Arthrobacter phage Adolin

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Manhattanvirus; unclassified Manhattanvirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9L5R2|A0A6B9L5R2_9CAUD Uncharacterized protein OS=Arthrobacter phage Adolin OX=2686213 GN=48 PE=4 SV=1
MM1 pKa = 7.84DD2 pKa = 6.37LDD4 pKa = 5.38DD5 pKa = 6.63DD6 pKa = 4.55VDD8 pKa = 4.5LEE10 pKa = 4.29LDD12 pKa = 3.26EE13 pKa = 5.78RR14 pKa = 11.84EE15 pKa = 4.53YY16 pKa = 11.63VCEE19 pKa = 4.05TCNLIHH25 pKa = 6.85WRR27 pKa = 11.84GAPDD31 pKa = 3.65PCDD34 pKa = 3.41RR35 pKa = 11.84SS36 pKa = 3.51

Molecular weight: 4.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LHM4|A0A6B9LHM4_9CAUD DNA primase/helicase OS=Arthrobacter phage Adolin OX=2686213 GN=37 PE=4 SV=1
MM1 pKa = 7.24IHH3 pKa = 7.03GNRR6 pKa = 11.84DD7 pKa = 3.49DD8 pKa = 4.92LLWLAGLLEE17 pKa = 4.55GEE19 pKa = 4.75GTFDD23 pKa = 3.26AHH25 pKa = 6.21RR26 pKa = 11.84GKK28 pKa = 10.74YY29 pKa = 9.13PRR31 pKa = 11.84IRR33 pKa = 11.84LAMTDD38 pKa = 3.13RR39 pKa = 11.84DD40 pKa = 4.23VVGRR44 pKa = 11.84AASLMDD50 pKa = 3.63SKK52 pKa = 10.97IRR54 pKa = 11.84LSLHH58 pKa = 4.88VAPAKK63 pKa = 7.62PTWHH67 pKa = 6.42TEE69 pKa = 3.46ISGEE73 pKa = 3.92RR74 pKa = 11.84AAEE77 pKa = 3.97IMRR80 pKa = 11.84QILPFMGTRR89 pKa = 11.84RR90 pKa = 11.84SGRR93 pKa = 11.84IAEE96 pKa = 4.34VLGTHH101 pKa = 6.81HH102 pKa = 7.03FRR104 pKa = 11.84QKK106 pKa = 11.2AVIEE110 pKa = 4.31AGKK113 pKa = 10.63GSTPGPRR120 pKa = 11.84VSRR123 pKa = 11.84PAGVAKK129 pKa = 10.34PLTAAA134 pKa = 4.22

Molecular weight: 14.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

13162

36

837

185.4

20.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.407 ± 0.389

0.653 ± 0.137

6.481 ± 0.312

6.473 ± 0.324

3.077 ± 0.147

8.502 ± 0.375

1.846 ± 0.188

4.118 ± 0.285

4.239 ± 0.271

8.76 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.93 ± 0.177

2.766 ± 0.21

5.455 ± 0.292

2.697 ± 0.214

7.332 ± 0.478

5.402 ± 0.262

6.466 ± 0.374

7.058 ± 0.256

1.702 ± 0.131

2.636 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski