Circular ssDNA virus sp.

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A193CEC3|A0A193CEC3_9VIRU ATP-dependent helicase Rep OS=Circular ssDNA virus sp. OX=2805939 GN=rep PE=3 SV=1
MM1 pKa = 7.38SLDD4 pKa = 3.66GDD6 pKa = 3.88HH7 pKa = 7.06CGEE10 pKa = 5.01CGSDD14 pKa = 3.17PCSCVVQDD22 pKa = 4.66EE23 pKa = 4.66PKK25 pKa = 9.58TPAGKK30 pKa = 10.26RR31 pKa = 11.84PLSASQYY38 pKa = 9.31IDD40 pKa = 3.05VDD42 pKa = 4.11AEE44 pKa = 4.52DD45 pKa = 4.64GSQPEE50 pKa = 4.42SIEE53 pKa = 4.04GEE55 pKa = 4.16EE56 pKa = 3.88EE57 pKa = 4.46DD58 pKa = 5.17EE59 pKa = 4.51VLEE62 pKa = 4.1HH63 pKa = 6.7HH64 pKa = 6.68KK65 pKa = 10.68GYY67 pKa = 10.83HH68 pKa = 4.81KK69 pKa = 9.69TFEE72 pKa = 4.17KK73 pKa = 10.7KK74 pKa = 10.0LRR76 pKa = 11.84CDD78 pKa = 3.12EE79 pKa = 4.59RR80 pKa = 11.84YY81 pKa = 10.32SPEE84 pKa = 4.41ADD86 pKa = 2.88LDD88 pKa = 3.96AYY90 pKa = 10.79FDD92 pKa = 3.89EE93 pKa = 5.78FGLSAEE99 pKa = 4.67SRR101 pKa = 11.84IAMCRR106 pKa = 11.84TYY108 pKa = 11.51ANYY111 pKa = 8.48LTQKK115 pKa = 9.78LRR117 pKa = 11.84SSGRR121 pKa = 11.84LGPARR126 pKa = 11.84PKK128 pKa = 10.82KK129 pKa = 10.35LGQKK133 pKa = 9.1TDD135 pKa = 3.27TQRR138 pKa = 11.84RR139 pKa = 11.84LTWKK143 pKa = 10.37

Molecular weight: 16.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A193CDU9|A0A193CDU9_9VIRU Uncharacterized protein OS=Circular ssDNA virus sp. OX=2805939 GN=hyp PE=4 SV=1
MM1 pKa = 7.17SVSRR5 pKa = 11.84SRR7 pKa = 11.84TRR9 pKa = 11.84SPSAARR15 pKa = 11.84AGDD18 pKa = 3.77YY19 pKa = 10.89APWGPGVRR27 pKa = 11.84GGGGFYY33 pKa = 10.73APTPGEE39 pKa = 3.86TKK41 pKa = 10.87YY42 pKa = 11.02FDD44 pKa = 3.34TSFSQTISAAADD56 pKa = 3.28WSGSEE61 pKa = 4.51VPCTNYY67 pKa = 9.81IQSDD71 pKa = 4.06GTTVGAYY78 pKa = 8.16TDD80 pKa = 3.61SALIPSSVGAGYY92 pKa = 9.89GQVVGSKK99 pKa = 10.34FFLKK103 pKa = 10.43KK104 pKa = 10.3LRR106 pKa = 11.84VRR108 pKa = 11.84GNVASAVGSDD118 pKa = 3.6QADD121 pKa = 3.43VPLPQSCRR129 pKa = 11.84LTLVQDD135 pKa = 3.67TRR137 pKa = 11.84PNGAQAQGEE146 pKa = 4.52EE147 pKa = 4.69VFTDD151 pKa = 3.46MGAISQVHH159 pKa = 5.98HH160 pKa = 6.81SFLAMGAGQGSRR172 pKa = 11.84FRR174 pKa = 11.84ILSDD178 pKa = 3.41EE179 pKa = 4.3FVDD182 pKa = 4.8LQPAVAGTDD191 pKa = 3.58GTNTNSVSRR200 pKa = 11.84TGYY203 pKa = 7.88TFSMVYY209 pKa = 10.01KK210 pKa = 9.19PKK212 pKa = 10.79KK213 pKa = 8.74PIQVFLKK220 pKa = 10.36ANSSTPTIASLSTT233 pKa = 3.33

Molecular weight: 24.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

716

143

340

238.7

26.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.704 ± 1.489

2.235 ± 0.668

5.587 ± 0.992

6.425 ± 1.885

4.888 ± 0.891

8.38 ± 1.272

3.352 ± 1.298

3.911 ± 0.848

6.006 ± 1.119

6.285 ± 0.686

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.793 ± 0.809

2.793 ± 0.693

5.587 ± 0.206

5.028 ± 0.233

6.145 ± 0.435

7.263 ± 2.694

5.866 ± 1.218

5.866 ± 1.087

1.257 ± 0.31

3.631 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski