Caulobacter phage Ccr5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dolichocephalovirinae; Shapirovirus; unclassified Shapirovirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 321 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0EBM8|A0A1V0EBM8_9CAUD Uncharacterized protein OS=Caulobacter phage Ccr5 OX=1959740 GN=Ccr5_gp097 PE=4 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84EE3 pKa = 3.81GFMLEE8 pKa = 4.36GYY10 pKa = 9.94GGLKK14 pKa = 9.79IGPGGGADD22 pKa = 3.82PYY24 pKa = 8.94TTLGDD29 pKa = 4.04GGSEE33 pKa = 3.97GCIPVKK39 pKa = 10.77DD40 pKa = 5.34DD41 pKa = 3.22IAPDD45 pKa = 3.55GGTEE49 pKa = 3.9IISPHH54 pKa = 6.43GYY56 pKa = 9.87GG57 pKa = 4.0

Molecular weight: 5.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0EBY3|A0A1V0EBY3_9CAUD Antirestriction protein OS=Caulobacter phage Ccr5 OX=1959740 GN=Ccr5_gp195c PE=4 SV=1
MM1 pKa = 7.46SSSEE5 pKa = 4.23VLVQDD10 pKa = 4.79PLPPAAPNRR19 pKa = 11.84RR20 pKa = 11.84HH21 pKa = 6.59RR22 pKa = 11.84LFLEE26 pKa = 4.17AGGLCTYY33 pKa = 8.65CTGQMAYY40 pKa = 9.87RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84IGRR46 pKa = 11.84GGGKK50 pKa = 9.88LEE52 pKa = 4.32PDD54 pKa = 3.44EE55 pKa = 4.17VTIEE59 pKa = 4.04HH60 pKa = 6.63LEE62 pKa = 3.84ARR64 pKa = 11.84AEE66 pKa = 4.25GGSNHH71 pKa = 6.27EE72 pKa = 4.65SNLAAACVACNNTKK86 pKa = 10.81DD87 pKa = 3.64NAMPAWEE94 pKa = 4.18FRR96 pKa = 11.84RR97 pKa = 11.84LRR99 pKa = 11.84LRR101 pKa = 11.84LLPEE105 pKa = 4.32WPPCTFPPLSVRR117 pKa = 11.84RR118 pKa = 11.84RR119 pKa = 11.84EE120 pKa = 3.87PGLRR124 pKa = 11.84PVSKK128 pKa = 10.59RR129 pKa = 11.84IGRR132 pKa = 11.84LFEE135 pKa = 5.85NIDD138 pKa = 3.29NPGYY142 pKa = 7.46WRR144 pKa = 11.84RR145 pKa = 11.84WFGSDD150 pKa = 3.09PTRR153 pKa = 11.84KK154 pKa = 9.3VALVLHH160 pKa = 6.58RR161 pKa = 11.84EE162 pKa = 3.96QRR164 pKa = 11.84HH165 pKa = 4.45LAIKK169 pKa = 10.3RR170 pKa = 11.84RR171 pKa = 11.84VLLEE175 pKa = 3.91HH176 pKa = 6.82LPGRR180 pKa = 11.84NDD182 pKa = 2.81SRR184 pKa = 11.84TVVFWRR190 pKa = 11.84FGPLSAIKK198 pKa = 10.53VITGRR203 pKa = 11.84SAAYY207 pKa = 9.55EE208 pKa = 4.03DD209 pKa = 4.14RR210 pKa = 11.84DD211 pKa = 4.04LVLSLSWVWRR221 pKa = 11.84GADD224 pKa = 3.45VLTALLGRR232 pKa = 11.84ARR234 pKa = 11.84SAEE237 pKa = 4.13TTPGGEE243 pKa = 4.09PP244 pKa = 3.31

Molecular weight: 27.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

321

0

321

63071

24

2796

196.5

21.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.669 ± 0.238

0.913 ± 0.057

6.518 ± 0.153

6.309 ± 0.243

3.648 ± 0.094

8.227 ± 0.542

2.142 ± 0.112

4.562 ± 0.098

4.492 ± 0.198

8.281 ± 0.188

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.266 ± 0.096

2.863 ± 0.113

5.277 ± 0.142

3.212 ± 0.093

6.918 ± 0.251

4.963 ± 0.171

6.05 ± 0.27

6.91 ± 0.138

1.746 ± 0.069

3.035 ± 0.107
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski