GlyXaa: 0.0 ± 0.0
His
1.462HisAla: 1.462 ± 0.316
0.365HisCys: 0.365 ± 0.175
1.389HisAsp: 1.389 ± 0.251
1.608HisGlu: 1.608 ± 0.46
0.219HisPhe: 0.219 ± 0.114
1.754HisGly: 1.754 ± 0.348
0.731HisHis: 0.731 ± 0.229
0.877HisIle: 0.877 ± 0.299
0.512HisLys: 0.512 ± 0.196
1.608HisLeu: 1.608 ± 0.385
0.219HisMet: 0.219 ± 0.112
0.219HisAsn: 0.219 ± 0.116
1.242HisPro: 1.242 ± 0.33
0.658HisGln: 0.658 ± 0.195
2.046HisArg: 2.046 ± 0.407
0.365HisSer: 0.365 ± 0.165
1.389HisThr: 1.389 ± 0.312
1.608HisVal: 1.608 ± 0.363
0.439HisTrp: 0.439 ± 0.164
0.95HisTyr: 0.95 ± 0.273
0.0HisXaa: 0.0 ± 0.0
Ile
6.139IleAla: 6.139 ± 0.604
0.146IleCys: 0.146 ± 0.108
4.458IleAsp: 4.458 ± 0.635
3.435IleGlu: 3.435 ± 0.44
0.877IlePhe: 0.877 ± 0.266
5.043IleGly: 5.043 ± 0.741
1.023IleHis: 1.023 ± 0.241
1.316IleIle: 1.316 ± 0.322
0.95IleLys: 0.95 ± 0.252
2.85IleLeu: 2.85 ± 0.4
0.439IleMet: 0.439 ± 0.179
2.119IleAsn: 2.119 ± 0.372
2.631IlePro: 2.631 ± 0.442
1.462IleGln: 1.462 ± 0.3
3.654IleArg: 3.654 ± 0.478
2.193IleSer: 2.193 ± 0.355
3.581IleThr: 3.581 ± 0.476
3.143IleVal: 3.143 ± 0.485
0.804IleTrp: 0.804 ± 0.241
0.95IleTyr: 0.95 ± 0.179
0.0IleXaa: 0.0 ± 0.0
Lys
4.385LysAla: 4.385 ± 0.984
0.146LysCys: 0.146 ± 0.099
1.242LysAsp: 1.242 ± 0.25
1.242LysGlu: 1.242 ± 0.286
0.877LysPhe: 0.877 ± 0.203
2.119LysGly: 2.119 ± 0.488
0.365LysHis: 0.365 ± 0.158
1.608LysIle: 1.608 ± 0.303
0.658LysLys: 0.658 ± 0.224
1.608LysLeu: 1.608 ± 0.338
0.658LysMet: 0.658 ± 0.196
0.512LysAsn: 0.512 ± 0.198
2.923LysPro: 2.923 ± 0.416
1.462LysGln: 1.462 ± 0.34
2.412LysArg: 2.412 ± 0.437
1.316LysSer: 1.316 ± 0.313
1.462LysThr: 1.462 ± 0.307
2.193LysVal: 2.193 ± 0.384
0.512LysTrp: 0.512 ± 0.181
0.95LysTyr: 0.95 ± 0.227
0.0LysXaa: 0.0 ± 0.0
Leu
9.939LeuAla: 9.939 ± 1.23
0.512LeuCys: 0.512 ± 0.197
5.262LeuAsp: 5.262 ± 0.618
3.947LeuGlu: 3.947 ± 0.383
2.996LeuPhe: 2.996 ± 0.472
7.381LeuGly: 7.381 ± 0.716
1.608LeuHis: 1.608 ± 0.345
3.654LeuIle: 3.654 ± 0.435
2.631LeuLys: 2.631 ± 0.363
6.139LeuLeu: 6.139 ± 0.702
1.169LeuMet: 1.169 ± 0.367
2.339LeuAsn: 2.339 ± 0.47
3.654LeuPro: 3.654 ± 0.524
2.339LeuGln: 2.339 ± 0.44
5.774LeuArg: 5.774 ± 0.772
3.873LeuSer: 3.873 ± 0.551
5.043LeuThr: 5.043 ± 0.618
5.262LeuVal: 5.262 ± 0.559
1.389LeuTrp: 1.389 ± 0.288
1.316LeuTyr: 1.316 ± 0.27
0.0LeuXaa: 0.0 ± 0.0
Met
2.996MetAla: 2.996 ± 0.511
0.0MetCys: 0.0 ± 0.0
0.95MetAsp: 0.95 ± 0.262
0.512MetGlu: 0.512 ± 0.237
0.877MetPhe: 0.877 ± 0.249
0.585MetGly: 0.585 ± 0.189
0.439MetHis: 0.439 ± 0.169
0.95MetIle: 0.95 ± 0.258
0.804MetLys: 0.804 ± 0.254
1.169MetLeu: 1.169 ± 0.292
0.292MetMet: 0.292 ± 0.13
0.877MetAsn: 0.877 ± 0.271
0.95MetPro: 0.95 ± 0.251
0.512MetGln: 0.512 ± 0.17
1.242MetArg: 1.242 ± 0.272
2.558MetSer: 2.558 ± 0.362
1.973MetThr: 1.973 ± 0.409
0.877MetVal: 0.877 ± 0.207
0.804MetTrp: 0.804 ± 0.289
0.219MetTyr: 0.219 ± 0.114
0.0MetXaa: 0.0 ± 0.0
Asn
3.362AsnAla: 3.362 ± 0.525
0.292AsnCys: 0.292 ± 0.142
1.242AsnAsp: 1.242 ± 0.273
0.804AsnGlu: 0.804 ± 0.22
0.731AsnPhe: 0.731 ± 0.217
4.531AsnGly: 4.531 ± 0.807
0.439AsnHis: 0.439 ± 0.191
2.193AsnIle: 2.193 ± 0.433
0.585AsnLys: 0.585 ± 0.243
2.485AsnLeu: 2.485 ± 0.421
0.365AsnMet: 0.365 ± 0.158
0.877AsnAsn: 0.877 ± 0.273
2.923AsnPro: 2.923 ± 0.388
1.242AsnGln: 1.242 ± 0.317
2.119AsnArg: 2.119 ± 0.428
1.389AsnSer: 1.389 ± 0.33
1.754AsnThr: 1.754 ± 0.355
1.973AsnVal: 1.973 ± 0.349
0.658AsnTrp: 0.658 ± 0.181
0.512AsnTyr: 0.512 ± 0.213
0.0AsnXaa: 0.0 ± 0.0
Pro
8.112ProAla: 8.112 ± 0.82
0.439ProCys: 0.439 ± 0.167
4.677ProAsp: 4.677 ± 0.52
5.408ProGlu: 5.408 ± 0.681
1.9ProPhe: 1.9 ± 0.397
5.701ProGly: 5.701 ± 0.822
1.169ProHis: 1.169 ± 0.329
1.9ProIle: 1.9 ± 0.363
1.242ProLys: 1.242 ± 0.289
4.02ProLeu: 4.02 ± 0.581
1.535ProMet: 1.535 ± 0.408
1.827ProAsn: 1.827 ± 0.384
3.435ProPro: 3.435 ± 0.586
2.119ProGln: 2.119 ± 0.349
2.996ProArg: 2.996 ± 0.349
3.289ProSer: 3.289 ± 0.497
4.093ProThr: 4.093 ± 0.531
3.947ProVal: 3.947 ± 0.581
1.681ProTrp: 1.681 ± 0.388
1.316ProTyr: 1.316 ± 0.419
0.0ProXaa: 0.0 ± 0.0
Gln
5.043GlnAla: 5.043 ± 1.01
0.292GlnCys: 0.292 ± 0.133
2.046GlnAsp: 2.046 ± 0.509
1.462GlnGlu: 1.462 ± 0.326
0.512GlnPhe: 0.512 ± 0.2
1.827GlnGly: 1.827 ± 0.299
1.023GlnHis: 1.023 ± 0.226
2.558GlnIle: 2.558 ± 0.406
1.316GlnLys: 1.316 ± 0.305
4.093GlnLeu: 4.093 ± 0.535
0.585GlnMet: 0.585 ± 0.213
0.365GlnAsn: 0.365 ± 0.143
2.193GlnPro: 2.193 ± 0.428
1.754GlnGln: 1.754 ± 0.544
2.85GlnArg: 2.85 ± 0.561
1.681GlnSer: 1.681 ± 0.356
2.193GlnThr: 2.193 ± 0.378
2.85GlnVal: 2.85 ± 0.38
0.658GlnTrp: 0.658 ± 0.208
1.023GlnTyr: 1.023 ± 0.318
0.0GlnXaa: 0.0 ± 0.0
Arg
6.504ArgAla: 6.504 ± 0.75
1.023ArgCys: 1.023 ± 0.308
4.239ArgAsp: 4.239 ± 0.7
5.116ArgGlu: 5.116 ± 0.922
1.9ArgPhe: 1.9 ± 0.385
5.335ArgGly: 5.335 ± 0.675
1.608ArgHis: 1.608 ± 0.359
3.289ArgIle: 3.289 ± 0.471
2.193ArgLys: 2.193 ± 0.468
5.993ArgLeu: 5.993 ± 0.717
2.193ArgMet: 2.193 ± 0.632
2.119ArgAsn: 2.119 ± 0.327
4.166ArgPro: 4.166 ± 0.558
3.143ArgGln: 3.143 ± 0.62
6.797ArgArg: 6.797 ± 0.978
3.216ArgSer: 3.216 ± 0.498
4.312ArgThr: 4.312 ± 0.743
5.043ArgVal: 5.043 ± 0.597
1.462ArgTrp: 1.462 ± 0.3
1.389ArgTyr: 1.389 ± 0.325
0.0ArgXaa: 0.0 ± 0.0
Ser
6.358SerAla: 6.358 ± 0.91
0.292SerCys: 0.292 ± 0.153
3.947SerAsp: 3.947 ± 0.529
2.339SerGlu: 2.339 ± 0.348
1.535SerPhe: 1.535 ± 0.295
6.066SerGly: 6.066 ± 0.659
1.023SerHis: 1.023 ± 0.343
1.9SerIle: 1.9 ± 0.393
1.754SerLys: 1.754 ± 0.303
3.508SerLeu: 3.508 ± 0.542
2.119SerMet: 2.119 ± 0.299
1.535SerAsn: 1.535 ± 0.422
3.435SerPro: 3.435 ± 0.357
1.973SerGln: 1.973 ± 0.373
3.216SerArg: 3.216 ± 0.484
2.85SerSer: 2.85 ± 0.429
3.216SerThr: 3.216 ± 0.452
3.07SerVal: 3.07 ± 0.486
1.096SerTrp: 1.096 ± 0.273
1.462SerTyr: 1.462 ± 0.25
0.0SerXaa: 0.0 ± 0.0
Thr
8.551ThrAla: 8.551 ± 0.618
0.512ThrCys: 0.512 ± 0.181
4.312ThrAsp: 4.312 ± 0.601
3.362ThrGlu: 3.362 ± 0.519
1.681ThrPhe: 1.681 ± 0.404
6.578ThrGly: 6.578 ± 0.929
0.95ThrHis: 0.95 ± 0.295
3.289ThrIle: 3.289 ± 0.525
1.9ThrLys: 1.9 ± 0.34
5.335ThrLeu: 5.335 ± 0.566
0.95ThrMet: 0.95 ± 0.252
1.681ThrAsn: 1.681 ± 0.296
4.824ThrPro: 4.824 ± 0.618
2.119ThrGln: 2.119 ± 0.401
3.581ThrArg: 3.581 ± 0.589
3.581ThrSer: 3.581 ± 0.606
3.727ThrThr: 3.727 ± 0.643
6.212ThrVal: 6.212 ± 0.854
0.877ThrTrp: 0.877 ± 0.265
1.462ThrTyr: 1.462 ± 0.375
0.0ThrXaa: 0.0 ± 0.0
Val
7.893ValAla: 7.893 ± 0.8
0.731ValCys: 0.731 ± 0.236
4.166ValAsp: 4.166 ± 0.637
6.285ValGlu: 6.285 ± 0.683
1.535ValPhe: 1.535 ± 0.362
5.335ValGly: 5.335 ± 0.546
1.023ValHis: 1.023 ± 0.243
3.435ValIle: 3.435 ± 0.51
2.85ValLys: 2.85 ± 0.563
4.458ValLeu: 4.458 ± 0.585
0.95ValMet: 0.95 ± 0.272
2.996ValAsn: 2.996 ± 0.516
4.385ValPro: 4.385 ± 0.477
1.973ValGln: 1.973 ± 0.322
4.97ValArg: 4.97 ± 0.565
4.093ValSer: 4.093 ± 0.45
5.847ValThr: 5.847 ± 0.745
6.504ValVal: 6.504 ± 0.694
1.096ValTrp: 1.096 ± 0.224
1.827ValTyr: 1.827 ± 0.433
0.0ValXaa: 0.0 ± 0.0
Trp
1.9TrpAla: 1.9 ± 0.373
0.804TrpCys: 0.804 ± 0.293
1.169TrpAsp: 1.169 ± 0.255
0.219TrpGlu: 0.219 ± 0.118
0.512TrpPhe: 0.512 ± 0.231
1.242TrpGly: 1.242 ± 0.295
0.512TrpHis: 0.512 ± 0.168
1.096TrpIle: 1.096 ± 0.293
0.365TrpLys: 0.365 ± 0.15
2.046TrpLeu: 2.046 ± 0.406
0.292TrpMet: 0.292 ± 0.133
0.585TrpAsn: 0.585 ± 0.179
1.096TrpPro: 1.096 ± 0.332
0.804TrpGln: 0.804 ± 0.243
1.681TrpArg: 1.681 ± 0.298
0.804TrpSer: 0.804 ± 0.262
1.169TrpThr: 1.169 ± 0.272
1.827TrpVal: 1.827 ± 0.389
0.585TrpTrp: 0.585 ± 0.172
0.512TrpTyr: 0.512 ± 0.192
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.193TyrAla: 2.193 ± 0.329
0.365TyrCys: 0.365 ± 0.172
2.119TyrAsp: 2.119 ± 0.401
1.754TyrGlu: 1.754 ± 0.364
0.512TyrPhe: 0.512 ± 0.193
2.485TyrGly: 2.485 ± 0.468
0.804TyrHis: 0.804 ± 0.277
0.877TyrIle: 0.877 ± 0.234
0.658TyrLys: 0.658 ± 0.236
1.389TyrLeu: 1.389 ± 0.244
0.439TyrMet: 0.439 ± 0.184
0.877TyrAsn: 0.877 ± 0.22
0.877TyrPro: 0.877 ± 0.209
0.658TyrGln: 0.658 ± 0.181
1.389TyrArg: 1.389 ± 0.287
0.95TyrSer: 0.95 ± 0.242
1.973TyrThr: 1.973 ± 0.352
2.412TyrVal: 2.412 ± 0.537
0.512TyrTrp: 0.512 ± 0.271
0.731TyrTyr: 0.731 ± 0.213
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (13684 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski