Pseudomonas phage Pf1 (Bacteriophage Pf1)

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Primolicivirus

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P25130|VG430_BPPF1 Uncharacterized protein ORF430 OS=Pseudomonas phage Pf1 OX=2011081 PE=4 SV=1
MM1 pKa = 7.58SIKK4 pKa = 9.83TLISVLRR11 pKa = 11.84VTLLTACLLPSLFFVRR27 pKa = 11.84SAIAGPYY34 pKa = 7.42IWEE37 pKa = 3.97VVMYY41 pKa = 9.58SSSGSSTPAEE51 pKa = 4.3ACEE54 pKa = 4.04KK55 pKa = 10.65ARR57 pKa = 11.84VVADD61 pKa = 5.44RR62 pKa = 11.84SPDD65 pKa = 3.16WNYY68 pKa = 10.64TSATPKK74 pKa = 10.33MNGLDD79 pKa = 3.38NSYY82 pKa = 10.9CSVVYY87 pKa = 9.5VSRR90 pKa = 11.84RR91 pKa = 11.84DD92 pKa = 3.42PSVVNTCDD100 pKa = 4.67DD101 pKa = 3.73CASWKK106 pKa = 10.32LFRR109 pKa = 11.84KK110 pKa = 10.16GDD112 pKa = 3.54QCANADD118 pKa = 3.63DD119 pKa = 4.74TYY121 pKa = 11.35NASTGICEE129 pKa = 4.52PPPKK133 pKa = 9.72EE134 pKa = 4.2CKK136 pKa = 10.06EE137 pKa = 4.24GEE139 pKa = 4.18LFPAKK144 pKa = 10.51GPDD147 pKa = 3.63SPVVTSGGRR156 pKa = 11.84NYY158 pKa = 10.44VGDD161 pKa = 3.91GGAPTACYY169 pKa = 9.86QSCEE173 pKa = 4.11YY174 pKa = 10.76GGNPSPASCYY184 pKa = 9.54LVKK187 pKa = 10.94GSTTTGFCNYY197 pKa = 8.87ILKK200 pKa = 8.92GTGQSCGADD209 pKa = 2.95SYY211 pKa = 11.34TFSQTGDD218 pKa = 3.49SLNPPDD224 pKa = 5.13TPNTDD229 pKa = 3.25PSDD232 pKa = 4.35PNDD235 pKa = 4.31PGCPPGWSWSGTTCVKK251 pKa = 10.28TPTDD255 pKa = 3.83PTDD258 pKa = 3.68PTDD261 pKa = 3.54PTTPGGDD268 pKa = 3.12GGGDD272 pKa = 3.55GNGGGNNNGGGNDD285 pKa = 3.66GGTGNGDD292 pKa = 3.15GSGGGDD298 pKa = 3.31GNGAGDD304 pKa = 4.1GSGDD308 pKa = 3.61GDD310 pKa = 3.91GSGTGGDD317 pKa = 4.04GNGTCDD323 pKa = 3.69PAKK326 pKa = 9.63EE327 pKa = 4.12NCSTGPEE334 pKa = 4.5GPGGEE339 pKa = 4.44LKK341 pKa = 10.79EE342 pKa = 4.34PTPGTWDD349 pKa = 3.43DD350 pKa = 6.49AIATWEE356 pKa = 4.34KK357 pKa = 10.62KK358 pKa = 10.2VEE360 pKa = 4.02EE361 pKa = 4.22AKK363 pKa = 10.76KK364 pKa = 9.88EE365 pKa = 4.02LKK367 pKa = 9.93TKK369 pKa = 10.58VKK371 pKa = 11.07ANVDD375 pKa = 3.5QMKK378 pKa = 10.59GAFDD382 pKa = 4.66LNLAEE387 pKa = 5.82GGGQLPCEE395 pKa = 4.61SMTIWGKK402 pKa = 10.71SYY404 pKa = 10.76SLCISDD410 pKa = 3.97YY411 pKa = 11.17AGQLSSLRR419 pKa = 11.84VALLLMAALIAALILLKK436 pKa = 10.73DD437 pKa = 3.5

Molecular weight: 44.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P25137|VG030_BPPF1 3.2 kDa protein OS=Pseudomonas phage Pf1 OX=2011081 PE=4 SV=1
MM1 pKa = 7.36TKK3 pKa = 10.41YY4 pKa = 10.39LVEE7 pKa = 3.97ICTFHH12 pKa = 7.03GPTRR16 pKa = 11.84QRR18 pKa = 11.84RR19 pKa = 11.84WHH21 pKa = 6.39RR22 pKa = 11.84VHH24 pKa = 6.48QGGSRR29 pKa = 11.84VEE31 pKa = 3.96CQRR34 pKa = 11.84WVEE37 pKa = 3.93EE38 pKa = 4.06LVAVFPTEE46 pKa = 3.8EE47 pKa = 3.73EE48 pKa = 3.86ARR50 pKa = 11.84RR51 pKa = 11.84SFGLTRR57 pKa = 11.84EE58 pKa = 4.32RR59 pKa = 11.84ARR61 pKa = 11.84QVYY64 pKa = 10.08RR65 pKa = 11.84IRR67 pKa = 11.84GVRR70 pKa = 11.84AA71 pKa = 3.04

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

2574

30

437

183.9

20.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.868 ± 1.017

2.253 ± 0.496

5.478 ± 0.697

4.74 ± 0.52

3.341 ± 0.371

9.091 ± 1.207

2.214 ± 0.654

4.507 ± 0.573

4.274 ± 0.367

8.936 ± 0.818

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.098 ± 0.214

2.681 ± 0.482

6.41 ± 0.94

3.846 ± 0.549

7.265 ± 1.274

6.216 ± 0.655

5.633 ± 0.657

6.061 ± 0.488

1.981 ± 0.21

3.108 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski