Amino acid dipepetide frequency for Cynoglossus semilaevis (Tongue sole)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.121AlaAla: 6.121 ± 0.028	1.21AlaCys: 1.21 ± 0.009	3.051AlaAsp: 3.051 ± 0.013	4.366AlaGlu: 4.366 ± 0.02	2.304AlaPhe: 2.304 ± 0.012	4.039AlaGly: 4.039 ± 0.02	1.436AlaHis: 1.436 ± 0.01	2.556AlaIle: 2.556 ± 0.013	3.211AlaLys: 3.211 ± 0.018	6.241AlaLeu: 6.241 ± 0.023	1.484AlaMet: 1.484 ± 0.01	2.168AlaAsn: 2.168 ± 0.012	3.319AlaPro: 3.319 ± 0.019	2.792AlaGln: 2.792 ± 0.016	2.911AlaArg: 2.911 ± 0.014	5.109AlaSer: 5.109 ± 0.02	3.391AlaThr: 3.391 ± 0.013	4.854AlaVal: 4.854 ± 0.021	0.608AlaTrp: 0.608 ± 0.006	1.452AlaTyr: 1.452 ± 0.01	0.003AlaXaa: 0.003 ± 0.0
Cys	1.137CysAla: 1.137 ± 0.009	0.683CysCys: 0.683 ± 0.01	1.172CysAsp: 1.172 ± 0.012	1.266CysGlu: 1.266 ± 0.012	0.927CysPhe: 0.927 ± 0.007	1.571CysGly: 1.571 ± 0.015	0.685CysHis: 0.685 ± 0.008	0.965CysIle: 0.965 ± 0.009	1.125CysLys: 1.125 ± 0.01	2.273CysLeu: 2.273 ± 0.016	0.476CysMet: 0.476 ± 0.005	0.838CysAsn: 0.838 ± 0.008	1.376CysPro: 1.376 ± 0.014	1.072CysGln: 1.072 ± 0.011	1.311CysArg: 1.311 ± 0.012	2.248CysSer: 2.248 ± 0.015	1.226CysThr: 1.226 ± 0.01	1.648CysVal: 1.648 ± 0.014	0.318CysTrp: 0.318 ± 0.004	0.636CysTyr: 0.636 ± 0.007	0.001CysXaa: 0.001 ± 0.0
Asp	2.804AspAla: 2.804 ± 0.014	1.172AspCys: 1.172 ± 0.011	3.162AspAsp: 3.162 ± 0.024	3.671AspGlu: 3.671 ± 0.019	2.13AspPhe: 2.13 ± 0.013	3.592AspGly: 3.592 ± 0.02	1.256AspHis: 1.256 ± 0.01	2.672AspIle: 2.672 ± 0.015	2.753AspLys: 2.753 ± 0.013	5.048AspLeu: 5.048 ± 0.02	1.309AspMet: 1.309 ± 0.009	2.017AspAsn: 2.017 ± 0.011	2.776AspPro: 2.776 ± 0.013	2.003AspGln: 2.003 ± 0.011	2.772AspArg: 2.772 ± 0.016	4.441AspSer: 4.441 ± 0.018	2.766AspThr: 2.766 ± 0.017	3.413AspVal: 3.413 ± 0.015	0.657AspTrp: 0.657 ± 0.007	1.559AspTyr: 1.559 ± 0.01	0.002AspXaa: 0.002 ± 0.0
Glu	4.439GluAla: 4.439 ± 0.021	1.237GluCys: 1.237 ± 0.013	4.336GluAsp: 4.336 ± 0.02	7.777GluGlu: 7.777 ± 0.046	1.978GluPhe: 1.978 ± 0.011	3.935GluGly: 3.935 ± 0.018	1.446GluHis: 1.446 ± 0.01	2.796GluIle: 2.796 ± 0.015	4.792GluLys: 4.792 ± 0.031	6.078GluLeu: 6.078 ± 0.028	1.7GluMet: 1.7 ± 0.011	2.904GluAsn: 2.904 ± 0.016	2.738GluPro: 2.738 ± 0.016	3.15GluGln: 3.15 ± 0.02	4.09GluArg: 4.09 ± 0.023	4.265GluSer: 4.265 ± 0.019	3.46GluThr: 3.46 ± 0.014	4.312GluVal: 4.312 ± 0.019	0.679GluTrp: 0.679 ± 0.007	1.554GluTyr: 1.554 ± 0.011	0.002GluXaa: 0.002 ± 0.0
Phe	1.879PheAla: 1.879 ± 0.012	1.0PheCys: 1.0 ± 0.008	1.785PheAsp: 1.785 ± 0.011	1.86PheGlu: 1.86 ± 0.011	1.757PhePhe: 1.757 ± 0.013	2.21PheGly: 2.21 ± 0.014	1.056PheHis: 1.056 ± 0.008	1.941PheIle: 1.941 ± 0.013	1.849PheLys: 1.849 ± 0.012	3.878PheLeu: 3.878 ± 0.018	0.84PheMet: 0.84 ± 0.008	1.538PheAsn: 1.538 ± 0.009	1.866PhePro: 1.866 ± 0.011	1.634PheGln: 1.634 ± 0.01	1.865PheArg: 1.865 ± 0.012	3.493PheSer: 3.493 ± 0.017	2.356PheThr: 2.356 ± 0.014	2.3PheVal: 2.3 ± 0.015	0.469PheTrp: 0.469 ± 0.005	1.247PheTyr: 1.247 ± 0.008	0.002PheXaa: 0.002 ± 0.0
Gly	3.873GlyAla: 3.873 ± 0.019	1.258GlyCys: 1.258 ± 0.009	3.213GlyAsp: 3.213 ± 0.018	3.933GlyGlu: 3.933 ± 0.021	2.444GlyPhe: 2.444 ± 0.017	5.284GlyGly: 5.284 ± 0.037	1.714GlyHis: 1.714 ± 0.012	2.577GlyIle: 2.577 ± 0.013	3.491GlyLys: 3.491 ± 0.019	5.488GlyLeu: 5.488 ± 0.023	1.419GlyMet: 1.419 ± 0.011	2.445GlyAsn: 2.445 ± 0.014	3.2GlyPro: 3.2 ± 0.037	2.797GlyGln: 2.797 ± 0.015	3.559GlyArg: 3.559 ± 0.019	5.682GlySer: 5.682 ± 0.03	3.396GlyThr: 3.396 ± 0.019	4.001GlyVal: 4.001 ± 0.028	0.765GlyTrp: 0.765 ± 0.008	1.813GlyTyr: 1.813 ± 0.013	0.004GlyXaa: 0.004 ± 0.0
His	1.273HisAla: 1.273 ± 0.01	0.801HisCys: 0.801 ± 0.008	1.002HisAsp: 1.002 ± 0.008	1.226HisGlu: 1.226 ± 0.009	1.1HisPhe: 1.1 ± 0.007	1.587HisGly: 1.587 ± 0.013	1.149HisHis: 1.149 ± 0.014	1.319HisIle: 1.319 ± 0.009	1.303HisLys: 1.303 ± 0.01	2.805HisLeu: 2.805 ± 0.014	0.674HisMet: 0.674 ± 0.008	1.069HisAsn: 1.069 ± 0.007	1.576HisPro: 1.576 ± 0.012	1.381HisGln: 1.381 ± 0.012	1.729HisArg: 1.729 ± 0.012	2.555HisSer: 2.555 ± 0.013	1.694HisThr: 1.694 ± 0.016	1.534HisVal: 1.534 ± 0.01	0.349HisTrp: 0.349 ± 0.005	0.858HisTyr: 0.858 ± 0.008	0.001HisXaa: 0.001 ± 0.0
Ile	2.379IleAla: 2.379 ± 0.014	1.107IleCys: 1.107 ± 0.01	2.084IleAsp: 2.084 ± 0.012	2.328IleGlu: 2.328 ± 0.014	1.835IlePhe: 1.835 ± 0.014	2.288IleGly: 2.288 ± 0.012	1.309IleHis: 1.309 ± 0.011	2.408IleIle: 2.408 ± 0.014	2.547IleLys: 2.547 ± 0.013	4.264IleLeu: 4.264 ± 0.017	1.066IleMet: 1.066 ± 0.008	2.002IleAsn: 2.002 ± 0.012	2.422IlePro: 2.422 ± 0.014	2.207IleGln: 2.207 ± 0.012	2.409IleArg: 2.409 ± 0.013	3.789IleSer: 3.789 ± 0.014	2.782IleThr: 2.782 ± 0.016	2.579IleVal: 2.579 ± 0.014	0.472IleTrp: 0.472 ± 0.006	1.446IleTyr: 1.446 ± 0.023	0.002IleXaa: 0.002 ± 0.0
Lys	3.532LysAla: 3.532 ± 0.017	1.061LysCys: 1.061 ± 0.009	3.283LysAsp: 3.283 ± 0.016	4.704LysGlu: 4.704 ± 0.025	1.616LysPhe: 1.616 ± 0.011	3.149LysGly: 3.149 ± 0.022	1.444LysHis: 1.444 ± 0.01	2.527LysIle: 2.527 ± 0.013	4.597LysLys: 4.597 ± 0.03	4.919LysLeu: 4.919 ± 0.023	1.5LysMet: 1.5 ± 0.01	2.413LysAsn: 2.413 ± 0.014	2.845LysPro: 2.845 ± 0.018	2.557LysGln: 2.557 ± 0.014	3.375LysArg: 3.375 ± 0.017	3.866LysSer: 3.866 ± 0.016	3.311LysThr: 3.311 ± 0.017	3.631LysVal: 3.631 ± 0.015	0.566LysTrp: 0.566 ± 0.005	1.487LysTyr: 1.487 ± 0.011	0.003LysXaa: 0.003 ± 0.0
Leu	5.699LeuAla: 5.699 ± 0.023	2.291LeuCys: 2.291 ± 0.014	4.88LeuAsp: 4.88 ± 0.02	6.377LeuGlu: 6.377 ± 0.031	3.5LeuPhe: 3.5 ± 0.019	5.088LeuGly: 5.088 ± 0.024	2.778LeuHis: 2.778 ± 0.014	3.793LeuIle: 3.793 ± 0.016	5.586LeuLys: 5.586 ± 0.025	10.132LeuLeu: 10.132 ± 0.038	2.189LeuMet: 2.189 ± 0.013	3.618LeuAsn: 3.618 ± 0.016	5.256LeuPro: 5.256 ± 0.025	5.529LeuGln: 5.529 ± 0.029	5.57LeuArg: 5.57 ± 0.022	8.476LeuSer: 8.476 ± 0.029	5.437LeuThr: 5.437 ± 0.02	5.546LeuVal: 5.546 ± 0.022	1.083LeuTrp: 1.083 ± 0.009	2.609LeuTyr: 2.609 ± 0.013	0.006LeuXaa: 0.006 ± 0.001
Met	1.873MetAla: 1.873 ± 0.011	0.504MetCys: 0.504 ± 0.006	1.462MetAsp: 1.462 ± 0.01	1.989MetGlu: 1.989 ± 0.011	0.874MetPhe: 0.874 ± 0.008	1.391MetGly: 1.391 ± 0.011	0.478MetHis: 0.478 ± 0.005	0.898MetIle: 0.898 ± 0.008	1.482MetLys: 1.482 ± 0.009	2.063MetLeu: 2.063 ± 0.012	0.733MetMet: 0.733 ± 0.007	0.942MetAsn: 0.942 ± 0.007	1.026MetPro: 1.026 ± 0.009	0.976MetGln: 0.976 ± 0.009	1.147MetArg: 1.147 ± 0.008	2.001MetSer: 2.001 ± 0.011	1.344MetThr: 1.344 ± 0.008	1.585MetVal: 1.585 ± 0.01	0.275MetTrp: 0.275 ± 0.004	0.64MetTyr: 0.64 ± 0.006	0.001MetXaa: 0.001 ± 0.0
Asn	2.111AsnAla: 2.111 ± 0.012	0.905AsnCys: 0.905 ± 0.008	1.772AsnAsp: 1.772 ± 0.011	2.055AsnGlu: 2.055 ± 0.01	1.454AsnPhe: 1.454 ± 0.011	2.77AsnGly: 2.77 ± 0.019	1.069AsnHis: 1.069 ± 0.008	2.202AsnIle: 2.202 ± 0.013	2.305AsnLys: 2.305 ± 0.011	3.685AsnLeu: 3.685 ± 0.017	1.099AsnMet: 1.099 ± 0.008	1.977AsnAsn: 1.977 ± 0.014	2.252AsnPro: 2.252 ± 0.014	1.841AsnGln: 1.841 ± 0.012	2.052AsnArg: 2.052 ± 0.011	3.437AsnSer: 3.437 ± 0.016	2.404AsnThr: 2.404 ± 0.013	2.448AsnVal: 2.448 ± 0.014	0.446AsnTrp: 0.446 ± 0.005	1.165AsnTyr: 1.165 ± 0.008	0.001AsnXaa: 0.001 ± 0.0
Pro	3.768ProAla: 3.768 ± 0.02	1.106ProCys: 1.106 ± 0.012	2.829ProAsp: 2.829 ± 0.015	3.675ProGlu: 3.675 ± 0.018	1.797ProPhe: 1.797 ± 0.011	4.067ProGly: 4.067 ± 0.052	1.525ProHis: 1.525 ± 0.012	1.841ProIle: 1.841 ± 0.009	2.497ProLys: 2.497 ± 0.017	4.842ProLeu: 4.842 ± 0.017	1.013ProMet: 1.013 ± 0.009	1.933ProAsn: 1.933 ± 0.012	5.45ProPro: 5.45 ± 0.049	2.678ProGln: 2.678 ± 0.018	2.698ProArg: 2.698 ± 0.015	5.439ProSer: 5.439 ± 0.027	3.186ProThr: 3.186 ± 0.019	3.856ProVal: 3.856 ± 0.016	0.559ProTrp: 0.559 ± 0.006	1.426ProTyr: 1.426 ± 0.009	0.004ProXaa: 0.004 ± 0.0
Gln	2.977GlnAla: 2.977 ± 0.018	1.016GlnCys: 1.016 ± 0.01	2.278GlnAsp: 2.278 ± 0.012	3.541GlnGlu: 3.541 ± 0.019	1.38GlnPhe: 1.38 ± 0.01	2.696GlnGly: 2.696 ± 0.018	1.426GlnHis: 1.426 ± 0.013	1.956GlnIle: 1.956 ± 0.012	2.664GlnLys: 2.664 ± 0.014	4.548GlnLeu: 4.548 ± 0.025	1.185GlnMet: 1.185 ± 0.009	1.889GlnAsn: 1.889 ± 0.011	2.538GlnPro: 2.538 ± 0.018	3.563GlnGln: 3.563 ± 0.043	3.105GlnArg: 3.105 ± 0.017	3.592GlnSer: 3.592 ± 0.019	2.735GlnThr: 2.735 ± 0.013	2.901GlnVal: 2.901 ± 0.015	0.565GlnTrp: 0.565 ± 0.006	1.241GlnTyr: 1.241 ± 0.008	0.003GlnXaa: 0.003 ± 0.0
Arg	3.231ArgAla: 3.231 ± 0.016	1.276ArgCys: 1.276 ± 0.012	2.895ArgAsp: 2.895 ± 0.016	3.767ArgGlu: 3.767 ± 0.02	1.968ArgPhe: 1.968 ± 0.011	3.424ArgGly: 3.424 ± 0.02	1.614ArgHis: 1.614 ± 0.01	2.375ArgIle: 2.375 ± 0.01	3.503ArgLys: 3.503 ± 0.02	5.248ArgLeu: 5.248 ± 0.018	1.298ArgMet: 1.298 ± 0.008	2.178ArgAsn: 2.178 ± 0.012	2.945ArgPro: 2.945 ± 0.019	2.672ArgGln: 2.672 ± 0.013	4.315ArgArg: 4.315 ± 0.024	4.595ArgSer: 4.595 ± 0.022	3.018ArgThr: 3.018 ± 0.013	3.272ArgVal: 3.272 ± 0.013	0.671ArgTrp: 0.671 ± 0.007	1.515ArgTyr: 1.515 ± 0.01	0.004ArgXaa: 0.004 ± 0.0
Ser	5.418SerAla: 5.418 ± 0.022	2.082SerCys: 2.082 ± 0.018	4.402SerAsp: 4.402 ± 0.02	4.921SerGlu: 4.921 ± 0.021	3.164SerPhe: 3.164 ± 0.015	5.579SerGly: 5.579 ± 0.025	2.31SerHis: 2.31 ± 0.013	3.363SerIle: 3.363 ± 0.017	3.995SerLys: 3.995 ± 0.019	8.385SerLeu: 8.385 ± 0.026	1.835SerMet: 1.835 ± 0.01	3.106SerAsn: 3.106 ± 0.014	5.922SerPro: 5.922 ± 0.032	3.882SerGln: 3.882 ± 0.021	4.557SerArg: 4.557 ± 0.021	10.65SerSer: 10.65 ± 0.057	5.122SerThr: 5.122 ± 0.023	5.68SerVal: 5.68 ± 0.021	1.014SerTrp: 1.014 ± 0.009	2.19SerTyr: 2.19 ± 0.012	0.004SerXaa: 0.004 ± 0.0
Thr	4.046ThrAla: 4.046 ± 0.017	1.42ThrCys: 1.42 ± 0.015	3.017ThrAsp: 3.017 ± 0.014	3.812ThrGlu: 3.812 ± 0.017	2.206ThrPhe: 2.206 ± 0.013	3.796ThrGly: 3.796 ± 0.023	1.527ThrHis: 1.527 ± 0.015	2.456ThrIle: 2.456 ± 0.014	2.795ThrLys: 2.795 ± 0.014	5.461ThrLeu: 5.461 ± 0.019	1.266ThrMet: 1.266 ± 0.01	2.083ThrAsn: 2.083 ± 0.012	3.619ThrPro: 3.619 ± 0.022	2.459ThrGln: 2.459 ± 0.014	2.566ThrArg: 2.566 ± 0.014	5.086ThrSer: 5.086 ± 0.024	3.629ThrThr: 3.629 ± 0.029	4.376ThrVal: 4.376 ± 0.02	0.69ThrTrp: 0.69 ± 0.007	1.483ThrTyr: 1.483 ± 0.01	0.003ThrXaa: 0.003 ± 0.0
Val	4.042ValAla: 4.042 ± 0.018	1.83ValCys: 1.83 ± 0.014	3.325ValAsp: 3.325 ± 0.017	4.12ValGlu: 4.12 ± 0.016	2.708ValPhe: 2.708 ± 0.013	3.59ValGly: 3.59 ± 0.017	1.675ValHis: 1.675 ± 0.011	3.016ValIle: 3.016 ± 0.016	3.705ValLys: 3.705 ± 0.019	6.295ValLeu: 6.295 ± 0.028	1.558ValMet: 1.558 ± 0.009	2.593ValAsn: 2.593 ± 0.014	3.343ValPro: 3.343 ± 0.015	2.85ValGln: 2.85 ± 0.015	3.324ValArg: 3.324 ± 0.016	5.531ValSer: 5.531 ± 0.021	4.141ValThr: 4.141 ± 0.019	4.512ValVal: 4.512 ± 0.02	0.784ValTrp: 0.784 ± 0.008	1.822ValTyr: 1.822 ± 0.012	0.003ValXaa: 0.003 ± 0.0
Trp	0.648TrpAla: 0.648 ± 0.007	0.25TrpCys: 0.25 ± 0.004	0.633TrpAsp: 0.633 ± 0.006	0.726TrpGlu: 0.726 ± 0.007	0.481TrpPhe: 0.481 ± 0.006	0.624TrpGly: 0.624 ± 0.007	0.273TrpHis: 0.273 ± 0.004	0.545TrpIle: 0.545 ± 0.007	0.721TrpLys: 0.721 ± 0.007	1.153TrpLeu: 1.153 ± 0.009	0.353TrpMet: 0.353 ± 0.005	0.53TrpAsn: 0.53 ± 0.006	0.428TrpPro: 0.428 ± 0.005	0.492TrpGln: 0.492 ± 0.005	0.733TrpArg: 0.733 ± 0.007	0.992TrpSer: 0.992 ± 0.009	0.744TrpThr: 0.744 ± 0.008	0.684TrpVal: 0.684 ± 0.007	0.194TrpTrp: 0.194 ± 0.004	0.331TrpTyr: 0.331 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.375TyrAla: 1.375 ± 0.011	0.742TyrCys: 0.742 ± 0.008	1.395TyrAsp: 1.395 ± 0.01	1.55TyrGlu: 1.55 ± 0.01	1.202TyrPhe: 1.202 ± 0.011	1.671TyrGly: 1.671 ± 0.012	0.814TyrHis: 0.814 ± 0.008	1.517TyrIle: 1.517 ± 0.021	1.462TyrLys: 1.462 ± 0.012	2.63TyrLeu: 2.63 ± 0.015	0.687TyrMet: 0.687 ± 0.007	1.204TyrAsn: 1.204 ± 0.008	1.286TyrPro: 1.286 ± 0.01	1.263TyrGln: 1.263 ± 0.008	1.693TyrArg: 1.693 ± 0.011	2.329TyrSer: 2.329 ± 0.011	1.651TyrThr: 1.651 ± 0.01	1.632TyrVal: 1.632 ± 0.011	0.369TyrTrp: 0.369 ± 0.005	0.97TyrTyr: 0.97 ± 0.009	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.0	0.002XaaCys: 0.002 ± 0.0	0.002XaaAsp: 0.002 ± 0.0	0.004XaaGlu: 0.004 ± 0.0	0.002XaaPhe: 0.002 ± 0.0	0.003XaaGly: 0.003 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.002XaaIle: 0.002 ± 0.0	0.002XaaLys: 0.002 ± 0.0	0.006XaaLeu: 0.006 ± 0.001	0.002XaaMet: 0.002 ± 0.0	0.002XaaAsn: 0.002 ± 0.0	0.003XaaPro: 0.003 ± 0.0	0.002XaaGln: 0.002 ± 0.0	0.003XaaArg: 0.003 ± 0.0	0.005XaaSer: 0.005 ± 0.001	0.002XaaThr: 0.002 ± 0.0	0.004XaaVal: 0.004 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.992XaaXaa: 0.992 ± 0.075

Statistics based on 32408 proteins (18528367 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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