African horse sickness virus 1 (AHSV-1)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus; African horse sickness virus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7WPQ7|A7WPQ7_AHSV1 Non-structural protein NS2 OS=African horse sickness virus 1 OX=33714 GN=NS2 PE=4 SV=1
MM1 pKa = 7.73DD2 pKa = 6.41AIAARR7 pKa = 11.84ALSVVRR13 pKa = 11.84ACVTVTDD20 pKa = 4.54ARR22 pKa = 11.84VSLDD26 pKa = 3.45PGVMEE31 pKa = 4.58TLGIAINRR39 pKa = 11.84YY40 pKa = 9.5NGLTNHH46 pKa = 5.97SVSMRR51 pKa = 11.84PQTQAEE57 pKa = 4.13RR58 pKa = 11.84NEE60 pKa = 4.13MFFMCTDD67 pKa = 3.34MVLAALNVQIGNISPDD83 pKa = 3.27YY84 pKa = 10.33DD85 pKa = 3.29QALATVGALATTEE98 pKa = 3.61IPYY101 pKa = 10.23NVQAMNDD108 pKa = 3.14IVRR111 pKa = 11.84ITGQMQTFGPSKK123 pKa = 10.5VQTGPYY129 pKa = 9.02AGAVEE134 pKa = 4.57VQQSGRR140 pKa = 11.84YY141 pKa = 7.76YY142 pKa = 10.0VPQGRR147 pKa = 11.84TRR149 pKa = 11.84GGYY152 pKa = 9.08INSNIAEE159 pKa = 4.2VCMDD163 pKa = 4.42AGAAGQVNGLLAPRR177 pKa = 11.84RR178 pKa = 11.84GDD180 pKa = 2.91AVMIYY185 pKa = 8.43FVWRR189 pKa = 11.84PLRR192 pKa = 11.84IFCDD196 pKa = 3.62PQGASLEE203 pKa = 4.41SAPGTFVTVDD213 pKa = 3.69GVNVAAGDD221 pKa = 3.79VVAWNTIAPVNVGNPGARR239 pKa = 11.84RR240 pKa = 11.84SILQFEE246 pKa = 4.69VLWYY250 pKa = 9.47TSLDD254 pKa = 3.49RR255 pKa = 11.84SLDD258 pKa = 3.83TVPEE262 pKa = 4.24LAPTLTRR269 pKa = 11.84CYY271 pKa = 10.57AYY273 pKa = 10.75VSPTWHH279 pKa = 7.46ALRR282 pKa = 11.84AVIFQQMNMQPINPPIFPPTEE303 pKa = 3.78RR304 pKa = 11.84NEE306 pKa = 3.78IVAYY310 pKa = 10.61LLVASLADD318 pKa = 3.29VYY320 pKa = 11.39AALRR324 pKa = 11.84PDD326 pKa = 3.02FRR328 pKa = 11.84MNGVVAPVGQINRR341 pKa = 11.84ALVLAAYY348 pKa = 8.87HH349 pKa = 6.25

Molecular weight: 37.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C3TUP3|C3TUP3_AHSV1 Non-structural protein NS3 OS=African horse sickness virus 1 OX=33714 GN=NS3 PE=3 SV=1
MM1 pKa = 7.3NLASISQSYY10 pKa = 8.78MSHH13 pKa = 6.26NEE15 pKa = 3.73NEE17 pKa = 4.18RR18 pKa = 11.84SIVPYY23 pKa = 10.07IPPPYY28 pKa = 10.05HH29 pKa = 5.98PTAPALAVSASQMEE43 pKa = 4.53TMSLGILNQAMSSSAGASGALKK65 pKa = 10.45DD66 pKa = 3.76EE67 pKa = 4.47KK68 pKa = 11.13AAFGAVAEE76 pKa = 4.41ALRR79 pKa = 11.84DD80 pKa = 3.68PEE82 pKa = 4.88PIRR85 pKa = 11.84KK86 pKa = 8.91IKK88 pKa = 10.12RR89 pKa = 11.84QVGIQTLKK97 pKa = 8.19TLKK100 pKa = 10.67VEE102 pKa = 4.19LSGMRR107 pKa = 11.84RR108 pKa = 11.84KK109 pKa = 10.13KK110 pKa = 10.76LILKK114 pKa = 10.02IIMFICANVTMATSLVGGMSIVDD137 pKa = 3.38EE138 pKa = 5.63DD139 pKa = 3.91IAKK142 pKa = 10.11HH143 pKa = 5.6LAFDD147 pKa = 4.62GKK149 pKa = 10.99GDD151 pKa = 3.67WVSKK155 pKa = 8.86TVHH158 pKa = 6.1GLNLLCTTMLLAANKK173 pKa = 9.25ISEE176 pKa = 4.38KK177 pKa = 9.96VRR179 pKa = 11.84EE180 pKa = 4.17EE181 pKa = 3.44IARR184 pKa = 11.84TKK186 pKa = 10.66RR187 pKa = 11.84DD188 pKa = 2.99IAKK191 pKa = 9.01RR192 pKa = 11.84QSYY195 pKa = 10.11VSAATKK201 pKa = 10.23SWDD204 pKa = 3.49GDD206 pKa = 4.1SVTLLRR212 pKa = 11.84DD213 pKa = 3.47VKK215 pKa = 11.28YY216 pKa = 11.21GDD218 pKa = 3.44

Molecular weight: 23.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

6470

208

1305

588.2

66.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.862 ± 0.786

1.314 ± 0.19

6.121 ± 0.264

6.909 ± 0.504

3.833 ± 0.425

6.275 ± 0.708

1.963 ± 0.241

6.909 ± 0.388

6.151 ± 0.663

8.485 ± 0.352

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.539 ± 0.234

4.111 ± 0.326

3.663 ± 0.377

3.447 ± 0.323

6.955 ± 0.334

6.182 ± 0.493

5.487 ± 0.305

6.785 ± 0.338

1.283 ± 0.21

3.725 ± 0.327
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski