Colletotrichum salicis
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13783 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A135UHI2|A0A135UHI2_9PEZI Uncharacterized protein OS=Colletotrichum salicis OX=1209931 GN=CSAL01_05449 PE=4 SV=1
MM1 pKa = 7.98 DD2 pKa = 5.15 GSNTNSSYY10 pKa = 11.34 LLSEE14 pKa = 4.09 LNEE17 pKa = 4.37 EE18 pKa = 4.14 KK19 pKa = 10.6 RR20 pKa = 11.84 QTLRR24 pKa = 11.84 TLANLSEE31 pKa = 4.23 ADD33 pKa = 3.55 FRR35 pKa = 11.84 RR36 pKa = 11.84 ATFLATLHH44 pKa = 6.01 MISRR48 pKa = 11.84 GPHH51 pKa = 4.06 VTSTPLRR58 pKa = 11.84 EE59 pKa = 4.11 WQPPSTTTAGPSDD72 pKa = 3.77 NLDD75 pKa = 3.64 TEE77 pKa = 4.57 DD78 pKa = 4.28 LPGRR82 pKa = 11.84 SDD84 pKa = 3.73 FCNDD88 pKa = 3.07 ASCPCKK94 pKa = 10.58 SINSPADD101 pKa = 3.35 PSNNTAPQNAARR113 pKa = 11.84 GLFFGNDD120 pKa = 3.25 TMSTLDD126 pKa = 5.0 DD127 pKa = 4.42 EE128 pKa = 5.31 LCEE131 pKa = 4.17 DD132 pKa = 4.13 HH133 pKa = 7.66 ARR135 pKa = 11.84 MCGGEE140 pKa = 3.94 YY141 pKa = 10.04 CYY143 pKa = 10.62 EE144 pKa = 3.91 SSEE147 pKa = 4.3 YY148 pKa = 10.55 SDD150 pKa = 4.09 SSEE153 pKa = 4.23 SSGDD157 pKa = 3.56 YY158 pKa = 11.19 SEE160 pKa = 6.05 MEE162 pKa = 4.63 DD163 pKa = 4.58 LDD165 pKa = 4.77 DD166 pKa = 4.51 FGKK169 pKa = 10.6 ISVDD173 pKa = 2.93 VDD175 pKa = 3.65 DD176 pKa = 5.38 YY177 pKa = 12.04 VYY179 pKa = 11.43 LDD181 pKa = 3.67 ADD183 pKa = 3.89 GNEE186 pKa = 4.57 LDD188 pKa = 4.31 SEE190 pKa = 4.95 GPGLSDD196 pKa = 4.09 LSYY199 pKa = 11.04 DD200 pKa = 3.96 FEE202 pKa = 6.08 SMVCSNEE209 pKa = 3.76 DD210 pKa = 4.07 DD211 pKa = 4.1 EE212 pKa = 5.53 NKK214 pKa = 10.29 EE215 pKa = 3.92
Molecular weight: 23.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.783
IPC_protein 3.795
Toseland 3.567
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.478
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.706
Sillero 3.91
Patrickios 1.214
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A135S5S6|A0A135S5S6_9PEZI CFEM domain-containing protein OS=Colletotrichum salicis OX=1209931 GN=CSAL01_02869 PE=3 SV=1
MM1 pKa = 7.89 RR2 pKa = 11.84 LPLRR6 pKa = 11.84 SATSTPLTRR15 pKa = 11.84 TRR17 pKa = 11.84 LRR19 pKa = 11.84 SVMSTLSTSMRR30 pKa = 11.84 PLPP33 pKa = 4.12
Molecular weight: 3.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.43
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.974
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.149
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13783
0
13783
6540505
15
8731
474.5
52.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.058 ± 0.02
1.224 ± 0.009
5.716 ± 0.017
6.026 ± 0.024
3.763 ± 0.013
7.15 ± 0.021
2.316 ± 0.009
4.778 ± 0.015
4.817 ± 0.021
8.664 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.211 ± 0.01
3.672 ± 0.011
6.127 ± 0.027
3.877 ± 0.014
5.904 ± 0.025
8.078 ± 0.023
6.121 ± 0.029
6.255 ± 0.017
1.515 ± 0.009
2.713 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here