Bacteroides sp. CAG:633
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3004 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R6FPN9|R6FPN9_9BACE Rhamnulokinase OS=Bacteroides sp. CAG:633 OX=1262744 GN=BN744_02636 PE=4 SV=1
MM1 pKa = 6.94 NTRR4 pKa = 11.84 NVNIPDD10 pKa = 3.85 IDD12 pKa = 4.8 DD13 pKa = 4.79 LDD15 pKa = 4.22 ALCRR19 pKa = 11.84 AIDD22 pKa = 3.41 WTQYY26 pKa = 11.24 QSMLEE31 pKa = 3.75 QSLANEE37 pKa = 4.26 KK38 pKa = 8.45 TWEE41 pKa = 4.29 FGCMDD46 pKa = 4.92 EE47 pKa = 5.12 YY48 pKa = 11.62 NPHH51 pKa = 6.56 TDD53 pKa = 3.15 NIAQIEE59 pKa = 4.36 EE60 pKa = 4.19 EE61 pKa = 4.78 LNFLSAGEE69 pKa = 4.12 YY70 pKa = 8.51 EE71 pKa = 5.1 AIVRR75 pKa = 11.84 MHH77 pKa = 6.86 DD78 pKa = 2.96 AEE80 pKa = 4.3 YY81 pKa = 10.45 FQDD84 pKa = 4.73 FVV86 pKa = 3.82
Molecular weight: 10.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.872
IPC_protein 3.795
Toseland 3.605
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.528
Solomon 3.757
Lehninger 3.719
Nozaki 3.91
DTASelect 4.075
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.923
Patrickios 0.846
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|R6FBJ2|R6FBJ2_9BACE Transporter gate domain protein OS=Bacteroides sp. CAG:633 OX=1262744 GN=BN744_00813 PE=4 SV=1
MM1 pKa = 7.37 EE2 pKa = 6.44 PIRR5 pKa = 11.84 NFDD8 pKa = 3.46 QLTAHH13 pKa = 7.46 LKK15 pKa = 8.81 TLNKK19 pKa = 9.33 RR20 pKa = 11.84 KK21 pKa = 10.05 RR22 pKa = 11.84 IAVVCANDD30 pKa = 3.83 PNTEE34 pKa = 4.08 YY35 pKa = 10.81 AISRR39 pKa = 11.84 ALEE42 pKa = 3.65 EE43 pKa = 5.14 GIAEE47 pKa = 4.11 FLMIGDD53 pKa = 3.91 SAILEE58 pKa = 4.56 KK59 pKa = 11.12 YY60 pKa = 9.74 PSLKK64 pKa = 10.53 NYY66 pKa = 9.05 PDD68 pKa = 3.84 YY69 pKa = 11.69 VHH71 pKa = 6.7 TLHH74 pKa = 7.86 VEE76 pKa = 4.41 DD77 pKa = 5.01 PDD79 pKa = 3.57 EE80 pKa = 4.43 AARR83 pKa = 11.84 EE84 pKa = 3.88 AVRR87 pKa = 11.84 IVRR90 pKa = 11.84 EE91 pKa = 4.18 GGADD95 pKa = 2.97 ILMKK99 pKa = 10.91 GIINTDD105 pKa = 3.45 NLLHH109 pKa = 7.4 AILDD113 pKa = 4.01 KK114 pKa = 11.34 EE115 pKa = 4.46 KK116 pKa = 11.14 GLLPKK121 pKa = 10.77 GKK123 pKa = 9.52 ILTHH127 pKa = 6.22 LAVMQIPTYY136 pKa = 10.94 DD137 pKa = 3.29 KK138 pKa = 11.43 LLFFSDD144 pKa = 3.25 AAVIPRR150 pKa = 11.84 PTLQQRR156 pKa = 11.84 IEE158 pKa = 4.28 MIWYY162 pKa = 8.73 AIRR165 pKa = 11.84 ACRR168 pKa = 11.84 HH169 pKa = 5.61 FGIEE173 pKa = 3.72 QPRR176 pKa = 11.84 XLRR179 pKa = 11.84 HH180 pKa = 5.6 RR181 pKa = 11.84 AAAHH185 pKa = 6.65 RR186 pKa = 11.84 PDD188 pKa = 3.37 TLYY191 pKa = 11.36 GEE193 pKa = 5.32 GEE195 pKa = 4.08 RR196 pKa = 11.84 QVPPFAGLCEE206 pKa = 3.93 HH207 pKa = 6.88 RR208 pKa = 11.84 RR209 pKa = 11.84 TGRR212 pKa = 11.84 SRR214 pKa = 11.84 RR215 pKa = 11.84 IRR217 pKa = 11.84 QRR219 pKa = 11.84 HH220 pKa = 4.44 HH221 pKa = 6.34 RR222 pKa = 11.84 RR223 pKa = 11.84 SAGRR227 pKa = 11.84 EE228 pKa = 3.62 NLVRR232 pKa = 11.84 EE233 pKa = 4.41 DD234 pKa = 3.31 QRR236 pKa = 11.84 RR237 pKa = 11.84 HH238 pKa = 3.75 QRR240 pKa = 11.84 HH241 pKa = 5.48 RR242 pKa = 11.84 FAHH245 pKa = 4.46 QRR247 pKa = 11.84 RR248 pKa = 11.84 GRR250 pKa = 11.84 RR251 pKa = 11.84 THH253 pKa = 7.03 LPQHH257 pKa = 6.17 RR258 pKa = 11.84 VGQRR262 pKa = 11.84 FLQSRR267 pKa = 11.84 LPVLARR273 pKa = 11.84 RR274 pKa = 11.84 HH275 pKa = 4.76 GRR277 pKa = 11.84 IAARR281 pKa = 11.84 PHH283 pKa = 6.09 LSGGAALAQRR293 pKa = 11.84 QRR295 pKa = 11.84 TVEE298 pKa = 4.2 VLQHH302 pKa = 5.59 RR303 pKa = 11.84 HH304 pKa = 5.51 GLPDD308 pKa = 3.43 EE309 pKa = 4.03 QRR311 pKa = 11.84 TGHH314 pKa = 5.76 EE315 pKa = 4.33 RR316 pKa = 11.84 IKK318 pKa = 10.86 QEE320 pKa = 3.63 ASRR323 pKa = 11.84 LTIHH327 pKa = 6.52 HH328 pKa = 7.16 LSFIIHH334 pKa = 6.76 HH335 pKa = 5.44 FHH337 pKa = 7.29 EE338 pKa = 6.1 DD339 pKa = 2.89 ISHH342 pKa = 5.46 QPRR345 pKa = 11.84 LYY347 pKa = 10.54 FDD349 pKa = 3.49 EE350 pKa = 4.66 DD351 pKa = 3.34 CRR353 pKa = 11.84 LRR355 pKa = 11.84 RR356 pKa = 3.75
Molecular weight: 41.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.589
IPC_protein 10.657
Toseland 10.73
ProMoST 10.76
Dawson 10.818
Bjellqvist 10.613
Wikipedia 11.096
Rodwell 10.833
Grimsley 10.877
Solomon 11.023
Lehninger 10.965
Nozaki 10.73
DTASelect 10.599
Thurlkill 10.745
EMBOSS 11.169
Sillero 10.76
Patrickios 10.511
IPC_peptide 11.023
IPC2_peptide 9.794
IPC2.peptide.svr19 8.774
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3004
0
3004
1032115
29
1910
343.6
38.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.514 ± 0.047
1.342 ± 0.017
5.622 ± 0.03
6.516 ± 0.043
4.447 ± 0.027
6.898 ± 0.04
1.989 ± 0.022
6.347 ± 0.053
6.021 ± 0.042
9.377 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.76 ± 0.02
4.771 ± 0.042
3.811 ± 0.027
3.718 ± 0.029
5.008 ± 0.038
5.875 ± 0.037
5.597 ± 0.033
6.674 ± 0.044
1.294 ± 0.019
4.415 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here