Roseburia sp. 1XD42-69

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; unclassified Roseburia

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4659 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9GPV4|A0A3A9GPV4_9FIRM TRAP transporter small permease OS=Roseburia sp. 1XD42-69 OX=2320088 GN=D7Y06_06935 PE=4 SV=1
MM1 pKa = 7.36NFLDD5 pKa = 4.5TFLKK9 pKa = 10.51DD10 pKa = 3.64YY11 pKa = 10.8EE12 pKa = 4.38RR13 pKa = 11.84EE14 pKa = 4.07DD15 pKa = 3.87YY16 pKa = 11.36EE17 pKa = 5.92DD18 pKa = 3.25ILEE21 pKa = 4.19IAEE24 pKa = 4.81AISANHH30 pKa = 6.68EE31 pKa = 4.04DD32 pKa = 4.75VITHH36 pKa = 6.44LQSALDD42 pKa = 3.66NPKK45 pKa = 10.75EE46 pKa = 3.96YY47 pKa = 10.2FQKK50 pKa = 10.09NAHH53 pKa = 6.48RR54 pKa = 11.84FHH56 pKa = 6.96EE57 pKa = 4.35RR58 pKa = 11.84NIDD61 pKa = 3.71LEE63 pKa = 4.29NGEE66 pKa = 4.93LEE68 pKa = 5.37DD69 pKa = 6.2EE70 pKa = 4.72IDD72 pKa = 4.47LDD74 pKa = 4.69DD75 pKa = 5.25LFFLAMVDD83 pKa = 3.68EE84 pKa = 4.32LHH86 pKa = 7.62DD87 pKa = 3.64YY88 pKa = 11.02GYY90 pKa = 10.83LYY92 pKa = 10.72EE93 pKa = 4.86VDD95 pKa = 3.8WKK97 pKa = 11.26CSLEE101 pKa = 4.11DD102 pKa = 4.01FLWSLEE108 pKa = 3.84QLKK111 pKa = 10.01NYY113 pKa = 10.79AMISHH118 pKa = 7.79ILPDD122 pKa = 4.23LDD124 pKa = 3.9LDD126 pKa = 4.1KK127 pKa = 11.55NFNVDD132 pKa = 2.9MWIEE136 pKa = 4.25TINDD140 pKa = 3.71ALGGNAYY147 pKa = 9.24ICYY150 pKa = 10.22VDD152 pKa = 4.13IDD154 pKa = 4.24SDD156 pKa = 4.26SFPLIIASPEE166 pKa = 3.92TYY168 pKa = 10.54RR169 pKa = 11.84LIGEE173 pKa = 4.2NDD175 pKa = 3.13SS176 pKa = 3.63

Molecular weight: 20.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9GAE9|A0A3A9GAE9_9FIRM Mutator family transposase OS=Roseburia sp. 1XD42-69 OX=2320088 GN=D7Y06_25140 PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.56QPKK8 pKa = 9.32KK9 pKa = 7.54RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4659

0

4659

1320199

33

3075

283.4

32.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.765 ± 0.03

1.526 ± 0.017

5.444 ± 0.03

8.17 ± 0.042

4.374 ± 0.027

6.921 ± 0.032

1.686 ± 0.015

7.337 ± 0.033

7.597 ± 0.034

8.972 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.137 ± 0.02

4.61 ± 0.027

3.007 ± 0.021

3.288 ± 0.02

4.406 ± 0.023

5.943 ± 0.029

5.055 ± 0.029

6.538 ± 0.026

0.929 ± 0.014

4.295 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski